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Showing: 361 - 400 of 595 results

  1. Lenti sgRNA NGFR GFP out of frame

    Plasmid
    #155282
    Purpose
    Lentiviral plasmid for sgRNA, NGFR marker, editing detected with GFP, used with 155280
    Depositor
    Martin Carroll
    Insert
    GFP out of frame IRES NGFR
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    155282-plasmid-map-sequence-id-310437

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  2. Lenti sgRNA NGFR CFP out of frame

    Plasmid
    #155283
    Purpose
    Lentiviral plasmid for sgRNA, NGFR marker, editing detected with CFP, used with 155280
    Depositor
    Martin Carroll
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    155283-plasmid-map-sequence-id-309991

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  3. pOT_5 - lenti-EFS-FMC6.3-28z-2A-puro-2A-tNGFR

    Plasmid
    #181974
    Purpose
    Expresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.
    Depositor
    Neville Sanjana
    Article
    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:
    Insert
    FMC6.3-28z CAR; tNGFR (NGFR Human, Synthetic)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    NGFR truncation: aa 11-276
    Promoter
    Available since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    181974-plasmid-map-sequence-id-355578

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  4. pOT_6 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-tNGFR

    Plasmid
    #181975
    Purpose
    Expresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.
    Depositor
    Neville Sanjana
    Article
    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:
    Insert
    FMC6.3-BBz CAR; tNGFR (NGFR Human, Synthetic)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    NGFR truncation: aa 11-276
    Promoter
    Available since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    181975-plasmid-map-sequence-id-377682

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  7. pMB953:PB-CAG-PuroR-nGFP-FLEx-mCherry-KASH-WPRE

    Plasmid
    #168106
    Purpose
    PiggyBac transposon vector constitutively driving PuroR-P2A-eGFP and Cre conditional expression of KASH nuclear membrane-bound mCherry. Conditional insert flanked by MCS for cloning
    Depositor
    Erich Jarvis
    Article
    Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10
    Insert
    mCherry-KASH
    Use
    CRISPR and Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    CAG
    Available since
    May 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    168106-plasmid-map-sequence-id-330309

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  9. CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

    Plasmid
    #105842
    Purpose
    Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment
    Depositor
    Michael Ward
    Article
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    Optimized mApple
    rtTA3G
    SBP Delta-LNGFR
    Use
    CRISPR and TALEN
    Tags
    Expression
    Mutation
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    105842-plasmid-map-sequence-id-393690

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  10. pLV-MNTS-1

    Plasmid
    #182944
    Purpose
    To express a multi-neurotrophin made from the Human NT-3 gene. The secreted protein can bind all 3 TRK receptors and p75NTR
    Depositor
    Pantelis Tsoulfas
    Article
    Urfer et al EMBO J. 1994 Dec 15. 13(24):5896-909.
    Insert
    MNTS-1 (NTF3 Homo sapiens, Human, Synthetic)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    This is the Human NT-3 with mutations on the matu…
    Promoter
    CMV promoter and enhancer
    Available since
    April 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    182944-plasmid-map-sequence-id-360100

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  11. pCWXPGR-pTF-NT3

    Plasmid
    #114279
    Purpose
    TET-inducible expression of the Neurotrophin 3 protein
    Depositor
    Patrick Salmon
    Insert
    Neurotrophin 3 (Ntf3 Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114279-plasmid-map-sequence-id-218733

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  12. pcDNA3 NT3

    Plasmid
    #39981
    Depositor
    Yves-Alain Barde
    Insert
    Neurotrophin 3 (Ntf3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 21, 2012
    Availability
    Academic Institutions and Nonprofits only

  14. pXT

    Plasmid
    #107574
    Purpose
    Contains the VGF promoter (about 700 bp) plus 800 bp of 5' untranslated sequence. The HindIII (5')-HincII(3') fragment acts as a NGF-inducible promoter
    Depositor
    Andrea Levi
    Article
    Possenti et al Proc Natl Acad Sci U S A. 1992 May 1;89(
    Insert
    VGF promoter (Vgf Rat)
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107574-plasmid-map-sequence-id-205708

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  17. pLV-MNTS-1-p75

    Plasmid
    #182945
    Purpose
    To express a multi-neurotrophin made from the Human NT-3 gene. The secreted protein that can bind only all 3 TRK receptors and not p75NTR
    Depositor
    Pantelis Tsoulfas
    Article
    Urfer et al EMBO J. 1994 Dec 15. 13(24):5896-909.
    Insert
    MNTS-1-p75 (NTF3, NT3 Human, Synthetic)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter and enhancer
    Available since
    April 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    182945-plasmid-map-sequence-id-360103

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  18. pShuttle-Flag-HA

    Plasmid
    #63681
    Purpose
    adenoviral shuttle vector containing N-terminal Flag tag
    Depositor
    Lingfang Zeng, Qingbo Xu
    Type
    Empty backbone
    Use
    Adenoviral
    Tags
    FLAG and HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    63681-plasmid-map-sequence-id-263358

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  19. pANY4

    Plasmid
    #164897
    Purpose
    Two valuable constructs for either IPTG-induced or temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4.
    Depositor
    Yingfeng An
    Insert
    ccdB cassette
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    164897-plasmid-map-sequence-id-327986

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  20. pANY8

    Plasmid
    #164900
    Purpose
    Containing His-ELP-intein multifunctional tag for protein purification by combining Ni-NTA-based and ELP-based strategy with a specially designed purification protocol.
    Depositor
    Yingfeng An
    Insert
    ccdB cassette
    Use
    Tags
    His-ELP-Intein-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    164900-plasmid-map-sequence-id-330483

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  21. pANY4-pL18-ccdB

    Plasmid
    #164898
    Purpose
    Two valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pL18-ccdB.
    Depositor
    Yingfeng An
    Insert
    ccdB cassette
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    164898-plasmid-map-sequence-id-327989

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  22. pANY4-pR18/pL18-ccdB

    Plasmid
    #164899
    Purpose
    Two valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pR18/pL18-ccdB.
    Depositor
    Yingfeng An
    Insert
    ccdB cassette
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    164899-plasmid-map-sequence-id-327990

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  24. p75 NTR

    Plasmid
    #24091
    Depositor
    Moses Chao
    Insert
    p75NTR (Ngfr Rat)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 2, 2013
    Availability
    Academic Institutions and Nonprofits only

  25. mEgr1/LZRS

    Plasmid
    #27783
    Depositor
    David Wiest
    Insert
    Egr1 (Egr1 Mouse)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 18, 2011
    Availability
    Academic Institutions and Nonprofits only

  30. Hygro-iDEST

    Plasmid
    #75339
    Purpose
    GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression
    Depositor
    Danwei Huangfu
    Article
    Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-
    Insert
    TRE-DEST
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE-tight
    Available since
    May 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    75339-plasmid-map-sequence-id-142552

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  31. Puro-iDEST

    Plasmid
    #75336
    Purpose
    GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression
    Depositor
    Danwei Huangfu
    Article
    Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-
    Insert
    TRE-DEST
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE-tight
    Available since
    June 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    75336-plasmid-map-sequence-id-149881

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  38. EGR-1 TurboRFP

    Plasmid
    #52306
    Purpose
    Fluid Shear Stress inducible Red Fluorescent Protein for mammalian expression
    Depositor
    Joel Voldman
    Article
    Varma et al Lab Chip. 2015 Feb 4.
    Insert
    EGR-1 Promoter (Egr1 Mouse)
    Use
    Tags
    TurboRFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    52306-plasmid-map-sequence-id-322920

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Showing: 361 - 400 of 595 results