173,361 results
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Plasmid#186789PurposeNAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the nucleusDepositorInsertnuclear NAD+ biosensor
UseLentiviralExpressionMammalianAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKH144_mCherry-YIPF4 WT
Plasmid#214972PurposemCherry tagged YIPF4DepositorInsertmCherry-YIPF4 (YIPF4 Human)
UseLentiviralAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-yZ3EV-Z3pr-yEGFP (RB3579)
Plasmid#69100PurposeURA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3pr driving expression of yEGFPDepositorInsertsyZ3EV cassette
Z3 promoter
yEGFP
ExpressionYeastAvailable SinceSept. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas13-M3nls
Plasmid#155366PurposeTargeted m6A RNA methylation in mammalian cellsDepositorInsertdCas13-M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
UseCRISPRExpressionMammalianMutationPspCas13b Δ984-1090 H133AAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-CD19.8H.8TM.28.BB.3z
Plasmid#200672PurposeModular CD8hinge-CD8transmembrane-CD28-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsMycTagExpressionMammalianPromoterEF1a-shortAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
rBiFC Kit
Plasmid Kit#1000000147PurposePlasmids for analysis of protein-protein interactions in planta via ratiometric Bimolecular Fluorescence Complementation (rBiFC).DepositorApplicationCloning and Synthetic Biology, Gene Expression and LabelingVector TypePlant ExpressionCloning TypeGatewayAvailable SinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-GFP (AAV8)
Viral Prep#37825-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsGFPAvailable SinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
YY203: pAAV_cfos::mCherry-GEMINI(A)-P1N4
Plasmid#228885PurposeAAV plasmid expressing the destabilized mCherry-GEMINI(A) under the control of a cfos promotor.DepositorInsertmCherry-GEMINI(A)
UseAAVTagsmCHERRYExpressionMammalianPromotercfosAvailable SinceFeb. 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
YY225: pAAV_TRE::GFP- GEMINI(A)-P1N4
Plasmid#228888PurposeAAV plasmid expressing the destabilized GFP-GEMINI(A) under the control of a doxycycline-inducible promotor.DepositorInsertGFP-GEMINI(A)
UseAAVTagsgfpPromoterTREAvailable SinceFeb. 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-FIRE-pHLy
Plasmid#170774PurposeRatiometric biosensor expressed with UbC promoter for probing lysosomal pH in mammalian cellsDepositorInsertLAMP1 (LAMP1 Human)
UseLentiviralTagsmCherry and mTFP1ExpressionMammalianMutation84 nucleotide Signal Sequence switched to the beg…PromoterhUbCAvailable SinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
NanoBRET Assay Vector, CRAF(FL)-HaloTag
Plasmid#236874PurposeExpress CRAF(FL)-HaloTag(R) in Mammalian Cells under a CMV promoterDepositorHas ServiceDNAInsertCRAF (RAF1 Human)
TagsHaloTag (R)ExpressionMammalianMutationNonePromoterCMVAvailable SinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Tn7 integration plasmid
Plasmid#154134PurposeSuicidal integration plasmid with the Tn7 arms, streptomycin and kanamycin resistance genes and the restriction site to clone the barcode-carrying cassette.DepositorInsertsleft and right end of Tn7 arm
Spectinomycin resistant gene
ExpressionBacterialPromoterSpectinomycin resistance gene promoter and noAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
p37-2iDMD-LR
Plasmid#88892Purpose37°C full-length DMD luc-red plasmid. Co-expresses human dystrophin, firefly luciferase and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites and can be grown at 37°C.DepositorInsertsExpressionMammalianAvailable SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-MAT2A
Plasmid#100521PurposeDox-repressible cDNA expression of MAT2A (dox-off/ tet-off all-in-one vector)DepositorAvailable SinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
14UAS PSD95:GFP 5UAS DSRedExpress
Plasmid#74315PurposeEncodes a PSD95:GFP fusion protein and DsRedExpress from separate UAS cassettes; for long-term two-photon time-lapse imaging of dendrite growth and synaptogenesis in zebrafishDepositorInsertszebrafish PSD95
DsRedExpress
TagsEGFPMutationY599H and I793S (please see depositor comment bel…Available SinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD-Lime
Plasmid#191255PurposeBacterial expression plasmids for pH sensitive fluorescent protein LimeDepositorInsertLime
TagsHis tagExpressionBacterialAvailable SinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-sadCas9-VP64
Plasmid#115790PurposeAn AAV vector expressing S. aureus dCas9 fused to VP64.DepositorInsertSa-dCas9
UseAAVTagsNLS and NLS-VP64ExpressionMammalianPromoterCMVAvailable SinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-cytoEKAR4
Plasmid#174438PurposeCytosol-targeted EKAR4.DepositorInsertcytoEKAR4
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-2E2-HA-mEGFP
Plasmid#129595PurposeThe encoded protein is the anti-HA frankenbody variant fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.DepositorInsertAnti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
ExpressionMammalianPromoterCMVAvailable SinceAug. 15, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJH4783
Plasmid#200785PurposePnpr-4 snb-1::pHluorin unc-54 3' UTR C.elegans AVA and other neurons expression of snb pHluorinDepositorAvailable SinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLJC6-2XFLAG-TMEM192
Plasmid#104435PurposeExpresses 2XFLAG-TMEM192 in mammalian cellsDepositorAvailable SinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-APOBEC1-YTH
Plasmid#200158PurposeFor bacterial expression of the APOBEC1-YTH fusion protein for protein purificationDepositorInsertAPOBEC1-YTH
Tags6xHis, HA, and Maltose Binding ProteinExpressionBacterialMutationCodon optimized for E coli expressionPromoterLacZAvailable SinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGLuc
Plasmid#135288PurposeGBPart - GLuc Luciferase CDSDepositorInsertGaussia Luciferase
UseLuciferase and Synthetic BiologyAvailable SinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
SP-Cas9
Plasmid#62731PurposeExpresses SP-Cas9 protein in bacterial cellsDepositorInsertSP-CAS9
UseCRISPRTagsHis tag and NLSExpressionBacterialMutationCodon optimazed for e.coliPromoterT7Available SinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
TALED_Left-ND1-1397C-AD
Plasmid#183892PurposeExpresses TALED_Left-ND1-1397C-AD in mammalian cellsDepositorInsertTALED for ND1 mutation
ExpressionMammalianPromoterpCMVAvailable SinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
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Human GeCKO Lentiviral sgRNA Library v2 (lentiGuide-Puro)
Pooled Library#1000000049PurposeCRISPR gRNA knockout pooled library for targeting the human genomeDepositorAvailable SinceMay 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-NES-jRCaMP1a
Plasmid#61562PurposeRed fluorescent calcium sensor proteinDepositorInsertNES-jRCaMP1a
Tagsnuclear export signalExpressionMammalianPromoterCMVAvailable SinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR
Plasmid#126705Purposedonor vector for targeting a 2A peptide followed by green fluorescent reporter to the human ACTA2 locus at the end of the endogenous coding sequenceDepositorInsertGFP
UseCRISPRAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tol2_ISCro4_Insertion_Reporter
Plasmid#251500PurposeReporter plasmid for monitoring insertion activity of ISCro4 bridge recombinase in the presence of native bridge RNA or native TBL and DBL and pDonor_ISCro4_(WT)_Donor_ECFP plasmidDepositorInsertISCro4 insertion reporter
ExpressionMammalianMutationNAAvailable SinceFeb. 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
pOpen-RNAse H
Plasmid#165575PurposeEndonuclease that specifically degrades the RNA of RNA-DNA hybrids. Participates in DNA replication; helps to specify the origin of genomic replication.DepositorInsertRNAse H
UseSynthetic BiologyAvailable SinceAug. 24, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
7TFC
Plasmid#24307DepositorInsert7xTcf-FFluc // SV40-mCherry
UseLentiviralAvailable SinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
TALED_Right-ND1-1397N
Plasmid#183898PurposeExpresses TALED_Right-ND1-1397N in mammalian cellsDepositorInsertTALED for ND1 mutation
ExpressionMammalianPromoterpCMVAvailable SinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Eco
Plasmid#35617DepositorInsertEcotropic MLV envelope
ExpressionMammalianAvailable SinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUPD2
Plasmid#68161PurposeUniversal domesticator plasmid for level 0 partsDepositorInsertempty vector
UseSynthetic Biology; Domestication of dna parts for…Available SinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Wnt7A
Plasmid#35914DepositorAvailable SinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
lenti_dCas9-KRAB-MeCP2
Plasmid#122205PurposeBipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.DepositorInsertdCas9-KRAB-MeCP2
UseLentiviralTagsKRAB-MeCP2ExpressionMammalianMutationCas9 (D10A, H840A)Available SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET15b-His-RF2
Plasmid#124130PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP‐RNASEH2A
Plasmid#108700PurposeFor expression of N-terminally eGFP-tagged human RNASEH2A in mammalian cellsDepositorAvailable SinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCW-HA-hRb-WT-puro
Plasmid#212672PurposeExpress tagged hRbDepositorAvailable SinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only