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SF-1-A172S

Plasmid

#28185

Depositor

Bernard Schimmer

Article

Frigeri et al Endocrinology. 2002 Oct . 143(10):4031-7

Insert

SF-1 (Nr5a1 Mouse)

Expression

Mammalian

Mutation

A172S (polymorphism)

Available Since

May 12, 2011

Availability

Academic Institutions and Nonprofits only
pHES942

Plasmid

#90408

Purpose

Expresses SIC1 udner control of synthetic promoter containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pZ-SIC1

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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pRSV-Rev

Plasmid

#12253

Purpose

3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)

Depositor

Didier Trono

Article

Dull et al J Virol. 1998 Nov . 72(11):8463-71.

Insert

Rev (rev )

Use

Lentiviral; Packaging

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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Olig001

Plasmid

#170716

Purpose

Olig001 encodes a novel AAV capsid that exhibits preferential oligodendrocyte tropism in the CNS

Depositor

Thomas McCown

Article

Powell et al Gene Ther. 2016 Nov;23(11):807-814. doi:

Insert

Olig001 capsid gene / rep 2

Use

AAV

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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EF06R

Plasmid

#42940

Depositor

Eline Luning Prak

Article

Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

Insert

Human L1 element (L1RE1 Human)

Tags

EGFP (opposite strand)

Expression

Mammalian

Promoter

CMV MIE, L1 5'UTR

Available Since

April 25, 2013

Availability

Academic Institutions and Nonprofits only

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TATA-GFP

Plasmid

#110833

Purpose

negative control

Depositor

Michael Lewis

Article

Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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myc-NLRC5

Plasmid

#37509

Depositor

Thomas Kufer

Article

Neerincx et al J Immunol. 2012 May 15;188(10):4940-50.

Insert

NLRC5 (NLRC5 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Dendra2

Plasmid

#103967

Purpose

Mammalian expression of fluorescent protein Dendra2 used for transfection efficiency control

Depositor

Qing Deng

Article

Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

Insert

Dendra2

Expression

Mammalian

Available Since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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RCASBP-AP (A) (CC#37)

Plasmid

#15160

Depositor

Connie Cepko

Article

Fekete et al Mol Cell Biol. 1993 Apr . 13(4):2604-13.

Insert

PLAP (ALPP Human)

Use

Retroviral; Avian expression

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only

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pLOX-Ttag-iresTK

Plasmid

#12246

Depositor

Didier Trono

Article

Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

Insert

SV40 Large T, HSV1-TK (SV40gp6 Simian virus)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

LoxP in 3' LTR.

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pIVT-tagBFP_pKG03832

Plasmid

#246370

Purpose

IVT template for making modRNA encoding tagBFP

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

tagBFP

Use

Synthetic Biology; In vitro transcription

Expression

Mammalian

Mutation

none

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-GRAB_ATP1.0

Plasmid

#167579

Purpose

Express the ATP sensor GRAB_ATP1.0 in astrocytes

Depositor

Yulong Li

Article

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP sensor GRAB_ATP1.0 (P2RY1 Human)

Use

AAV

Promoter

GfaABC1D

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-neo-cOVA

Plasmid

#25097

Depositor

Maria Castro

Article

Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

Insert

cytoplasmic ovalbumin

Expression

Bacterial and Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pHB-4

Plasmid

#140957

Purpose

For PHB production in E. coli (thrC3-pAC). Please note that this is listed as #140956 in the publication.

Depositor

Kang Zhou

Article

Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

Insert

phaCAB

Expression

Bacterial

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJB127

Plasmid

#248630

Purpose

Theophylline-based control of repA on a C. difficile plasmid for use in allelic exchange and codA added to help cure strains of the plasmid.

Depositor

Joseph Sorg

Article

Brehm et al Anaerobe. 2024 Apr 29;88:102858. doi: 10

Inserts

sacB (sacB )
codA

Use

Other

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pSK9065

Plasmid

#84345

Purpose

Tightly controlled expression of mRFP fusion proteins in S. aureus

Depositor

Neville Firth

Article

Brzoska et al Appl Environ Microbiol. 2013 May;79(9):3

Type

Empty backbone

Tags

mRFPmars

Expression

Bacterial

Promoter

Pxyl/tet

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-cdc2 (716)

Plasmid

#61840

Purpose

mammalian expression of cdc2

Depositor

Jonathon Pines

Article

Hagting et al EMBO J. 1998 Jul 15;17(14):4127-38.

Insert

cdc2 (CDK1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pHR_CMV_MeltNLS/NES-40-mCherry

Plasmid

#234066

Purpose

Encodes a protein that localizes to nucleus under the control of a Melt variant with a switching temperature of 40°C

Depositor

Lukasz Bugaj

Article

Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

Insert

MeltNLS/NES-40-mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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Ac5-STABLE2-neo

Plasmid

#32426

Depositor

Rosa Barrio, James Sutherland

Article

Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

Insert

Flag-mCherry-T2A-GFP-T2A-neo

Expression

Insect

Promoter

Actin5C

Available Since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pHaMDRwt

Plasmid

#10957

Depositor

Michael Gottesman

Article

Pastan et al Proc Natl Acad Sci U S A. 1988 Jun . 85(

Insert

MDR1 (ABCB1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

March 14, 2006

Availability

Academic Institutions and Nonprofits only

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pCS2-3xFLAG-NLS-SpCas9-NLS

Plasmid

#51307

Purpose

Cas9 expression plasmid

Depositor

Yonglong Chen

Article

Guo et al Development. 2014 Jan 8.

Insert

3xFLAG-NLS-SpCas9-NLS

Use

CRISPR; Xenopus tropicalis expression

Tags

3x Flag and NLS

Promoter

CMV

Available Since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-3xFLAG-TEV-UBE2O

Plasmid

#105718

Purpose

Expresses WT full length human N- terminally tagged UBE2O

Depositor

Ramanujan Hegde

Article

Yanagitani et al Science. 2017 Aug 4;357(6350):472-475. d

Insert

UBE2O (UBE2O Human)

Tags

3xFLAG-TEV

Expression

Mammalian

Promoter

CMV (from vector)

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCIG3 N

Plasmid

#132941

Purpose

MLV packaging plasmid for N-tropic MLV gag-pol expression

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

N-tropic MLV gag-pol

Use

Retroviral

Available Since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pCIG3 B

Plasmid

#132942

Purpose

MLV packaging plasmid for B-tropic MLV gag-pol expression

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

B-tropic MLV gag-pol

Use

Retroviral

Available Since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-gfaABC1D-mCherry-CAAX

Plasmid

#218189

Purpose

To over express mCherry-CAAX under gfaABC1D promoter in astrocytes

Depositor

Cagla Eroglu

Article

Bindu et al eLife. 2024, Reviewed Preprint. 13:RP103

Insert

mCherry-CAAX

Use

AAV

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCW01

Plasmid

#72300

Purpose

Starting substrate for in vitro DNA mismatch repair substrate monitored by PstI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Type

Empty backbone

Use

Derivative of puc19

Expression

Bacterial

Promoter

lac

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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sgTelo

Plasmid

#227392

Purpose

Adhesion module, guide RNA recognizes telomeres

Depositor

Clifford Brangwynne

Article

Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

Insert

guide RNA targeting telomeres

Use

Lentiviral

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-neo-sOVA

Plasmid

#25098

Depositor

Maria Castro

Article

Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

Insert

soluble ovalbumin

Expression

Bacterial and Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV_Cas9_puro

Plasmid

#65655

Purpose

Retroviral introduction of Cas9 into mammalian cell line.

Depositor

Christopher Vakoc

Article

Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

Insert

Cas9

Use

CRISPR and Retroviral

Tags

5 ' FLAG tag

Expression

Mammalian

Promoter

MSSV_LTR

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pLys-M1

Plasmid

#109382

Purpose

Arabinose inducible VioB-mCherry expression (stronger RBS). Used to test burden response in E. coli

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pARABAD-VioB-mCherry

Expression

Bacterial

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVCT-tTR-KRAB

Plasmid

#11643

Purpose

Tet-regulated (Tet-on) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

CAG promoters, GFP, tTR-KRAB, Tet-on

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2_170

Plasmid

#199102

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pSa ORI plus NGS barcode 16, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pIVEX-SNAP-GFP

Plasmid

#46846

Purpose

SNAP-GFP fusion

Depositor

Florian Hollfelder

Article

Diamante et al Protein Eng Des Sel. 2013 Oct;26(10):713

Insert

SNAP-GFP fusion

Use

In vitro expression

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEMT-PTE2A

Plasmid

#111773

Purpose

Quadcistronic construct: Co-express four genes by three 2A peptides -- P2A, T2A and E2A

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Type

Empty backbone

Use

Cloning intermediate

Promoter

T7

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
H2B-GFP-AsLOV2-NES27 (pDN158)

Plasmid

#72659

Purpose

Constitutively nuclear variant of a very strong, photocaged NES (AsLOV2-NES 27) for blocking endogenous, CRM-1-dependent nuclear export

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Insert

H2B-GFP-AsLOV2-NES27

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_nMagHigh1-EGFP-CAAX

Plasmid

#67300

Purpose

Blue light dependent photoswitching system

Depositor

Moritoshi Sato

Article

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

nMagHigh1-EGFP-CAAX

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 ZoAl N-1F

Plasmid

#203763

Purpose

For expression of N-flag tagged ZoAl R2 protein in mammalian cells

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

R2 retroelement protein

Tags

Flag

Expression

Mammalian

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRX-IP TFEB-sfGFP

Plasmid

#135402

Purpose

Expresses TFEB-sfGFP

Depositor

Eisuke Itakura

Article

Ishii et al Sci Rep. 2019 Aug 12;9(1):11635. doi: 10

Insert

TFEB (TFEB Human)

Use

Retroviral

Tags

sfGFP

Expression

Mammalian

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-eSIBR

Plasmid

#71665

Purpose

intronically embedded eSIBR backbone in gateway middle entry vector (pDONR221)

Depositor

Philip Washbourne

Article

Fowler et al Nucleic Acids Res. 2015 Nov 17. pii: gkv

Insert

intronic eSIBR cassette

Use

RNAi; Multi-site gateway

Promoter

None

Available Since

Feb. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA-MAVS-M142A(KaganF61)

Plasmid

#51999

Purpose

Expresses the human MAVS CDS containing the point mutation M142A and an N-terminal HA tag "strong translational context"

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS

Tags

HA

Expression

Mammalian

Mutation

Met 142 to Ala

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

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