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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,323 results for: Coli

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Showing: 4121 - 4160 of 10323 results

  3. pKB238

    Plasmid
    #170033
    Purpose
    Encodes LacI-controlled expression of surface-displayed NPY and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    Neuropeptide Y (NPY Human, Escherichia coli, Synthetic)
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Available Since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    170033-plasmid-map-sequence-id-336781

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  4. pET21-Flavidin

    Plasmid
    #89881
    Purpose
    Fluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labeling
    Depositor
    Mark Howarth
    Article
    Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-
    Insert
    Streptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
    Tags
    6 glutamate tag (C terminal on insert)
    Expression
    Bacterial
    Mutation
    K80R, K121R, K132R, K134R
    Available Since
    Aug. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    89881-plasmid-map-sequence-id-177281

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  6. pBP-J23100

    Plasmid
    #72963
    Purpose
    Level 0 - J23100 standard iGEM promoter (5' CTAT/3' GTAC fusion)
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    J23100 promoter
    Use
    Synthetic Biology
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72963-plasmid-map-sequence-id-133808

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  7. pBP-lacZ

    Plasmid
    #72948
    Purpose
    Entry vector for Bioparts - Oligo anneal or PCR with internal BsaI sites at 5' and 3' ends using NdeI/SphI cloning
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Level 0 - Bioparts
    Use
    Synthetic Biology
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72948-plasmid-map-sequence-id-133793

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  9. pCNA

    Plasmid
    #135191
    Purpose
    Arabinose inducible expression of lamba-Red recombinase proteins and I-CreI.
    Depositor
    Hongqing Fang
    Article
    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d
    Insert
    I-CreI lamba Red
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    None
    Promoter
    araBAD
    Available Since
    March 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    135191-plasmid-map-sequence-id-273788

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  12. pSNK

    Plasmid
    #135193
    Purpose
    Arabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains kamamycin resistance cassette
    Depositor
    Hongqing Fang
    Article
    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d
    Insert
    I-SceI lamba Red
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    None
    Promoter
    araBAD
    Available Since
    April 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    135193-plasmid-map-sequence-id-288121

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  13. pDR111_mannose_Bgal NLS

    Plasmid
    #188398
    Purpose
    Expresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.
    Depositor
    Christopher Contag
    Article
    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:
    Insert
    lacZ
    Tags
    PhoD secretion peptide and SV40 NLS
    Expression
    Bacterial
    Promoter
    D-mannose
    Available Since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188398-plasmid-map-sequence-id-370473

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  17. pCRIS

    Plasmid
    #120424
    Purpose
    This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3, IS5, and IS150.
    Depositor
    Tamás Fehér
    Article
    Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:
    Insert
    CRISPR spacers targeting IS1, IS5, IS3, IS150
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    constitutive
    Available Since
    Jan. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    120424-plasmid-map-sequence-id-233246

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  21. pNAS1B

    Plasmid
    #61968
    Purpose
    Duet vector for co-expression of GFP and mCherry from PBAD and PLtetO promoters, respectively; parent vector for split mCherry assembly vectors
    Depositor
    Lynne Regan
    Article
    Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.
    Inserts
    mCherry
    GFP
    Expression
    Bacterial
    Mutation
    Serine 65 to Glycine (GGT), Serine 72 to Alanine …
    Promoter
    PBAD and PLtetO
    Available Since
    Jan. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    61968-plasmid-map-sequence-id-101267

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  22. pTU2S-a (p15A origin)

    Plasmid
    #74088
    Purpose
    Secondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replication
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Golden Gate destination vector - Level 2, 2 TU's with secondary module and p15A origin
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    None
    Available Since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    74088-plasmid-map-sequence-id-138296

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Showing: 4121 - 4160 of 10323 results