We narrowed to 4,529 results for: GCA

1. Helios-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

   Plasmid

   #75247

   Purpose

   CRISPR/Cas9 NICKASE plasmid against human Helios (2/2)

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against human Helios (IKZF2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1 mTagBFP2 rat PGK1 shRNA

   Plasmid

   #222869

   Purpose

   Expresses mTagBFP2 along with an shRNA against rat PGK1

   Depositor

   Timothy Ryan

   Article

   Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

   Insert

   PGK1 shRNA (Pgk1, Pgk1 Mouse, Rat)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CMV-FLEX-SaCas9-U6-sgCnr1

   Plasmid

   #209196

   Purpose

   Mutagenesis of Cnr1

   Depositor

   Larry Zweifel

   Article

   Gunduz-Cinar et al Neuron. 2023 Jul 19:S0896-6273(23)00482-

   Insert

   Cnr1 (Cnr1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2

   Plasmid

   #217635

   Purpose

   AAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.

   Depositor

   Martin Kampmann

   Article

   Ramani et al Nat Neurosci. 2025 Aug 22. doi: 10.1038/

   Insert

   mU6-sgRNA, Handle sequence, EF1a-mTagBFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1alpha and mU6

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Px330-EGFP-LRRK2-CRISPR/Cas9

   Plasmid

   #180437

   Purpose

   Plasmid to express Cas9 from S.pyogenesand CRISPR gRNA to introduce LRRK2-G2019S mutation in human cells

   Depositor

   Frank Soldner

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   gRNA targeting LRRK2-G2019S (LRRK2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. sgAPC(B)

   Plasmid

   #85558

   Purpose

   Expresses sgRNA targeting human APC around codon I1417

   Depositor

   Luca Grumolato

   Article

   Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

   Insert

   APC, WNT signaling pathway regulator (APC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

7. pORANGE GFP-Grin1 KI

   Plasmid

   #131485

   Purpose

   Endogenous tagging of GluN1: N-terminal (amino acid position: A20)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Grin1, Grin1 Mouse, Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBoBi-hLAMP2-C-GC6s

   Plasmid

   #154151

   Purpose

   To detect the calcium ion releases close to the lysosomal surface

   Depositor

   Sheng-cai Lin

   Article

   Li et al Cell Metab. 2019 Sep 3;30(3):508-524.e12

   Insert

   LAMP2 and GCamp6s (LAMP2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   CMV

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pT7NT*-Bri2 113-231 R221E

   Plasmid

   #138134

   Purpose

   Expresseds human Bri2 BRICHOS R221E mutant in E. coli

   Depositor

   Jan Johansson

   Article

   Chen et al Commun Biol. 2020 Jan 20;3(1):32. doi: 1

   Insert

   human Bri2 BRICHOS R221E (ITM2B Human)
   

   Use

   Tags

   NT* tag derived from spider silk proteins

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. sgTP53_3

    Plasmid

    #78164

    Purpose

    sgTP53

    Depositor

    William Hahn

    Article

    Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

    Insert

    TP53 (TP53 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pLentiCMVblast_SLC38A2_wt

    Plasmid

    #156180

    Purpose

    CMV-driven expression of sgRNA-resistant SLC38A2 wild-type cDNA.

    Depositor

    Alec Kimmelman

    Article

    Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

    Insert

    SLC38A2 (SLC38A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Silent mutations to prevent targeting by sgRNA se…

    Promoter

    CMV

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pORANGE GFP-Actb KI

    Plasmid

    #131479

    Purpose

    Endogenous tagging of βactin: N-terminal (amino acid position: D2)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Actb, Actb Mouse, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Ad:2CMV_A2R2-N25Ctr

    Plasmid

    #122243

    Purpose

    Expresses a dominant negative Kash control, that lacks the actual Kash domain for LINC complex integration, in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone

    Depositor

    Corey Neu

    Article

    Seelbinder et al Nat Biomed Eng. 2021 Dec;5(12):1500-1516

    Inserts

    C-terminal end of Nesprin-3 w/o Kash (Syne3 Mouse)
    Actinin alpha 2 (Actn2 Mouse)
    

    Use

    Adenoviral

    Tags

    Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…

    Expression

    Mammalian

    Mutation

    deleted Kash domain (GGCGAGGAGGAGCGCAGCTGCGCCCTGG…

    Promoter

    CMV

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOTTC763 - pX458 with rat Rosa26 gRNA A

    Plasmid

    #113161

    Purpose

    A plasmid that expresses a guide RNA targeting rat Rosa26 as well as FLAG-tagged SpCas9 and GFP

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    gRNA for rat Rosa26
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKLV2-U6gRNA5(LIN28A_5-4)-PGKpuroBFP-W

    Plasmid

    #211969

    Purpose

    Express gRNA against LIN28A with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting LIN28A (LIN28A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-mTubb3

    Plasmid

    #87116

    Purpose

    AAV backbone plasmid including GFP knock-in donor and Tubb3gRNA for HITI

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    U6-mTubb3sgRNA-GFP-EF1a-mCherryKASH-hGHpA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MAGI1 gRNA (BRDN0001146934)

    Plasmid

    #78069

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAGI1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAGI1 (MAGI1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ATM gRNA (BRDN0001149518)

    Plasmid

    #77529

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ATM

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ATM (ATM Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMXS-IRES-BLAST EGFP-flag-CAD

    Plasmid

    #188118

    Purpose

    Expresses N-terminal EGFP-tagged/C-terminal flag-tagged human CAD in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    EGFP tag and Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-GFAP-eGFP-shAqp4-1

    Plasmid

    #223223

    Purpose

    mir30 based shRNA strategy, shRNA targeting mouse Aqp4

    Depositor

    Li-Huei Tsai

    Article

    Murdock et al Nature. 2024 Mar;627(8002):149-156. doi:

    Insert

    shAqp4 (Aqp4 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. sgTP53(A)

    Plasmid

    #85559

    Purpose

    Expresses sgRNA targeting human TP53 around codon F109

    Depositor

    Luca Grumolato

    Article

    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

    Insert

    tumor protein p53 (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

22. pKLV2-U6gRNA5(hYAP1-Y2B)-PGKpuro2AmCherry-W

    Plasmid

    #163179

    Purpose

    Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of mCherry tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

    Insert

    YAP1 (YAP1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pORANGE GFP-CACNA1E KI

    Plasmid

    #131481

    Purpose

    Endogenous tagging of CaV2.3, R: N-terminal (amino acid position: G5)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Cacna1e, Cacna1e Mouse, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. sgTP53(B)

    Plasmid

    #85560

    Purpose

    Expresses sgRNA targeting human TP53 around codon F109

    Depositor

    Luca Grumolato

    Article

    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

    Insert

    tumor protein p53 (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

25. pRSITEP-puro-shWRN1

    Plasmid

    #125783

    Purpose

    DOX-inducible expression of a short-hairpin RNA targeting human WRN

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    shWRN1 (WRN Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pXPR003-sgTP53-3

    Plasmid

    #118021

    Purpose

    Constitutive expression of sgRNA targeting human TP53

    Depositor

    William Hahn, David Root

    Article

    Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

    Insert

    sgTP53-3 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMXS-IRES-BLAST Halo-flag-CAD

    Plasmid

    #188117

    Purpose

    Expresses N-terminal Halo-tagged/C-terminal flag-tagged human CAD in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag and Halo tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PRKDC gRNA (BRDN0001149021)

    Plasmid

    #77864

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKDC

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKDC (PRKDC Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDONR223-TGFBR3

    Plasmid

    #23478

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    TGFBR3 (TGFBR3 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    C44T, 1MM

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

30. pRSITEP-puro-shWRN1-C911

    Plasmid

    #125784

    Purpose

    a seed-matched control for pRSITEP-puro-shWRN1

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    shWRN1-C911 (WRN Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pXPR003-sgTP53-5

    Plasmid

    #118023

    Purpose

    Constitutive expression of sgRNA targeting human TP53

    Depositor

    William Hahn, David Root

    Article

    Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

    Insert

    sgTP53-5 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pKLV2-U6gRNA5(SOX2_v32_7-4)-PGKpuroBFP-W

    Plasmid

    #211986

    Purpose

    Express gRNA against SOX2 with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting SOX2 (SOX2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLKO.1-puro-shWRN1

    Plasmid

    #125781

    Purpose

    constitutive expression of a short-hairpin RNA targeting human WRN

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    shWRN1 (WRN Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. sgEGFR(A)

    Plasmid

    #85564

    Purpose

    Expresses sgRNA targeting human EGFR around codon T790

    Depositor

    Luca Grumolato

    Article

    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

    Insert

    epidermal growth factor receptor (EGFR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

35. Lenti-sgTp53#2/Cre

    Plasmid

    #173621

    Purpose

    Expresses a Tp53-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Tp53 (Trp53 Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. sgCRY1

    Plasmid

    #176511

    Purpose

    Expresses sgRNA in mammalian cells

    Depositor

    Han Kyoung Choe

    Article

    Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

    Insert

    hU6-sgCRY1-2 (Cry1, Cry2 Mouse)
    

    Use

    AAV

    Tags

    mCherry (K14E, N210D, E249G)

    Expression

    Mutation

    Promoter

    U6, hSyn

    Available since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. FGFR1 gRNA (BRDN0001145494)

    Plasmid

    #76067

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human FGFR1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    FGFR1 (FGFR1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. AKT3 gRNA (BRDN0001147841)

    Plasmid

    #76220

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human AKT3

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    AKT3 (AKT3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMXS-IRES-BLAST flag-CAD E1368A

    Plasmid

    #188141

    Purpose

    Expresses C-terminal flag-tagged human CAD E1368A in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pInducer20_SLC38A2_wt

    Plasmid

    #156182

    Purpose

    Dox-inducible expression of sgRNA-resistant SLC38A2 wild-type cDNA.

    Depositor

    Alec Kimmelman

    Article

    Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

    Insert

    SLC38A2 (SLC38A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Silent mutations to prevent targeting by sgRNA: T…

    Promoter

    TRE

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge