We narrowed to 34,796 results for: cas

1. RCAS (A) Six1 (CT#664)

   Plasmid

   #13876

   Depositor

   Cliff Tabin

   Article

   Heanue et al Genes Dev. 1999 Dec 15. 13(24):3231-43.

   Insert

   Six1 (Six1 Mouse)
   

   Use

   Retroviral; Avian expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

2. Lenti Cas9/SB100

   Plasmid

   #155294

   Purpose

   Lentiviral expression of Cas9 and SB100

   Depositor

   Martin Carroll

   Insert

   SB100X-P2A-spCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACYC-Cas12c2-noncoding

   Plasmid

   #120877

   Purpose

   Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12c2 system in the native genomic loci; expresses the tracrRNA required for activity.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Noncoding sequences for CRISPR-Cas12c2
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221 Cas9-RFC5

   Plasmid

   #183200

   Purpose

   Entry clone

   Depositor

   Emma Haapaniemi

   Article

   Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

   Insert

   Cas9-RFC5
   

   Use

   CRISPR; Gateway entry clone

   Tags

   Expression

   Mutation

   n/a

   Promoter

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAT15546_nABE8e-SuperFi-Cas9

   Plasmid

   #184376

   Purpose

   Mammalian expression of SuperFi-Cas9 ABE8e base editor

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

   Insert

   nABE8e-SuperFi-Cas9
   

   Use

   CRISPR

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…

   Promoter

   EF-1α core promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9_BB_2A-GFP_MAPRE1-gRNA#2

   Plasmid

   #107727

   Purpose

   Knock out of EB1 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-TAC-OKMS-Cas9

   Plasmid

   #120353

   Purpose

   piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Insert

   OKMS cassette
   

   Use

   CRISPR; Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Klf4 [10-483]

   Promoter

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-LmoCas6-p300

   Plasmid

   #126486

   Purpose

   Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   LmoCas6-p300
   

   Use

   CRISPR

   Tags

   Flag and Flag, NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEJS1026-pMCSG7-HpaCas9

   Plasmid

   #121540

   Purpose

   Expresses a 6X-His tagged type II-C Cas9 from H. parainfluenzae in bacterial cells

   Depositor

   Erik Sontheimer

   Article

   Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

   Insert

   HpaCas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAC92-pmax-dCas9VP96

    Plasmid

    #48224

    Purpose

    dCas9VP96 on pmax expression vector

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP96 activation domain
    

    Use

    CRISPR

    Tags

    HA Tag and VP96

    Expression

    Mammalian

    Mutation

    D10A;H840A

    Promoter

    CAGGS

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. ciCas9(L22)_pcDNA5

    Plasmid

    #100551

    Purpose

    Expresses ciCas9(L22) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

    Insert

    ciCas9(L22)
    

    Use

    CRISPR; Frt, flp-in

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEJS1027-pMCSG7-SmuCas9

    Plasmid

    #121541

    Purpose

    Expresses a 6X-His tagged type II-C Cas9 from S. muelleri in bacterial cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Insert

    SmuCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFREE2-xCas9-sgRNA

    Plasmid

    #179579

    Purpose

    Expresses xCas9 and gRNA that targets ColE1 plasmids for curing.

    Depositor

    Kevin Solomon

    Article

    Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

    Insert

    xCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. PX458-3xHA-SpCas9

    Plasmid

    #130968

    Purpose

    3xHA tagged Cas9 from S. pyogenes with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

    Insert

    3xHA-NLS-SpCas9
    

    Use

    CRISPR

    Tags

    3x HA, EGFP, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. 6xHis-MBP-huLb5Cas12a

    Plasmid

    #174684

    Purpose

    Expresses 6xHis-MBP tagged Lb5Cas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huLb5Cas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. LbCas12a-DR-GC-swap-cr in pBCSK+

    Plasmid

    #126643

    Purpose

    Cloning vector LbCas12a-DR-GC-swap-crRNA for expression in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    LbCas12a Full Length Direct Repeat GC Swap crRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST-APIC-Cas9

    Plasmid

    #121657

    Purpose

    gateway cloning destination vector for expressing enhancer driven Cas9

    Depositor

    Chun Han

    Article

    Poe et al Genetics. 2018 Nov 30. pii: genetics.118

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. A3D-Cas9n-UGI

    Plasmid

    #119137

    Purpose

    Base editor made from APOBEC3D

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    APOBEC3D-Cas9 nickase-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    None

    Promoter

    CMV

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRS415-Cas9-VPR

    Plasmid

    #163971

    Purpose

    Trifunctional Cas9-VPR fusion protein plasmid for simultaneous transcriptional activation, transcriptional repression, and genome editing in yeast

    Depositor

    Jiazhang Lian

    Article

    Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

    Insert

    Cas9-VPR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLJC6-Flag CASTOR1

    Plasmid

    #84496

    Purpose

    Lentiviral expression of CASTOR1

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

    Insert

    CASTOR1 (GATSL3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    sestrin2

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only