Skip to main content
Addgene
Showing: 501 - 550 of 1292 results

  18. pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF)

    Plasmid
    #101139
    Purpose
    Luciferase reporter plasmid. Note that both renilla luciferase and ORF-firefly luciferase are encoded by a single mRNA and both are translated independently via internal ribosomal binding sites.
    Depositor
    William Kaelin
    Article
    Lu et al Science. 2014 Jan 17;343(6168):305-9. do
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    101139-plasmid-map-sequence-id-194900

    Enlarge

  30. 2xFKBP-mCh-KDEL

    Plasmid
    #173010
    Purpose
    FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of mCherry. Contains IRES following ORF for cloning cargo.
    Depositor
    Matthew Kennedy
    Article
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Insert
    2xFKBP-mCh-KDEL
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Chicken beta actin (CAG)
    Available since
    Aug. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    173010-plasmid-map-sequence-id-339203

    Enlarge

  32. pHX_HDR_hPAX7_IRES-EGFP-PGK-Neo

    Plasmid
    #160458
    Purpose
    Homology-directed recombination donor vector for EGFP reporter knock-in to human PAX7 3' UTR
    Depositor
    April Pyle
    Article
    Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi
    Inserts
    PAX7 Left/5' Homology Arm (PAX7 Human)
    PAX7 Right/3' Homology Arm (PAX7 Human)
    Use
    Insertion of a fluorescent reporter through homol…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    160458-plasmid-map-sequence-id-316796

    Enlarge

  36. DHFR-mNeon-GluA1

    Plasmid
    #173009
    Purpose
    For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Intracellular trafficking can be better visualized with mNeon.
    Depositor
    Matthew Kennedy
    Article
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Insert
    DHFR-mNeon-GluA1
    Use
    Tags
    DHFR and mNeon
    Expression
    Mammalian
    Mutation
    Promoter
    Chicken beta actin (CAG)
    Available since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    173009-plasmid-map-sequence-id-339113

    Enlarge

  44. TfR-moxGFP-DHFR

    Plasmid
    #173012
    Purpose
    For light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter. Fused to oxidation resistant version of GFP (moxGFP).
    Depositor
    Matthew Kennedy
    Article
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Insert
    TfR-moxGFP-DHFR
    Use
    Tags
    DHFR and moxGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    173012-plasmid-map-sequence-id-339204

    Enlarge

  46. pFSynW Substance P-pHluorin IRES mRuby3

    Plasmid
    #195699
    Purpose
    Lentiviral plasmid encoding first 68 residues of mouse preprotachykinin with a C-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoter
    Depositor
    Edwin Chapman
    Article
    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.
    Insert
    Tac1 (Tac1 Mouse)
    Use
    Lentiviral
    Tags
    pHluorin
    Expression
    Mutation
    Promoter
    Available since
    Oct. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    195699-plasmid-map-sequence-id-383681

    Enlarge

  50. DHFR-GFP-th-GluA1

    Plasmid
    #173008
    Purpose
    For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and GluA1.
    Depositor
    Matthew Kennedy
    Article
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Insert
    DHFR-GFP-th-GluA1
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    human synapsin
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    173008-plasmid-map-sequence-id-339527

    Enlarge

Showing: 501 - 550 of 1292 results