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173,387 results

Showing: 5501 - 5550 of 173387 results

  1. mCherry-Calnexin-N-14

    Plasmid
    #55005
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Calnexin (CANX Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    I474N in Calnexin
    Promoter
    CMV
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55005-plasmid-map-sequence-id-456544

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  3. pLyGo-Bs-1

    Plasmid
    #163137
    Purpose
    pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyL signal peptide and the LyGo casette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PamyL-PamyQ-PcryIIIA-cryIIIAstab49
    Available Since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    163137-plasmid-map-sequence-id-332146

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  4. pAAV_hSyn-DIO-PdCO-mScarlet-WPRE (AAV1)

    Viral Prep
    #198511-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV_hSyn-DIO-PdCO-mScarlet-WPRE (#198511). In addition to the viral particles, you will also receive purified pAAV_hSyn-DIO-PdCO-mScarlet-WPRE plasmid DNA. Syn-driven Cre-dependent expression of optimized PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Article
    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s
    Promoter
    Syn
    Tags
    mScarlet (Cre-dependent)
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only

  7. AAV-Syn-FLPX-rc [ChrimsonR-tdTomato] (AAV8)

    Viral Prep
    #128589-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from AAV-Syn-FLPX-rc [ChrimsonR-tdTomato] (#128589). In addition to the viral particles, you will also receive purified AAV-Syn-FLPX-rc [ChrimsonR-tdTomato] plasmid DNA. Syn-driven, Flp-dependent ChrimsonR-tdTomato expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Promoter
    Syn
    Tags
    tdTomato
    Available Since
    Feb. 14, 2022
    Availability
    Academic Institutions and Nonprofits only

  8. pSLIK DNM1L-V5 neo

    Plasmid
    #220363
    Purpose
    Lentiviral expression vector for an inducible V5-tagged DNM1L construct
    Depositor
    Kevin Janes
    Insert
    DNM1L (DNM1L Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220363-plasmid-map-sequence-id-436888

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  15. pQUASp-mCD8mCherry

    Plasmid
    #46164
    Purpose
    Expression of murine CD8mCherry in insects
    Depositor
    Christopher Potter
    Insert
    mCD8mCherry
    Expression
    Insect
    Available Since
    Aug. 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    46164-plasmid-map-sequence-id-65048

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  19. Human SAM library in lentiSAMv2 backbone

    Pooled Library
    #1000000078
    Purpose
    Genome-wide CRISPR gRNA pooled library for activation of human genes
    Depositor
    Feng Zhang
    Available Since
    June 27, 2016
    Availability
    Academic Institutions and Nonprofits only

  22. pAP1-3

    Plasmid
    #71258
    Purpose
    luciferase reporter with 3 copies of the stromelylisin gene AP-1 site in front of hGM-CSF -55 to +28 minimal promoter
    Depositor
    Peter Cockerill
    Insert
    3 copies of an AP-1 site from the human Stromelysin-1 gene (MMP3 Human)
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Promoter
    GM-CSF minimal
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    71258-plasmid-map-sequence-id-131888

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  27. ppyCAG_RNaseH1_WKKD

    Plasmid
    #111905
    Purpose
    Express WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybrids
    Depositor
    Xiang-Dong Fu
    Article
    Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.
    Insert
    RNASEH1 (RNASEH1 Human)
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Changed D 210 to N, W 43 to A, K 59 to A, K 60 to…
    Available Since
    July 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    111905-plasmid-map-sequence-id-212872

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  28. p46Cpf1-OP2

    Plasmid
    #98592
    Purpose
    Produces lambda Red and Cpf1 for recombination and selection. The plasmid is combined with a donor plasmid (with crRNA and template) for rapid genome editing in E.coli.
    Depositor
    Qiong Wu
    Article
    Ao et al Front Microbiol. 2018 Oct 9;9:2307. doi:
    Insert
    FnCpf1
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Codon optimized for E. coli
    Promoter
    araBAD
    Available Since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    98592-plasmid-map-sequence-id-201329

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  29. pAAV-mDlx-GFP-Fishell-1 (AAV8)

    Viral Prep
    #83900-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-mDlx-GFP-Fishell-1 (#83900). In addition to the viral particles, you will also receive purified pAAV-mDlx-GFP-Fishell-1 plasmid DNA. mDlx-driven expression of GFP in GABA-ergic interneurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Promoter
    mDlx
    Available Since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only

  31. p663-UBC-V5-miniTurbo-V5-STOP_IDG-K

    Plasmid
    #158158
    Purpose
    Gateway destination clone of miniTurbo-V5 (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    V5-miniTurbo-V5
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    158158-plasmid-map-sequence-id-314579

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  32. pCS2FLAG

    Plasmid
    #16331
    Depositor
    Peter Klein
    Insert
    Flag tag
    Use
    Vertebrate expression
    Tags
    Flag
    Mutation
    Flag tag inserted into pCS2+ polylinker
    Available Since
    Dec. 21, 2007
    Availability
    Academic Institutions and Nonprofits only
    16331-plasmid-map-sequence-id-316908

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  33. p663-UBC-TurboID-V5-eGFP_IDG-K

    Plasmid
    #135264
    Purpose
    Gateway destination clone of eGFP (as control) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    TurboID-V5-eGFP
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135264-plasmid-map-sequence-id-268064

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  34. pGBW-m4046757

    Plasmid
    #145657
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145657-plasmid-map-sequence-id-298331

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  36. pCAS

    Plasmid
    #60847
    Purpose
    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast
    Depositor
    Jamie Cate
    Article
    Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife
    Inserts
    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    NLS/His8 and TTT 3' extension prior to sgRNA
    Expression
    Bacterial and Yeast
    Mutation
    L4 is UUCG tetraloop and guide targets LYP1 (CATA…
    Available Since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    60847-plasmid-map-sequence-id-197511

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  40. AAV pEF1a-DIO-FLPo-WPRE-hGHpA (AAV5)

    Viral Prep
    #87306-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from AAV pEF1a-DIO-FLPo-WPRE-hGHpA (#87306). In addition to the viral particles, you will also receive purified AAV pEF1a-DIO-FLPo-WPRE-hGHpA plasmid DNA. EF1a-driven, Cre dependent expression of the site-specific recombinase FlpO. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Li Zhang
    Article
    Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.
    Promoter
    EF1a
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only

  43. pAAV-hDlx-GqDREADD-dTomato-Fishell-4 (AAV Retrograde)

    Viral Prep
    #83897-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hDlx-GqDREADD-dTomato-Fishell-4 (#83897). In addition to the viral particles, you will also receive purified pAAV-hDlx-GqDREADD-dTomato-Fishell-4 plasmid DNA. hDlx-driven expression of GqDREADD (hM3D) and nuclear localized dTomato (physically separate) for activation of interneurons. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Promoter
    hDlx
    Tags
    dTomato
    Available Since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only

  44. pmVen-TopBP1

    Plasmid
    #211413
    Purpose
    mammalian expression of human TopBP1 N-terminally fused to mVenus
    Depositor
    Helmut Pospiech
    Insert
    TopBP1 (TOPBP1 Human)
    Tags
    mVenus
    Expression
    Mammalian
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211413-plasmid-map-sequence-id-420517

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  49. pCMV-GATA3gfp

    Plasmid
    #83819
    Purpose
    Mammalian expression of GATA3 fused to GFP
    Depositor
    Douglas Engel
    Insert
    GATA3 (Gata3 Mouse)
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    83819-plasmid-map-sequence-id-166278

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Showing: 5501 - 5550 of 173387 results