164,169 results
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Plasmid#209381PurposeGateway compatible plasmid for TurboID-based proximity labelling experiment in plantaDepositorTypeEmpty backboneUseTagsExpressionPlantMutationTurboID gene sequence C249APromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only
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pAAV.Syn.NES.jRCaMP1a.WPRE.SV40
Plasmid#100848PurposeAAV expression of jRCaMP1a, a red fluorescent calcium sensor protein, from the Synapsin promoterDepositorHas ServiceAAV1 and AAV9InsertjRCaMP1a
UseAAVTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hGOLGA2-C-PEN2
Plasmid#183649PurposeLentiviral expression of a golgi-residential PEN2.DepositorUseLentiviralTagsHAExpressionMutationWTPromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TetON-Bmpr1A-ca
Plasmid#217536PurposeAAV vector to drive the expression of Cre- and doxycycline-dependent expression of HA-epitope tagged constitutively-active BMPR1A receptorDepositorArticleInsertconstitutively active BMP receptor 1a (BMPR1A Human)
UseAAVTagsHAExpressionMammalianMutationPromoterTet operon (TetO)Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAdEasy-EGFP-CLASP2 340-1362
Plasmid#24522DepositorInsertCLASP2 (340-1362) (CLASP2 Human)
UseAdenoviralTagsEGFPExpressionMammalianMutationCLASP2 deletion mutant that retains MT lattice bi…PromoterAvailable sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
TFORF1847
Plasmid#142868PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertETS1 (ETS1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-CD4-sTb(C)-M13-GFP
Plasmid#219784PurposesTb(C) half of CaST for neuron expressionDepositorInsertCD4-sTb(C)-M13-GFP
UseAAVTagsHAExpressionMammalianMutationPromoterSynapsinAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
LRRC3_pcDNA6.2/EmGFP-Bsd
Plasmid#176972PurposeMammalian expression vector encoding LRRC3 and EmGFP-BsdDepositorInsertLRRC3 (LRRC3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRXG
Plasmid#113643PurposeThis construct contains an amber stop codon within the linker between the two genes. The read through rate of the amber stop codon can be determined using the ratio of GFP signal to RFP signal.DepositorInsertmRFP1-linker-sfGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMTMVi-N
Plasmid#118756PurposeIntermediate plasmid to build tobacco mosaic virus recombinant clones that do not move systemically throughout the plant.DepositorInsertTobacco mosaic virus cDNA fragment with a cloning polylinker
UseIntermediate to build tobacco mosaic virus recomb…TagsExpressionMutationPromoterAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tet-off Full length Tau (0N4R, P301L/V337M)
Plasmid#188573PurposeDox-repressible expression of the 0N4R Tau isoformDepositorInsert0N4R Tau (MAPT Human)
UseLentiviralTagsExpressionMammalianMutationP301L and V337MPromoterpTight TREAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pylT-N346A/C348A
Plasmid#127411PurposeDouble mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethersDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
UseTagsExpressionBacterialMutationN346A-C348APromoteraraBADAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO human shRNA 2 DEPTOR
Plasmid#21336DepositorInsertDEPTOR shRNA 2 (DEPTOR Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
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SPOP-V5 (F133V)
Plasmid#169725PurposeMammalian Expression of mouse SPOP F133VDepositorInsertSPOP (Spop Mouse)
UseTagsV5ExpressionMammalianMutationF133VPromoterCMVAvailable sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pp2.1-PT5-lacI-gusA-specR-pp2.2-IMBB
Plasmid#30505DepositorInsertbeta-glucuronidase (gusA Escherichia coli)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHnCBS1D
Plasmid#52065Purposeexpresses nine genes from the H. neapolitanus carboxysome operon and CSOS1D; IPTG-inducibleDepositorInsertCbbL, CbbS, CSOS2, CSOS3, CSOS4A, CSOS4B, CSOS1C, CSOS1A, CSOS1B, CSOS1D
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
mLama1 AAV sgRNA 1, 2, 5
Plasmid#135339PurposeExpression plasmid of VP64-SadCas9 sgRNA 1, 2 and 3DepositorInsertLama1 VP64-dCas9 sgRNA Guides
UseAAVTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET His6 GST TEV LIC cloning vector (1G)
Plasmid#29655DepositorTypeEmpty backboneUseTags6xHis, GST, and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
TIRAP-GFP
Plasmid#52739PurposeRetroviral vector expresses WT hTIRAPfused to GFP. Also contains IRES-hCD2 as selectable markerDepositorInsertToll/IL-1 Receptor adaptor protein (TIRAP Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
Antibody#180098-rAbPurposeAnti-Kv1.1 K+ channel (Rat) recombinant mouse monoclonal antibodyDepositorRecommended ApplicationsImmunohistochemistry and Western BlotReactivityHuman, Mouse, Nematode, and RatSource SpeciesMouseIsotypeIgG2aTrial SizeAvailable to purchaseAvailable sinceMarch 30, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits
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pTNT-B18R
Plasmid#58978Purposeexpresses B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNsDepositorInsertB18R
UseTagsExpressionBacterialMutationPromoterT7/Sp6Available sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPEE7
Plasmid#128357PurposeRecycable marker for use in Cryptococcus neoformans. Contains the amdS1 counterselectable marker flanked by direct repeats.DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterACT1Available sinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
SPOP-V5 (W131G)
Plasmid#169724PurposeMammalian Expression of mouse SPOP W131GDepositorInsertSPOP (Spop Mouse)
UseTagsV5ExpressionMammalianMutationW131GPromoterCMVAvailable sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSGAb-km
Plasmid#121999PurposeA sgRNA expression plasmid for genome editing in Acinetobacter baumanniiDepositorInsertnone
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe-CD44v4
Plasmid#107969Purposeexpression of CD44 variantDepositorInsertmCD44v4 (Cd44 Mouse)
UseRetroviralTagsExpressionMammalianMutationsplice variant; see Depositor Comments belowPromoterAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJYScr
Plasmid#132719PurposePlasmid for an easy-to-construct crRNA delivery vector using hybridized oligonucleotides. Constitutive transcription of crRNA, Spectinomycin resistanceDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTALYM4SpMi-01
Plasmid#47879PurposeTALE-mRuby2 against Mus spretus minor satellite sequence containing two SNPsDepositorInsertTALE-mRuby2
UseTALENTagsmRuby2ExpressionMammalianMutationPromoterCAGAvailable sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
TFORF2918
Plasmid#142172PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNFIB (NFIB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
AFDN_PDZ_1
Plasmid#103938PurposeProtein expression and purification of TRITHORAX PDZ domainDepositorInsertAFDN (AFDN Human)
UseTagsGSTExpressionBacterialMutationcontains AA983-1110 of AFDNPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pInducer-TAZ-S89A
Plasmid#213588PurposeDoxycycline inducible expression of TAZ-S89A cDNA in mammalian cellsDepositorInsertTAZ (WWTR1 Human)
UseLentiviralTags2xHAExpressionMammalianMutationS89APromoterAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRYG
Plasmid#113642PurposePositive control: shows the ratio of GFP signal to RFP signal with a tyrosine residue in the linker that connects the two genes.DepositorInsertmRFP1-linker-sfGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q66
Plasmid#111735PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ66 (polyQ repeat)PromoterCMV and P10Available sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FLEX-EGFP-WPRE
Plasmid#51502PurposeCan be used to generate AAV virus that will express EGFP in the presence of CreDepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertEGFP
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
lvl0 eCFP CDS
Plasmid#153397PurposeCyan fluorescent protein coding sequenceDepositorInserteCFP CDS
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
GAL4-DrBphP
Plasmid#158986PurposeExpresses DrBphP fused with an N-terminal GAL4 DNA binding domain in mammalian cellsDepositorInsertGAL4-DrBphP
UseLentiviralTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQStrep2-CRYM
Plasmid#31293DepositorInsertcrystallin, mu (CRYM Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
FUGW-Cytosol-TurboID
Plasmid#200961Purpose3rd gen lentiviral plasmid for expressing cytosol localized TurboID with EGFPDepositorInsertCytosol-TurboID
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
SPOP-V5 (BTB+BACK)
Plasmid#169723PurposeMammalian Expression of mouse SPOP-BTB+BACK (L186D, L190D, L193D, I217K, Y353E)DepositorInsertSPOP (Spop Mouse)
UseTagsV5ExpressionMammalianMutationL186D, L190D, L193D, I217K, Y353EPromoterCMVAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-hIL2RN gRNA_multi1-3-MS2-Puro
Plasmid#192684PurposeLentiviral expression of multi gRNAs targeting hIL1RN promoter to activate human IL1RN transcriptionDepositorInsertHuman IL1RN activating gRNAs #1,2,3 (IL1RN Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBa.Halo-LDLR
Plasmid#162717PurposeMammalian expression of human LDLRDepositorInsertLDLR (LDLR Human)
UseTagsHaloExpressionMammalianMutationnonePromoterChicken Beta actinAvailable sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL2-p21 promoter-Luc
Plasmid#33021DepositorInsertp21 promoter (CDKN1A Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterp21Available sinceApril 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
PGKp-GFP-YAP (S94A)
Plasmid#174173PurposeLentiviral vector to constitutively express TEAD-binding mutant YAP under control of the human PGK promoter.DepositorInsertYAP (YAP1 Human)
UseLentiviralTags3x FLAG tagExpressionMammalianMutationSerine 94 mutated to AlaninePromoterPGKAvailable sinceAug. 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
mRFP-Rab5
Plasmid#14437PurposeMammalian expression of Rab5 fused to mRFP. Used for localization to the early endosome, and other early endosomal vacuoles.DepositorInsertRab 5 (RAB5A Human)
UseTagsmRFPExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGFP Cortactin
Plasmid#50728Purposemammalian expression of GFP-Cortactin. Note- This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.DepositorInsertCortactin (CTTN Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2FE-ABE8e-SpRY
Plasmid#213008PurposeA lentiviral vector expressing the ABE8e-SpRY base editor and an sgRNA cloning siteDepositorInsertABE8e-SpRY-D10A
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A nickase variant of SpRYPromoterEf-1aAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR_5xUAS-BFP-WPRE_PGK-HygR-bGHpolyA
Plasmid#216665PurposeExpresses Gal4 inducible BFP, with hygromycin resistanceDepositorInsertsBFP
HygromycinR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
YAP/TAZ-TRE-mStrawberry reporter
Plasmid#158682PurposeThis plasmid is a YAP/TAZ pathway reporter. It has a YAP/TAZ-responsive synthetic promoter driving the expression of mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P1-Vinculin tail
Plasmid#107154Purposebacterial expressionDepositorInsertVinculin (VCL Human)
UseTagsGSTExpressionBacterialMutationPromoterPtacAvailable sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUltra-5HTP
Plasmid#178042PurposeExpress modified ScTrpRS/tRNA for genetic incorporation of 5HTP to TAG codonDepositorInsertsModified ScTrpRS for 5HTP incoporation
suppressor tRNA for 5HTP incorporation
UseTagsExpressionBacterialMutationT107C, P254T, C255APromoterAvailable sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only