We narrowed to 22,982 results for: CRISPR

1. pRU47

   Plasmid

   #167669

   Purpose

   ENTRY vector for gRNA7 cloning under pAtU6 promoter

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pAtU6
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMiniT:SEP-GluN1 TKIT donor

   Plasmid

   #169427

   Purpose

   Donor DNA for TKIT knockin of SEP to NR1 in mouse

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   Super ecliptic pHluorin (SEP)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. NM1-5S-tRNA-SgH

   Plasmid

   #128178

   Purpose

   Expresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoter

   Depositor

   Huimin Zhao

   Article

   Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

   Insert

   gRNA cloning cassette
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   5S rRNA-tRNA(Arg)

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ141C

   Plasmid

   #69292

   Purpose

   Gateway entry vector; single gRNA under OsU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJAT11

   Plasmid

   #204290

   Purpose

   For inserting a phiC31 attP site. HDR integrant is marked with removable ie1-DsRed cassette, which is expressed in abdomen and mouthparts.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   ie1-DsRed
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   ie1

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBA562

   Plasmid

   #186237

   Purpose

   pTet-RfxCas13d crRNA entry vector

   Depositor

   Jennifer Doudna

   Article

   Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pX330A-1x3

   Plasmid

   #58767

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCR-Hyg

   Plasmid

   #158708

   Purpose

   For cloning and constitutive expression of crRNAs in mycobacterium

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

   Insert

   crRNA
   

   Use

   CRISPR and Synthetic Biology; Mycobacteria expres…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Hsp60

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. VKG1-gRNA-pX330

   Plasmid

   #108671

   Purpose

   Cleavage of VKG1 sequence by CRISPR/Cas9

   Depositor

   Shigeo Sugano

   Article

   Sawatsubashi et al Sci Rep. 2018 Jan 12;8(1):593. doi: 10.1

   Insert

   gRNA for VKG1 sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSB700 lenti gRNA mcherry

    Plasmid

    #167910

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLH-sgRNA1-2XboxB

    Plasmid

    #75391

    Purpose

    sgRNA1-2XboxB

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1-2XboxB
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330S-5

    Plasmid

    #58781

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYPQ146

    Plasmid

    #69298

    Purpose

    Golden Gate recipient and Gateway entry vector; assembly of 6 gRNAs

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. NAX_CAGGS_CAS9_VPR_Puro

    Plasmid

    #167895

    Purpose

    PiggyBac compatible plasmid expressing Cas9-VPR

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LbCas12a-gRNA_BbsI_CAM

    Plasmid

    #183222

    Purpose

    LbCas12a gRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    LbCas12a guide RNA containing BbsI sites in spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHFS:SEP-rGluA1 TKIT donor

    Plasmid

    #169438

    Purpose

    Donor DNA for TKIT knockin of SEP to GluA1 in rat

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    Super ecliptic pHluorin (SEP)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCas9

    Plasmid

    #162606

    Purpose

    Pre-express Cas9 in yeast

    Depositor

    Lars Steinmetz

    Article

    Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

    Insert

    Tef1-Cas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSB700 lenti gRNA EYFP

    Plasmid

    #167906

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX330S-6

    Plasmid

    #58782

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJAT75

    Plasmid

    #204306

    Purpose

    Flippase under heat shock control. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ie1

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSB700 lenti gRNA Blasto_zhang2.0

    Plasmid

    #167912

    Purpose

    Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pX459_gRNA-AAVS1_hspCas9

    Plasmid

    #193309

    Purpose

    Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

    Insert

    Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    not applicable

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMiniT:SEP-GluA1 TKIT donor

    Plasmid

    #169418

    Purpose

    Donor DNA for TKIT knockin of SEP to GluA1 in mouse

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    Super ecliptic pHluorin (SEP)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pYPQ141B

    Plasmid

    #69291

    Purpose

    Gateway entry vector; single gRNA under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. bRA90

    Plasmid

    #100951

    Purpose

    Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. LEU2 marker

    Depositor

    James Haber

    Article

    Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFETCh_ZBTB1

    Plasmid

    #128235

    Purpose

    Donor vector for 3' FLAG tag of human ZBTB1

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ZBTB1 Homology arms
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJAT62

    Plasmid

    #204301

    Purpose

    Express phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ie1

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMV261-Cas12a

    Plasmid

    #158707

    Purpose

    expresses codon-optimized FnCpf1

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    FnCpf1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetO

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pX330A-1x4

    Plasmid

    #58768

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRC319

    Plasmid

    #61272

    Purpose

    Also referred to as pΦRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, kanamycin resistance cassette and f1 origin for packaging in M13 particles.

    Depositor

    Timothy Lu

    Article

    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

    Insert

    Cas9, tracrRNA, crRNA
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pICSL1P1_crU626

    Plasmid

    #191776

    Purpose

    Cas12a guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSB700 lenti gRNA Puro_zhang2.0

    Plasmid

    #167919

    Purpose

    Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. p221z-CAS9p-t35s

    Plasmid

    #118385

    Purpose

    Entry clone containing CAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

    Insert

    CAS9p
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJAT43

    Plasmid

    #204299

    Purpose

    For introducing heat shock inducble B3 recombinase. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ie1

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pJAT56

    Plasmid

    #204300

    Purpose

    Express phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    3XP3-EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    3XP3

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pmax-3xFLAG_3xNLS_dPspCas13b(AAAA)_2xNLS-Clover

    Plasmid

    #196829

    Purpose

    Express dpdpCas13b with AAAA mutation and Clover

    Depositor

    Albert Cheng

    Article

    Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

    Insert

    dpspCas13b(AAAA)
    

    Use

    Tags

    3XFLAG and NES

    Expression

    Mammalian

    Mutation

    K367A, D369A and K370A

    Promoter

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBLO1808_arC9_2xNLS_human

    Plasmid

    #74491

    Purpose

    Human codon optimized plasmid to express arC9 t2a mCherry w/2xNLS and a sgRNA

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    arC9
    

    Use

    Tags

    t2a mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pADH110

    Plasmid

    #90982

    Purpose

    NAT-marked pSNR52 promoter fragment for use in gRNA expression cassette stitching PCR; for use with pADH119, 139, or 147 to generate target-specific gRNA expression cassette.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Inserts

    NAT 2 of 2
    pSNR52
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pYPQ145

    Plasmid

    #69297

    Purpose

    Golden Gate recipient and Gateway entry vector; assembly of 5 gRNAs

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBLO1806_Cas9_2xNLS_human

    Plasmid

    #74493

    Purpose

    Human codon optimized plasmid to express Cas9 t2a mCherry w/2xNLS and a sgRNA

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    Cas9
    

    Use

    Tags

    t2a mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge