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We narrowed to 11,387 results for: mpl

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Showing: 3361 - 3390 of 11387 results

  8. pJH104

    Plasmid
    #48262
    Purpose
    Template plasmid for PCR-based transplant of Saccharomyces cerevisiae TEF1 promoter in yeast.
    Depositor
    Ronald Davis
    Article
    Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10
    Inserts
    TEF1p 5' fragment
    URA3 5' fragment
    TEF1p 3' fragment
    Use
    Routine cloning vector
    Mutation
    From SK1 background. -343 to -1 relative to TEF1 …
    Promoter
    TEF1p
    Available Since
    Feb. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    48262-plasmid-map-sequence-id-69710

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  9. pBridge-tyrosinase.σ2

    Plasmid
    #197415
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-2 σ2
    Tags
    GAL4-DNA binding domain fragment, HA tag, and nuc…
    Expression
    Yeast
    Mutation
    encodes R42G substitution, contains silent substi…
    Promoter
    ADH1 and MET25
    Available Since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197415-plasmid-map-sequence-id-388893

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  10. pJS288

    Plasmid
    #200714
    Purpose
    To express yeast Hxt1 hexose transporter in yeast
    Depositor
    Jens Boch
    Article
    Fuhrmeister et al Bio Protoc. 2023 Aug 5;13(15):e4733. doi
    Inserts
    PMA1 promoter (PMA1 Budding Yeast)
    Hxt1 (HXT1 Budding Yeast)
    ADH2 Ter (ADH2 Budding Yeast)
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Yeast
    Mutation
    domesticated for MoClo cloning (removal of intern…
    Available Since
    Aug. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    200714-plasmid-map-sequence-id-404927

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  11. AAVS1-TetOn-3XFLAG-renilla-beta-globin-PTC39-AAVS1

    Plasmid
    #184398
    Purpose
    Donor plasmid for stable integration of Renilla luciferase NMD(+) PTC39 reporter at AAVS1
    Depositor
    Robert Bradley
    Article
    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:
    Insert
    Renilla luciferase beta-globin-PTC39
    Use
    CRISPR; Donor plasmid for hdr
    Tags
    3X FLAG
    Expression
    Mammalian
    Mutation
    PTC in beta-globin at amino acid position 39
    Promoter
    Tet-On 3G
    Available Since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    184398-plasmid-map-sequence-id-362369

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  12. p1.1-mCherry

    Plasmid
    #162772
    Purpose
    Fluorescent reporter for CHO expression studies
    Depositor
    Ivan Vorobiev
    Article
    Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2
    Insert
    red fluorescent protein
    Expression
    Mammalian
    Mutation
    consensus Kozak sequence (GCCGCCATGG) added befor…
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available Since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    162772-plasmid-map-sequence-id-339102

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  13. p1.2-GS-eGFP

    Plasmid
    #162771
    Purpose
    Fluorescent reporter for CHO expression studies
    Depositor
    Ivan Vorobiev
    Article
    Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2
    Insert
    enhanced green fluorescent protein
    Expression
    Mammalian
    Mutation
    consensus Kozak sequence (GCCGCCATGG) added befor…
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available Since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    162771-plasmid-map-sequence-id-339094

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  14. pU6-(BsaI)_CBh-UC-Cas9-T2A-mCherry

    Plasmid
    #135014
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to C-terminus of Cas9, linked to mCherry via a T2A peptide
    Depositor
    Yosef Shaul
    Article
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Insert
    humanized S. pyogenes Cas9
    Tags
    3x Flag, 3xFLAG (N terminal on insert), NLS, and …
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the C-termi…
    Promoter
    CBh
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    135014-plasmid-map-sequence-id-267599

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  16. pATP416-­pluxO5-­ptetO7-­crtYBI

    Plasmid
    #165975
    Purpose
    Expresses carotenoid biosynthesis gene in response to homoserine lactone and doxycycline in yeast expressing LuxTA and rtTA
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:
    Inserts
    Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
    Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
    BTS1 (BTS1 Budding Yeast)
    Use
    Synthetic Biology
    Expression
    Yeast
    Mutation
    C1281A, G1698A and C66A, C1359T
    Promoter
    Synthetic promoter (pluxO5), Synthetic promoter (…
    Available Since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    165975-plasmid-map-sequence-id-328841

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  17. pDONR P2R-P3-IRES_ECFP

    Plasmid
    #48354
    Purpose
    Contributes an IRES-ECFP cassette as the 3’-module during MultiSite Gateway cloning of a bi-cistronic mRNA shared with a two-part fusion protein encoded by the 5′- and middle modules.
    Depositor
    Anna Planas, Tomas Santalucia
    Article
    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:
    Insert
    IRES_ECFP
    Use
    Gateway Cloning
    Mutation
    ECFP translation is mediated by an IRES. Contains…
    Promoter
    none
    Available Since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    48354-plasmid-map-sequence-id-70631

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  18. YFP(159-238)-gamma-7 in pcDNAI/Amp

    Plasmid
    #54473
    Purpose
    A carboxyl-terminal YFP fragment was fused to Ggamma-7. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8
    Insert
    YFP(159-238)-gamma-7 (GNG7 Human, Aequorea victoria)
    Tags
    YFP(159-238) was fused to the amino terminus of G…
    Expression
    Mammalian
    Mutation
    Ggamma-7 was amplified by PCR, which added a BamH…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    54473-plasmid-map-sequence-id-115045

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  19. CFP(159-238)-gamma-2 in pcDNAI/Amp

    Plasmid
    #55777
    Purpose
    A C-terminal CFP fragment was fused to Ggamma-2. When co-expressed with an N-terminal mCerulean, CFP, or YFP fragment fused to a Gbeta subunit with which it interacts a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    CFP(159-238)-gamma-2 (GNG2 Human, Aequorea victoria)
    Tags
    CFP(159-238) was fused to the amino terminus of G…
    Expression
    Mammalian
    Mutation
    CFP(159-238) includes a substitution of His for A…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55777-plasmid-map-sequence-id-115196

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Showing: 3361 - 3390 of 11387 results