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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,383 results for: ENA

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Showing: 5751 - 5800 of 12383 results

  6. pMOD_B2121

    Plasmid
    #91065
    Purpose
    Module B, Promoter: GmUbi, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    Use
    CRISPR
    Promoter
    GmUbi
    Available Since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91065-plasmid-map-sequence-id-181862

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  8. pMOD_C2516

    Plasmid
    #91084
    Purpose
    Module C, Promoter: At7SL, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    Use
    CRISPR
    Promoter
    At7SL
    Available Since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91084-plasmid-map-sequence-id-182048

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  11. pDIRECT_22D

    Plasmid
    #91136
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, H840A
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    91136-plasmid-map-sequence-id-182475

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  22. pDIRECT_21D

    Plasmid
    #91131
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:hpt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, H840A
    Available Since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91131-plasmid-map-sequence-id-182255

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  31. pMOD_C2516b

    Plasmid
    #91085
    Purpose
    Module C, Promoter: At7SL, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, structurally optimized gRNA scaffold, (+ BaeI site for GT donor cloning), Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning
    Use
    CRISPR
    Promoter
    At7SL
    Available Since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91085-plasmid-map-sequence-id-182050

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  35. pT7-agrBD-IV

    Plasmid
    #53440
    Purpose
    Derivative of pT7-agrBD-I with a point mutation in the agrD gene to change AIP coding region from type-I to type-IV
    Depositor
    Cynthia Collins
    Article
    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003
    Insert
    agrBD locus (with D29Y mutation in agrD)
    Use
    Synthetic Biology
    Tags
    V5 epitope, 6x HIS (not expressed on agrB or agrD…
    Expression
    Bacterial
    Mutation
    D to Y mutation of residue 29 of the agrD gene (c…
    Promoter
    T7-lac
    Available Since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    53440-plasmid-map-sequence-id-83235

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  43. pXW117As(RS-E11)

    Plasmid
    #123145
    Purpose
    Arsenic sensor with two-layered amplifier cascade. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-30gfp-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-30gfp-t
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    J117, ParsR, PhrpLE, Pecf11
    Available Since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123145-plasmid-map-sequence-id-243995

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  48. pChlr-1SE

    Plasmid
    #52140
    Purpose
    Encodes module 1 with amino acids 12S-16E for recognition of nt 8G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 25-60 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 16Q to 16E in Module 1
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  49. pChlr-2SE

    Plasmid
    #52144
    Purpose
    Encodes module 2 with amino acids 12S-16E for recognition of nt 7G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 61-96 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16E in Module 2
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  50. pChlr-3SE

    Plasmid
    #52148
    Purpose
    Encodes module 3 with amino acids 12S-16E for recognition of nt 6G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 97-132 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12C to 12S and 16Q to 16E in Module 3
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 5751 - 5800 of 12383 results