We narrowed to 29,245 results for: pET

1. pET28a-mH6-Cas12g1

   Plasmid

   #120879

   Purpose

   Expresses E. coli codon optimized Cas12g1 effector in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Cas12g1
   

   Use

   CRISPR

   Tags

   mH6

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pet42c(+)-GST-USP13

   Plasmid

   #61749

   Purpose

   express N-terminally GST tagged human USP13

   Depositor

   Yihong Ye

   Article

   Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

   Insert

   USP13 (USP13 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pET-15b-PTS

   Plasmid

   #108953

   Purpose

   heterologous expression of PTS of P. cablin in E. coli

   Depositor

   Frank Schulz

   Article

   Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

   Insert

   Patchoulol synthase (PTS)
   

   Tags

   His6-Tag

   Expression

   Bacterial

   Available Since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-NLS-M-MLV-RT

   Plasmid

   #181804

   Purpose

   pET28a-NLS-M-MLV RT

   Depositor

   Erik Sontheimer

   Article

   Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

   Insert

   NLS-M-MLV-RT
   

   Expression

   Bacterial

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET15b His6-ecDHFR (WT)

   Plasmid

   #73188

   Purpose

   bacterial express ecDHFR protein for washing out TMP ligand for pBMN DHFR(DD)-YFP (Plasmid #29325) and pBMN YFP-DHFR(DD) (Plasmid #29326)

   Depositor

   Thomas Wandless

   Insert

   folA dihydrofolate reductase (folA E. coli)
   

   Tags

   His 6X

   Expression

   Bacterial

   Mutation

   none

   Available Since

   Feb. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

6. wt HIV-1 integrase in pET15b

   Plasmid

   #61668

   Purpose

   wild type HIV-1 integrase in pET-15b

   Depositor

   Robert Craigie

   Article

   Jenkins et al J Biol Chem. 1996 Mar 29;271(13):7712-8.

   Insert

   wild type HIV-1 integrase
   

   Tags

   His

   Promoter

   T7 promotor

   Available Since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pET-6xHis-GFP(+12)

   Plasmid

   #199161

   Purpose

   used to express a supercharged GFP variant in E. coli

   Depositor

   Allie Obermeyer

   Article

   Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

   Insert

   GFP(+12)
   

   Tags

   MGHHHHHHGGA

   Mutation

   E16R, E44K, D86K, D112K, T115K, D127R, I138R, E15…

   Promoter

   T7

   Available Since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21b Apaf1 1-591

   Plasmid

   #26642

   Depositor

   Guy Salvesen

   Article

   Pop et al Mol Cell. 2006 Apr 21. 22(2):269-75.

   Insert

   Apoptotic protease activating factor 1 (APAF1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Amino Acids 1-591

   Available Since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pET-Cre-6xHis

   Plasmid

   #62939

   Purpose

   Expression of Cre-6xHis in bacterial of cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   Cre recombinase
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETSXM2-pLacI-GFP

    Plasmid

    #174617

    Purpose

    Bacterial expression of GFP.

    Depositor

    I-Son Ng

    Article

    I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pLacI

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-Hook3 aa 1-552

    Plasmid

    #74614

    Purpose

    Expresses a truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-552 (HOOK3 Human)
    

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET15-His-∆NGlpG

    Plasmid

    #155131

    Purpose

    Expresses N-terminally His-tagged E. coli GlpG (residues 87-276) from pET15b

    Depositor

    Sinisa Urban

    Article

    Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

    Insert

    GlpG (glpG Escherichia coli)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    insert codes for GlpG residues 87-276

    Promoter

    T7

    Available Since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-6xHis-GFP(+18)

    Plasmid

    #199162

    Purpose

    used to express a supercharged GFP variant in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(+18)
    

    Tags

    MGHHHHHHGGA

    Mutation

    E16R, D29K, E42K, E44K, D86K, D112K, T115K, D127R…

    Promoter

    T7

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-HIV p24

    Plasmid

    #212195

    Purpose

    Bacterial expression plasmid for production of HIV p24 protein with a polyhistidine tag at the N-terminus

    Depositor

    Richard C. Willson

    Insert

    Chain A, Capsid protein p24, HIV type 1
    

    Tags

    Polyhistidine

    Expression

    Bacterial

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET_RGG-RGG-RGG

    Plasmid

    #124938

    Purpose

    Expression of RGG-RGG-RGG

    Depositor

    Matthew Good, Daniel Hammer, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-DUET-1-mRhubarb713-HO

    Plasmid

    #141199

    Purpose

    Expresses mRhubarb713 fluorescent protein and heme oxygenase

    Depositor

    Elad Firnberg

    Article

    Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

    Inserts

    mRhubarb713
    heme oxygenase
    

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    R134H, W309R, Q310A compared to iRFP

    Promoter

    T7

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-6xHis-GFP(0)

    Plasmid

    #199166

    Purpose

    used to express a neutral GFP variant in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(0)
    

    Tags

    MGHHHHHHGGA

    Mutation

    E16R, I138R, D207R, N222K, L246R

    Promoter

    T7

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-Gate2 mazEF

    Plasmid

    #117117

    Purpose

    Destination vector with mazEF toxin antitoxin system

    Depositor

    Stephan Gruber

    Article

    Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

    Type

    Empty backbone

    Use

    Backbone for golden gate assembly for gene target…

    Available Since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21b-Tipin-His

    Plasmid

    #23122

    Depositor

    Aziz Sancar

    Article

    Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

    Insert

    Tipin (TIPIN Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pET21-pelB-LaM-3

    Plasmid

    #172769

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-3
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET-HIS-Sangamo

    Plasmid

    #40786

    Purpose

    for e. coli expression, Golden Gate Compatible TALEN incorporated with N Terminal 6xHIS Tag, Codon Optimized FokI

    Depositor

    Gang Bao

    Insert

    Transcription Activator Like Effector Nuclease
    

    Use

    TALEN

    Tags

    N Terminal 6xHIS

    Expression

    Bacterial

    Mutation

    +63 C Terminus, Codon Optimized FokI Endonuclease

    Promoter

    T7

    Available Since

    Dec. 12, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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22. pET28-Dnmt3a3L-sc27

    Plasmid

    #71827

    Purpose

    For bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.

    Depositor

    Albert Jeltsch

    Article

    Siddique et al J Mol Biol. 2013 Feb 8;425(3):479-91. do

    Insert

    Dnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Available Since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. pET-APOBEC1-YTHmut

    Plasmid

    #200159

    Purpose

    For bacterial expression of the APOBEC1-YTHmut fusion protein for protein purification

    Depositor

    Kate Meyer

    Article

    Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

    Insert

    APOBEC1-YTHmut
    

    Tags

    6xHis, HA, and MBP

    Expression

    Bacterial

    Mutation

    Codon optimized for E coli expression

    Promoter

    LacZ

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. BioBrick pET-24a(+)

    Plasmid

    #117802

    Purpose

    BioBrick pET vector for protein overexpression in E. coli

    Depositor

    Anthony Forster

    Article

    Du et al Biotechnol Bioeng. 2009 Dec 15;104(6):11

    Type

    Empty backbone

    Use

    Escherichia coli

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. pETM-30-GST-PfAgo_F

    Plasmid

    #146265

    Purpose

    Bacterial Expression of PfAgo

    Depositor

    Elisa Izaurralde

    Insert

    PfAgo
    

    Expression

    Bacterial

    Mutation

    one silent T1704C and one non silent mutation A90…

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28a-RBP-LOV2

    Plasmid

    #185792

    Purpose

    Encodes Light-oxygen-voltage-sensing domain 2 linked to RNA binding peptide (on N terminal end) via GS-linker

    Depositor

    Lizhong He

    Article

    Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864

    Insert

    RBP-LOV2
    

    Tags

    10xHis, TEV protease cleavage site after His Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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27. pET-Sac-Aβ(M1-42/C18C33)

    Plasmid

    #173759

    Purpose

    Expresses the AβC18C33 peptide.

    Depositor

    James Nowick

    Article

    Zhang et al Biochemistry. 2022 Feb 15;61(4):252-264.

    Insert

    Aβ(M1-42/C18C33) (APP Human)
    

    Expression

    Bacterial

    Mutation

    changed valine 18 to cysteine; changed glycine 33…

    Promoter

    T7

    Available Since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28a-pro-sumo1

    Plasmid

    #25101

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    pro-SUMO1 (SUMO1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Changed Serine 2 to Alanine

    Available Since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

29. pET sfGFP(150TAG)

    Plasmid

    #133455

    Purpose

    expresses sfGFP with an unnatural amino acid at the 150th position in bacterial cells

    Depositor

    Yu-Hsuan Tsai

    Article

    Williams et al Amino Acids. 2021 Jan;53(1):89-96. doi:

    Insert

    sfGFP
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAR127_StrepI_m(KS_MAT)_mouseL_ACP_H8_pET22b

    Plasmid

    #122850

    Purpose

    Expresses a designed construct of the condensing part fused to the ACP domain of murine type I fatty acid synthase (FASN) in Escherichia coli

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    m(KS_MAT)_mouseL_ACP (Fasn Mouse)
    

    Tags

    His8 tag and Twin-Strep tag

    Expression

    Bacterial

    Mutation

    wildtype

    Promoter

    T7 promoter

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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