Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

175,277 results

Showing: 221 - 240 of 175277 results

  7. pCBh_NLS_hfCas13X(Cas13X_M17YY)_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #190033
    Purpose
    vector for encoding a human codon-optimized High-fidelity Cas13X (hfCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP and mCherry driven by SV40 promoters
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Insert
    humanized hfCas13X
    Expression
    Mammalian
    Mutation
    Y672A,Y676A
    Promoter
    CBh, SV40, hU6
    Available Since
    Sept. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    190033-plasmid-map-sequence-id-488812

    Enlarge

  8. pCAG-FLAG-puro-PTPIP51(1-150_175-470)

    Plasmid
    #170537
    Purpose
    Expresses PTPIP51 FFAT-like motif (151-175) deletion mutant (1-150_176-470) in human HeLa cells after transfection
    Depositor
    Byung Il Lee
    Article
    Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:
    Insert
    Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
    Tags
    FLAG tag
    Expression
    Mammalian
    Mutation
    deleted amino acids 151-175
    Promoter
    CAG promoter
    Available Since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only

  9. mCherry-Rab7a-7

    Plasmid
    #55127
    Purpose
    Localization: Ras GTPase, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Rab7a (RAB7A Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    N117S in NM_004637.5
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55127-plasmid-map-sequence-id-311825

    Enlarge

  11. pSpot-Tag-GFP-GPI

    Plasmid
    #117061
    Purpose
    For mammalian expression of GPI-anchored GFP with N-terminal Spot-Tag
    Depositor
    Felix Hartlepp
    Insert
    GPI-anchored GFP
    Tags
    GPI-anchor and Spot-Tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    117061-plasmid-map-sequence-id-224390

    Enlarge

  13. pENN.AAV.CamKII 0.4.Cre.SV40 (AAV Retrograde)

    Viral Prep
    #105558-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pENN.AAV.CamKII 0.4.Cre.SV40 (#105558). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII 0.4.Cre.SV40 plasmid DNA. CamKII-driven Cre expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CaMKII
    Available Since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only

  14. pET-MCN-10His-mStayGold (E138D)

    Plasmid
    #211361
    Purpose
    Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.
    Depositor
    Mohan Balasubramanian
    Article
    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103
    Insert
    mStayGold
    Tags
    10xHis-tag
    Expression
    Bacterial
    Mutation
    E138D
    Promoter
    T7
    Available Since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    211361-plasmid-map-sequence-id-417678

    Enlarge

  18. pCMV-lyso-pHoenix

    Plasmid
    #70112
    Purpose
    Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes
    Depositor
    Christian Rosenmund
    Article
    Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n
    Insert
    lyso-pHoenix (Cd63 Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Rat, Mouse)
    Tags
    Arch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    70112-plasmid-map-sequence-id-418643

    Enlarge

Showing: 221 - 240 of 175277 results