168,037 results

1. Human CRISPRi sgRNA library Dolcetto in backbone XPR_050

   Pooled Library

   #1000000114

   Purpose

   Genome-wide CRISPR gRNA pooled library for inhibition of human genes

   Depositor

   John Doench, David Root

   Available Since

   July 13, 2018

   Availability

   Academic Institutions and Nonprofits only

2. pvimentin-emiRFP703

   Plasmid

   #136566

   Purpose

   Vimentin labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   vimentin-emiRFP703
   

   Tags

   emiRFP703

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-SEP-Glur2 (MCA10)

   Plasmid

   #64941

   Purpose

   mammalian expression of rat GluR2

   Depositor

   Jeremy Henley

   Article

   Ashby et al J Neurosci. 2004 Jun 2;24(22):5172-6.

   Insert

   GluR2 (Gria2 Rat)
   

   Tags

   SEP (superecliptic pHluorin)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. wtBxb1

   Plasmid

   #222337

   Purpose

   Plasmid for expressing wild type Bxb1 in mammalian cells

   Depositor

   David Liu

   Article

   Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

   Insert

   Codon-optimized wild type Bxb1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9_T2A_mCherry sg1-sg2

   Plasmid

   #192479

   Purpose

   All-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Tags

   T2A mCherry

   Expression

   Mammalian

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMRX-IB-pSu9-HaloTag7-mGFP

   Plasmid

   #184905

   Purpose

   Stable expression of pSu9-HaloTag7-mGFP in mammalian cells to measure mitophagy activity

   Depositor

   Noboru Mizushima

   Article

   Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

   Insert

   Presequence of Fo-ATPase subunit 9
   

   Tags

   HaloTag7 and monomeric EGFP

   Expression

   Mammalian

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-Con/Fon-GCaMP6F (AAV5)

   Viral Prep

   #137122-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV-Ef1a-Con/Fon-GCaMP6F (#137122). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Fon-GCaMP6F plasmid DNA. EF1a-driven, Cre and Flp-dependent expression of GCaMP6f. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   EF1a

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-endo-Nb37

   Plasmid

   #205514

   Purpose

   Endosome-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.

   Depositor

   Jin Zhang

   Article

   Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

   Insert

   endo-Nb37
   

   Tags

   2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-ATF6

   Plasmid

   #32955

   Purpose

   For visualization of GFP-ATF6 in mammalian cells.

   Depositor

   Ron Prywes

   Article

   Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

   Insert

   ATF6 (ATF6 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   M67L and M313I

   Promoter

   CMV

   Available Since

   Nov. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmGFP-P2A-K0-P2A-RFP

    Plasmid

    #105686

    Purpose

    Double fluorescent stalling reporter K0

    Depositor

    Ramanujan Hegde

    Article

    Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

    Inserts

    P2A-3xFLAG-VHPbeta
    K0
    P2A-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV (from vector)

    Available Since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEJS1099: Dual-sgRNA.Design 4

    Plasmid

    #159537

    Purpose

    Delivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vector

    Depositor

    Erik Sontheimer

    Article

    Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

    Insert

    Nme2Cas9 with two guide RNA cassettes
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    U1a

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 (AAV9)

    Viral Prep

    #100835-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 (#100835). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Promoter

    CAG

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

13. pGL3-U6-sgRNA-PGK-puromycin

    Plasmid

    #51133

    Purpose

    expresses sgRNA from the U6 promoter.

    Depositor

    Xingxu Huang

    Article

    Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENN.AAV.hSyn.Cre.WPRE.hGH

    Plasmid

    #105553

    Purpose

    AAV expression of Cre from hSyn promoter

    Depositor

    James M. Wilson

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. lenti-SpCas9 hygro

    Plasmid

    #104995

    Purpose

    This lentiviral construct delivers hSpCas9 and hygromycin resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. TrkB mEGFP

    Plasmid

    #83952

    Purpose

    FRET donor for dynamically measuring TrkB activity in neurons

    Depositor

    Ryohei Yasuda

    Article

    Harward et al Nature. 2016 Sep 28;538(7623):99-103. do

    Insert

    Tropomyosin related kinase B (Ntrk2 Mouse)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. BFP-KDEL

    Plasmid

    #49150

    Purpose

    visualization of the ER

    Depositor

    Gia Voeltz

    Article

    Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

    Insert

    BiP ER signal sequence (HSPA5 Human)
    

    Tags

    BFP and KDEL

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-S5E2-ChR2-mCherry (AAV9)

    Viral Prep

    #135634-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-S5E2-ChR2-mCherry (#135634). In addition to the viral particles, you will also receive purified pAAV-S5E2-ChR2-mCherry plasmid DNA. Expression of ChR2-mCherry under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Jordane Dimidschstein

    Article

    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

    Tags

    mCherry

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

19. pCXB-EBNA1

    Plasmid

    #41857

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    EBNA-1 (EBNA-1 human herpesvirus 4)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. TERT - WT

    Plasmid

    #213827

    Purpose

    Expresses human telomerase reverse transcriptase, the catalytic subunit of telomerase

    Depositor

    Coleman Lindsley

    Article

    Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

    Insert

    human TERT (TERT Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Tet-responsive promoter PTight, consisting of sev…

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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