161,398 results

1. pAAV-GFAP-hM3D(Gq)-mCherry (AAV5)

   Viral Prep

   #50478-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV-GFAP-hM3D(Gq)-mCherry (#50478). In addition to the viral particles, you will also receive purified pAAV-GFAP-hM3D(Gq)-mCherry plasmid DNA. GFAP-driven hM4D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Promoter

   GFAP

   Tags

   mCherry

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

2. p21-Reporter

   Plasmid

   #52432

   Purpose

   DNA damage inducible Red Fluorescent Protein for mammalian expression

   Depositor

   Joel Voldman

   Article

   Fendyur et al Anal Chem. 2014 Aug 5;86(15):7598-605. d

   Insert

   p21-p53 Tandem Response Element Promoter
   

   Use

   Tags

   TurboRFP

   Expression

   Mammalian

   Mutation

   Promoter

   13X repeated p21-p53 response element

   Available since

   Oct. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.TBG.PI.eGFP.WPRE.bGH (AAV8)

   Viral Prep

   #105535-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV.TBG.PI.eGFP.WPRE.bGH (#105535). In addition to the viral particles, you will also receive purified pAAV.TBG.PI.eGFP.WPRE.bGH plasmid DNA. TBF-driven eGFP expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   TBG

   Tags

   EGFP

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

4. pAAV-FLEX-tdTomato (AAV9)

   Viral Prep

   #28306-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. CAG-driven, Cre-dependent tdTomato expression control. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Edward Boyden

   Promoter

   CAG

   Tags

   tdTomato (Cre-dependent)

   Available since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

5. pCAG-YFP

   Plasmid

   #11180

   Purpose

   Mammalian expression vector for expression of YFP

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

   Insert

   EYFP from Aequorea victoria
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.CMV.Luc.IRES.EGFP.SV40

   Plasmid

   #105533

   Purpose

   AAV expression of Luciferase and EGFP from CMV promoter

   Depositor

   James M. Wilson

   Insert

   Luciferase
   

   Use

   AAV and Luciferase

   Tags

   IRES-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENN.AAV.CamKII 0.4.Cre.SV40 (AAV1)

   Viral Prep

   #105558-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pENN.AAV.CamKII 0.4.Cre.SV40 (#105558). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII 0.4.Cre.SV40 plasmid DNA. CamKII-driven Cre expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   CaMKII

   Tags

   None

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

8. pEGFP-LC3 (human)

   Plasmid

   #24920

   Purpose

   Mammalian expression of LC3 fused to EGFP

   Depositor

   Toren Finkel

   Article

   Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

   Insert

   LC3 (MAP1LC3B Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST (AAV9)

   Viral Prep

   #105448-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST (#105448). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST plasmid DNA. hSyn-driven expression of ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Hillel Adesnik

   Article

   Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:

   Promoter

   Syn

   Tags

   mRuby2

   Available since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

10. pT7-PEmax for IVT

    Plasmid

    #178113

    Purpose

    Template for in vitro transcription of PEmax

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax
    

    Use

    Template for in vitro transcription

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mutation

    Detailed in manuscript

    Promoter

    T7 (inactivated)

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Tet-pLKO-puro

    Plasmid

    #21915

    Purpose

    3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.

    Depositor

    Dmitri Wiederschain

    Article

    Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC57-LbNOX

    Plasmid

    #75285

    Purpose

    This plasmid contains human codon optimized sequence of LbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.

    Depositor

    Vamsi Mootha

    Article

    Titov et al Science. 2016 Apr 8;352(6282):231-5. doi

    Insert

    LbNOX
    

    Use

    Tags

    C-terminal FLAG tag with linker

    Expression

    Mutation

    Human codon optimized

    Promoter

    Available since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV pCAG-FLEX-EGFP-WPRE (AAV9)

    Viral Prep

    #51502-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from AAV pCAG-FLEX-EGFP-WPRE (#51502). In addition to the viral particles, you will also receive purified AAV pCAG-FLEX-EGFP-WPRE plasmid DNA. CAG-driven, Cre dependent EGFP expression control. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Promoter

    CAG

    Tags

    EGFP (Cre-dependent)

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

14. pGP-AAV-syn-jGCaMP7s-WPRE (AAV PHP.eB)

    Viral Prep

    #104487-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from pGP-AAV-syn-jGCaMP7s-WPRE (#104487). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7s-WPRE plasmid DNA. Synapsin-driven GCaMP7s calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Promoter

    Syn

    Tags

    None

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

15. c-Flag pcDNA3

    Plasmid

    #20011

    Purpose

    This pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.

    Depositor

    Stephen Smale

    Article

    Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCE-hSK

    Plasmid

    #41814

    Purpose

    Non-integrating (episomal) expression of human SOX2 and KLF4

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    SOX2, KLF4 (SOX2, KLF4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-TREtight-Ngn2:2A:Ascl1-PGK-Puro (XTP-N2A)

    Plasmid

    #84777

    Purpose

    Second generation lenti expressing Mash1 and Neurogenin for iN transdifferentation to neurons

    Depositor

    Fred Gage

    Article

    Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

    Insert

    Ngn2 and Mash1 with a T2A (NEUROG2, ASCL1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    pTIGHT

    Available since

    Nov. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CAG-GFP (AAV Retrograde)

    Viral Prep

    #37825-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. GFP expression control. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Promoter

    CAG

    Tags

    GFP

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

19. pCDH-CMV-mCherry-T2A-Puro

    Plasmid

    #72264

    Purpose

    Express mCherry and puromycin resistance gene under CMV promoter. 3rd gen lentiviral vector.

    Depositor

    Kazuhiro Oka

    Insert

    mCherry-T2A-Puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Ef1a-mCherry-IRES-Cre (AAV8)

    Viral Prep

    #55632-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-Ef1a-mCherry-IRES-Cre (#55632). In addition to the viral particles, you will also receive purified pAAV-Ef1a-mCherry-IRES-Cre plasmid DNA. Cre and bicistronic (IRES) mCherry expression under the EF1a promoter. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Promoter

    EF1a and Ef1a/IRES

    Tags

    mCherry

    Available since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only