We narrowed to 2,016 results for: abat

Showing: 551 - 600 of 2016 results

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pRK5-FLAG-p14

Plasmid

#42330

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

p14 (LAMTOR2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV, SP6

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FLAG-MTOR-V2006I

Plasmid

#69012

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

V2006I

Promoter

CMV

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
N1-p18-EGFP

Plasmid

#42334

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

p18 (LAMTOR1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-FLAG-Sestrin1 isoform1

Plasmid

#72594

Purpose

overexpression, codon optimized for mammalian expression

Depositor

David Sabatini

Article

Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

Insert

SESN1 (SESN1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized for mammalian expression, see dep…

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pLJM1-FLAG-metap2

Plasmid

#100510

Purpose

Expresses FLAG tagged metap2 in mammalian cells to make lentivirus

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

METAP2 (METAP2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
MelanoTag-Control-PURO (pLJC5-GPR143-mScarlet-1xMyc)

Plasmid

#160368

Purpose

Stable expression of GPR143-mScarlet-1xMyc fusion protein for use as a control with MelanoTag-PURO

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

GPR143 (GPR143 Human)

Use

Lentiviral

Tags

mScarlet-myc

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
HA Seh1L pRK5

Plasmid

#46331

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Seh1 (SEH1L Human)

Tags

HA

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
PMXS-SLC1A3

Plasmid

#72873

Purpose

The retroviral SLC1A3 vector was generated by cloning an human SLC1A3 PCR fragment (https://www.addgene.org/32813/)into the pMXS-ires-blast vector by Gibson Assembly

Depositor

David Sabatini

Article

Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

Insert

SLC1A3 (SLC1A3 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pRK5-FLAG-ATP6V0D1

Plasmid

#87931

Purpose

FLAG-tagged overexpression

Depositor

David Sabatini

Article

Zoncu et al Science. 2011 Nov 4;334(6056):678-83.

Insert

ATP6Vod1 (ATP6V0D1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-FLAG-Sestrin2

Plasmid

#72595

Purpose

overexpression

Depositor

David Sabatini

Article

Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

Insert

SESN2 (SESN2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized for mammalian expression, see dep…

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pRK5-FLAG-KPTN

Plasmid

#87041

Purpose

mammalian expression of KPTN

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

KPTN (KPTN Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-FLAG-ITFG2

Plasmid

#87044

Purpose

mammalian expression of ITFG2

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

ITFG2 (ITFG2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CMV-FLEX-TVAmCherry-2A-oG

Plasmid

#102985

Purpose

AAV expressing TVA-mCherry fusion and optimised Rabies Glycoprotein (oG) after CRE-mediated recombination

Depositor

Marco Tripodi

Article

Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

Inserts

TVA-mCherry fusion protein after CRE-mediated recombination
Optimised Rabies Glycoprotein (oG) after CRE-mediated recombination

Use

AAV; Adeno associated viral vector

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-HA-FNIP2

Plasmid

#72295

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FNIP2 (FNIP2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pRK5-HA-Sestrin2

Plasmid

#72593

Purpose

overexpression, codon optimized for mammalian expression

Depositor

David Sabatini

Article

Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

Insert

SESN2 (SESN2 Human)

Tags

HA

Expression

Mammalian

Mutation

codon optimized for mammalian expression, see dep…

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pDarmo.CMVT_v1

Plasmid

#133072

Purpose

Entry vector for cloning genes under an enhanced CMVT promoter for expression in mammalian cells. Version 1.0.

Depositor

Kacper Rogala, David Sabatini

Article

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Entry vector

Tags

3C-Flag-TwinStrep and TwinStrep-Flag-3C

Expression

Mammalian

Promoter

CMVT (Enhanced CMV)

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-FLAG-HBXIP

Plasmid

#42326

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

HBXIP (LAMTOR5 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV, SP6

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-ATP6V1D-HAGST

Plasmid

#87932

Purpose

C-term HA-GST tagged overexpression

Depositor

David Sabatini

Article

Zoncu et al Science. 2011 Nov 4;334(6056):678-83.

Insert

ATP6V1D (ATP6V1D Human)

Tags

HA-GST

Expression

Mammalian

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
3XFLAG_ALDOA in pCW57_tTA_Blast

Plasmid

#154210

Purpose

Dox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of ALDOA

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

ALDOA (ALDOA Human)

Use

Lentiviral

Tags

3X FLAG

Expression

Mammalian

Promoter

TRE repeats (dox off)

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-p18G2A-HA

Plasmid

#42327

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

p18 G2A (LAMTOR1 Human)

Tags

HA

Expression

Mammalian

Mutation

G2A

Promoter

CMV, SP6

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-FLAG-FNIP1

Plasmid

#72292

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FNIP1 (FNIP1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
[pSK467] pLJC5 HA-Depdc5

Plasmid

#109343

Purpose

Lenti-viral expression of HA-tagged wildtype Depdc5

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

HA

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-HA-C12orf66

Plasmid

#87048

Purpose

mammalian expression of C12orf66

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

C12orf66 (C12orf66 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJC5-Flag-SAMTOR

Plasmid

#102420

Purpose

3rd generation lentiviral vector expressing FLAG SAMTOR in mammalian cells driven by UBC promoter

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

SAMTOR (BMT2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

UBC

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
mSin1 shRNA #1

Plasmid

#13483

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

Insert

mSin1 (MAPKAP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Nov. 22, 2006

Availability

Academic Institutions and Nonprofits only
pRK5 CASTOR1-Flag Cterm

Plasmid

#84488

Purpose

overexpression of CASTOR1 with Flag tag

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR1 (GATSL3 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
pRK5-HA-ITFG2

Plasmid

#87045

Purpose

mammalian expression of ITFG2

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

ITFG2 (ITFG2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
mSin1 shRNA #2

Plasmid

#13484

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

Insert

mSin1 (MAPKAP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Nov. 22, 2006

Availability

Academic Institutions and Nonprofits only
[pSK468] pLJC5 Flag-Depdc5

Plasmid

#109337

Purpose

Lenti-viral expression of Flag-tagged wildtype Depdc5

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

FLAG

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Ef1a-DIO-VMAT2-WPRE-pA

Plasmid

#39339

Depositor

Bernardo Sabatini

Article

Tritsch et al Nature. 2012 Oct 11;490(7419):262-6. doi

Insert

Slc18a2 (Slc18a2 Mouse)

Use

AAV; R

Mutation

Myc tag inserted in the first lumenal loop betwee…

Available Since

Oct. 9, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-TKFC in pLJM60

Plasmid

#154206

Purpose

Lentiviral vector for stable expression of TKFC

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

TKFC (DAK Human)

Use

Lentiviral

Tags

N terminal FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-FLEX-FLIM-AKART391A

Plasmid

#60446

Purpose

Expresses the non-phosphorylatable point mutant (T391A) of FLIM-AKAR in a Cre dependent manner in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

FLIM-AKART391A

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

changed Threonine 391 to Ananine

Promoter

CAG

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-HA-HBXIP

Plasmid

#42328

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

HBXIP (LAMTOR5 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV, SP6

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-FLAG-Sestrin3

Plasmid

#72592

Purpose

overexpression, codon optimized for mammalian expression

Depositor

David Sabatini

Article

Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

Insert

SESN3 (SESN3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized for mammalian expression, see dep…

Promoter

CMV

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-Ef1a-DO-NpHR3.0-eYFP-WPRE-pA

Plasmid

#37087

Purpose

Expresses NpHR3.0-eYFP in cells lacking Cre (Cre-Off), for optogenetic inhibition

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

NpHR3.0-EYFP

Use

AAV and Cre/Lox; Cre-off

Tags

EYFP

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPRv1_UQCRC2

Plasmid

#177982

Purpose

lentiviral vector expressing Cas9 and a sgRNA targeting UQCRC2

Depositor

David Sabatini, Jessica Spinelli

Article

Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

Insert

sgRNA targeting UQCRC2 (UQCRC2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

U6

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-Flag CASTOR1 I280A

Plasmid

#84495

Purpose

overexpression of CASTOR1 I280A mutant

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR1 I280A (GATSL3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

I280A

Promoter

CMV

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
pMXs_FLAG-MCART2

Plasmid

#133250

Purpose

Express FLAG-tagged MCART2 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

MCART2 (SLC25A52 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

codon-optimized for expression in human cells

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs_FLAG-GGC1

Plasmid

#133258

Purpose

Express FLAG-tagged GGC1 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

GGC1 (GGC1 Budding Yeast)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

codon-optimized for expression in human cells

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pWP1_mHAL

Plasmid

#102318

Purpose

expression of mouse cDNA of HAL refractory to the guide in plasmid 4

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

Hal (Hal Mouse)

Use

Lentiviral

Promoter

EF1alpha

Available Since

Feb. 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs_FLAG-NDT1

Plasmid

#133254

Purpose

Express FLAG-tagged NDT1 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

NDT1 (YIA6 Budding Yeast)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

codon-optimized for expression in human cells

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5 HA-GST-Presc-mAkt1 (wt)

Plasmid

#48805

Purpose

expresses wt Akt1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

Akt1 (Akt1 Mouse)

Tags

HA-GST-Presc

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2013

Availability

Academic Institutions and Nonprofits only
pRK5 CASTOR2-Flag Cterm

Plasmid

#84490

Purpose

overexpression of CASTOR2 with Flag tag

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR2 (GATSL2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
Luciferase-pcw107

Plasmid

#64648

Purpose

(control) when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

n/a

Use

Lentiviral

Promoter

PGK

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-Ef1a-DO-ChETA-TdTomato-WPRE-pA

Plasmid

#37756

Purpose

Expresses ChETA-TdTomato in cells lacking Cre (Cre-Off), for optogenetic excitation

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

ChETA-TdTomato

Use

AAV and Cre/Lox; Cre-off

Tags

TdTomato

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-hCas9-T2A-mStrawberry

Plasmid

#187224

Purpose

Expresses human Cas9 nuclease and mStrawberry via T2A linker under control of a CMV promoter.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

Cas9

Use

CRISPR

Tags

Inserted T2A-mStrawberry at 3' terminal of M…

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs_FLAG-MCART6

Plasmid

#133251

Purpose

Express FLAG-tagged MCART6 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

MCART6 (SLC25A53 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

codon-optimized for expression in human cells

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CMV-FRT-myrSNAP-2A-H2BeGFP-WPRE

Plasmid

#102986

Purpose

AAV expressing membrane-targeted SNAP protein and nuclear-targeted eGFP after FLP-mediated recombination

Depositor

Marco Tripodi

Article

Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

Inserts

membrane-targeted SNAP protein after FLP-mediated recombination
Nuclear-targeted eGFP after FLP-mediated recombination

Use

AAV; Adeno associated viral vector

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Ef1a-DO-ChETA-EYFP-WPRE-pA

Plasmid

#37086

Purpose

Expresses ChETA-EYFP in cells lacking Cre (Cre-Off), for optogenetic excitation

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

ChETA-YFP

Use

AAV and Cre/Lox; Cre-off

Tags

EYFP

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM1 p18 mito

Plasmid

#26638

Depositor

David Sabatini

Article

Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

Insert

p18 mito (LAMTOR1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Oct. 26, 2010

Availability

Academic Institutions and Nonprofits only

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