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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,244 results for: sgRNA

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Showing: 221 - 240 of 9244 results

  3. pX330A-nBRD4/PITCh

    Plasmid
    #91794
    Purpose
    PITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4
    Depositor
    James Bradner, Behnam Nabet
    Publication
    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    chicken β-actin promoter
    Available since
    March 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    91794-plasmid-map-sequence-id-429087

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  7. pSpCas9n(BB)-2A-Puro (PX462) V2.0

    Plasmid
    #62987
    Purpose
    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)
    Depositor
    Feng Zhang
    Publication
    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9n-2A-Puro
    Use
    CRISPR
    Tags
    2A-Puro and 3XFLAG
    Expression
    Mammalian
    Mutation
    Cas9 D10A nickase mutant
    Available since
    March 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62987-plasmid-map-sequence-id-435258

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  16. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

    Plasmid
    #64219
    Purpose
    Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked via T2A to BFP linked to the Ad4 E1B55K gene via P2A
    Depositor
    Ralf Kuehn
    Publication
    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    sgRNA targeting ROSA26-1
    Use
    CRISPR
    Tags
    3xFLAG, NLS, and T2A-BFP-P2A-E1B
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available since
    May 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    64219-plasmid-map-sequence-id-107960

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  19. BIC-Gag-CAS9

    Plasmid
    #119942
    Purpose
    encodes a GAG (F-MLV)-CAS9(sp) fusion. Allows the production of GAG-CAS9 Virus like particles from producer cells in association with over expressed gRNA(s) and appropriate envelopes.
    Depositor
    Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci
    Publication
    Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GAG FMLV fused to spCAS9
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    hCMV
    Available since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    119942-plasmid-map-sequence-id-235075

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Showing: 221 - 240 of 9244 results