163,983 results
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Plasmid#175330PurposeProduction of HaloTag fusion proteinDepositorInsertENO1 (ENO1 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGT-Kh2
Plasmid#122587PurposeMAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)DepositorInsertbeta-lactamase/sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-K298M
Plasmid#140314PurposeExpresses HA-tagged human Src-K298M in mammalian cells for structure-function studiesDepositorInsertSrc mutant (SRC Human)
UseTags2X HAExpressionMammalianMutationK298MPromoterCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Ddo_piwi-1
Plasmid#107628PurposeIn situ hybridization and RNAi studies in planariansDepositorInsertpiwi-1
UseRNAi; In situ hybridizationTagsExpressionMutationPromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
PIA586
Plasmid#104399Purposevector for expression of single E.coli RNAP subunitDepositorInsertrpoD
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pMSCV-NLS-CBRed-d2-hygro
Plasmid#139196Purposenon-ERK dependent control plasmid for pMSCV-NLS-CBGreen-FIRE-puroDepositorInsertCBRed
UseLuciferase and RetroviralTagsd2 PEST domain and nuclear localization signal (N…ExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-Fos
Plasmid#131593Purposedoxycycline-inducible expression of mouse c-Fos in mammalian cellsDepositorInsertFBJ osteosarcoma oncogene (Fos Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE-mCMVAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDmDERusk
Plasmid#213702PurposeVector for red-light-induced bacterial gene expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-mCherry (AAV1)
Viral Prep#114469-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-CaMKIIa-mCherry (#114469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-mCherry plasmid DNA. CamKIIa-driven expression of mCherry. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsmCherryAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FIRE-pHLy
Plasmid#170775PurposeRatiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cellsDepositorInsertLAMP1 (LAMP1 Human)
UseLentiviralTagsmCherry and mTFP1ExpressionMammalianMutation84 nucleotide Signal Sequence switched to the beg…PromoterCMVAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDmREDusk
Plasmid#213701PurposeVector for red-light-repressed bacterial gene expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC-TtCas6-4xcrRNA4.5
Plasmid#127764PurposeFor expression of T. thermophilus Csm complex in E. coli (crRNA)DepositorInsertsSynthetic CRISPR array containing four identical T. thermophilus spacers
Cas6A
UseTagsExpressionBacterialMutationCodon optimized for expression in E. coliPromoterT7Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PIK3CA-H1047R
Plasmid#116500PurposeLentiviral expression of PIK3CA H1047RDepositorInsertPIK3CA (PIK3CA Human)
UseLentiviralTagsExpressionMutationH1047RPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFUGW mCherry-KASH
Plasmid#131505PurposemCherry_KASH epression under hUBC promotor; serves as backbone for ORANGE lenti knock-inDepositorInsertmCherry_KASH
UseLentiviralTagsExpressionMammalianMutationPromoterhUBCAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
EB3N-VVDfast-mCl3-iLID
Plasmid#190165PurposeExpresses EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.DepositorInsertEB3N-VVDfast-mClover3-iLID
UseTags-VVDfast-mClover3-iLIDExpressionMammalianMutation1-189 aa from EB3PromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Flag-mCherry-Binder
Plasmid#179139PurposeBinder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).DepositorInsertSspB
UseTagsFLAG and mCherryExpressionMammalianMutationY11K/A15E, residue numbering from pdb: 1ou9PromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40
Plasmid#124603PurposeCre-dependent soma-localized ChrimsonR for optogenetic stimulationDepositorHas ServiceAAV9InsertSoma-localized ChrimsonR
UseAAVTagsmRuby2ExpressionMammalianMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP7s-WPRE (AAV PHP.eB)
Viral Prep#104487-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pGP-AAV-syn-jGCaMP7s-WPRE (#104487). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7s-WPRE plasmid DNA. Synapsin-driven GCaMP7s calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-K298M
Plasmid#140315PurposeExpresses Flag-tagged human Src-K298M in mammalian cells for structure-function studiesDepositorInsertSrc mutant (SRC Human)
UseTags2X FlagExpressionMammalianMutationK298MPromoterCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQCascade
Plasmid#140622PurposeExpresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and CRISPR RNADepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pF CAG luc-EGFP-cre puro
Plasmid#67503PurposeConstitutive expression of a firefly luciferase-enhanced green fluorescent protein-cre recombinase fusion protein in mammalian cellsDepositorInsertluciferase-EGFP-cre
UseCre/Lox, Lentiviral, and Luciferase ; Fluorescent…TagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pREDawn-StrR-MCS
Plasmid#188979PurposeFor red light-induced bacterial expression of gene of interest (StrR)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 (AAV9)
Viral Prep#124603-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 (#124603). In addition to the viral particles, you will also receive purified pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 plasmid DNA. EF1a-driven, Cre-dependent expression of ChrimsonR fused to mRuby2 fluorophore and targeted to neuronal soma for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsmRuby2 (Cre-dependent)Available sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICH41421
Plasmid#50339DepositorInsert3'UTR, polyadenylation signal/terminator, nos (A. tumefaciens)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hChR2(H134R)-EYFP
Plasmid#26973PurposeAAV expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic excitationDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, and AAV9InserthChR2(H134R)
UseAAVTagsEYFPExpressionMammalianMutationH134RPromoterhSynAvailable sinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRwB
Plasmid#48734PurposedsRed plasmidDepositorInsertdsRed-Express
UseTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
C098
Plasmid#174345PurposeTemplate for making Nluc mRNA reporter with pA139.DepositorInsertNanoLuc luciferase
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceSept. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CamKII 0.4.Cre.SV40 (AAV1)
Viral Prep#105558-AAV1PurposeReady-to-use AAV1 particles produced from pENN.AAV.CamKII 0.4.Cre.SV40 (#105558). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII 0.4.Cre.SV40 plasmid DNA. CamKII-driven Cre expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsNoneAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-ChRmine-mScarlet-WPRE
Plasmid#130990PurposeAAV vector to drive the expression of ChRmine-mScarlet under the control of CamKIIa promoterDepositorInsertChRmine
UseAAVTagsmScarletExpressionMutationPromoterCaMKIIaAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only