We narrowed to 49,526 results for: ang

1. pGP-CAG-iGABASnFR2-WPRE-bGH-polyA

   Plasmid

   #218868

   Purpose

   Mammalian expression of improved GABA sensor (positive change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Insert

   iGABASnFR2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S99A F102Y F104Y L178S

   Promoter

   CAG

   Available since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3-EKAR4

   Plasmid

   #174437

   Purpose

   Improved cyan/yellow FRET-based ERK kinase activity reporter.

   Depositor

   Jin Zhang

   Article

   Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

   Insert

   EKAR4
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti-TetO-PM

   Plasmid

   #197387

   Purpose

   To establish all-in-one TetO-inducible cell line for Pdx1-Mafa expression (Doxycycline induced; rtTA included; Blasticidin resistant)

   Depositor

   Qiao Zhou

   Article

   Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

   Insert

   PDX1-T2A-MAFA
   

   Use

   Lentiviral; Teto

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Cas9-2A-Cre

   Plasmid

   #68468

   Purpose

   co-expressing Cas9 and Cre recombinase

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

   Insert

   Cas9 and Cre recombinase
   

   Use

   CRISPR

   Tags

   P2A-Cre-Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDONR223_Luciferase

   Plasmid

   #25894

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Insert

   Luciferase
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti-CaMKIIa-hChR2(H134R)-mCherry-WPRE

   Plasmid

   #20943

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by CaMKIIa promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   CaMKIIa

   Available since

   July 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AAV-phsyn-FLEX-KASH-EGFP-U6-ShGmeb1

   Plasmid

   #129706

   Purpose

   Express cre-dependent KASH-EGFP and Gmeb1 shRNA

   Depositor

   Yi Zhang

   Article

   Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

   Insert

   KASH-EGFP
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGP-AAV-syn-iGABASnFR2n-WPRE

   Plasmid

   #218875

   Purpose

   AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Insert

   iGABASnFR2n
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   S99A F104H R168P

   Promoter

   Synapsin

   Available since

   May 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUAST td tomato Rab5 WT

   Plasmid

   #58446

   Purpose

   expression of drosophila td tomato Rab5

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab5 (Rab5 Fly)
   

   Use

   Tags

   td tomato

   Expression

   Insect

   Mutation

   Promoter

   GAL4

   Available since

   July 21, 2014

   Availability

   Academic Institutions and Nonprofits only

10. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EYFP

    Plasmid

    #167898

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHDR-USR-eGFP

    Plasmid

    #208830

    Purpose

    HDR Universal Surrogate Reporter with a sgRNA and consistently expressed eGFP but without Cas9. The selective PuroR reporter gene was designed to be repaired only by the sgRNA/Cas9-triggered HDR.

    Depositor

    Zhiying Zhang

    Article

    Yan et al Mol Ther Nucleic Acids. 2020 Mar 6;19:77

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, U6

    Available since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Nanog-2A-mCherry

    Plasmid

    #48680

    Purpose

    For knock-in donor vector by CRISPR/CAS system

    Depositor

    Rudolf Jaenisch

    Article

    Yang et al Cell. 2013 Aug 27. pii: S0092-8674(13)01

    Insert

    Nanog (Nanog Mouse)
    

    Use

    CRISPR

    Tags

    Cherry

    Expression

    Mutation

    Promoter

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA-Flag-Kdm6b(1025-End)-pA

    Plasmid

    #100278

    Purpose

    In vitro transcription template (T7 promoter) for Kdm6b catalytic domain active form.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nature. 2017 Jul 19. doi: 10.1038/nature

    Insert

    Kdm6b (Kdm6b Mouse)
    

    Use

    Tags

    Flag epitope tag

    Expression

    Mammalian

    Mutation

    catalytic domain (1025aa-End)

    Promoter

    T7

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. U6-5' MS2-CircRNA

    Plasmid

    #213750

    Purpose

    For circular RNA-mediated prime editor using U6-5' MS2-CircRNA in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    5' ribozyme, 5' ligation sequences, 5' MS2, 3' ligation sequences, 3' ribozyme
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pREDIT_MCP-RecT

    Plasmid

    #164803

    Purpose

    mammalian-optimized expression of SSAP RecT fused to MCP (for MS2 aptamer), pLenti-EF1A-MCP-EXTEN-RecT-NLS

    Depositor

    Le Cong

    Article

    Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

    Insert

    RecT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

    Plasmid

    #42337

    Purpose

    Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_mcherry

    Plasmid

    #167902

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NAX_CAGGS_CAS9_m4_VPR_KanR neo

    Plasmid

    #167885

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pmiR-report-hVEGFA

    Plasmid

    #112553

    Purpose

    3'UTR reporter assay

    Depositor

    Shusheng Wang

    Article

    Zhou et al Mol Ther. 2016 Oct;24(10):1823-1835. doi

    Insert

    VEGFA (VEGFA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMXs-KDM4D

    Plasmid

    #188234

    Purpose

    Express human KDM4D in mammalian cells

    Depositor

    Young Tang

    Article

    Su et al Int J Mol Sci. 2021 Sep 28;22(19). pii:

    Insert

    Lysine demethylase 4D (KDM4D Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSUMO His6 SUMO MYC3

    Plasmid

    #177848

    Purpose

    Bacterial Expression of MYC3

    Depositor

    Karsten Melcher

    Article

    Zhang et al Nature. 2015 Sep 10;525(7568):269-73. do

    Insert

    MYC3 (MYC3 Mustard Weed)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCAS1yl

    Plasmid

    #73226

    Purpose

    Constitutive expression of Cas9 and sgRNA in Yarrowia lipolytica cells

    Depositor

    Sheng Yang

    Article

    Gao et al J Ind Microbiol Biotechnol. 2016 Aug;43(

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR; In y. lipolytica

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    unknown

    Available since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PB-3XpolyA-3XPC-miniCMV-mCherry-WPRE-insulator

    Plasmid

    #168291

    Purpose

    optimized miniCMV (OminiCMV)

    Depositor

    Hui Yang

    Article

    Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    miniCMV

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-hSyn1-EGFP-P2A-EGFPf-WPRE-HGHpA

    Plasmid

    #74513

    Purpose

    AAV vector that use human synapsin-1 promoter to drive the expression of EGFP and membrane-targeted EGFPf linked by self-cleaving P2A peptide.

    Depositor

    Guoping Feng

    Article

    Zhang et al J Neurosci. 2016 Feb 17;36(7):2247-60. d

    Insert

    EGFP-p2A-EGFP-f
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAcCas12nHS_ABE_D3_empty

    Plasmid

    #203809

    Purpose

    Plasmid containing a CMV-driven NLS-dAcCas12n(D240)-ABE-Design3-NLS cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion).

    Depositor

    Quanjiang Ji

    Article

    Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

    Insert

    ABE_D3
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. E407V Nuclear Myosin I YFP

    Plasmid

    #60242

    Purpose

    Expresses a E407V mutant of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding and motor activities

    Depositor

    Primal de Lanerolle

    Article

    Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

    Insert

    Nuclear Myosin 1 (Myo1c Mouse)
    

    Use

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Mutation

    E407V

    Promoter

    Available since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

27. pREDIT_Cas9-MS2-BB_BbsI

    Plasmid

    #164802

    Purpose

    REDIT backbone for wildtype Cas9, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9-T2A-EBFP

    Depositor

    Le Cong

    Article

    Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEN-L1-PJ23119-PplpT-sfGFP-TrrfB-BsaI-Scaf-L2

    Plasmid

    #196250

    Purpose

    Plasmid for cloning of a CRISPR-Cas9 guide RNA gene under promoter PJ23119

    Depositor

    Bing Yang

    Article

    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

    Insert

    attL1-GlpT-scGFP-attL2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119 promoter

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pmiHiFiCas9

    Plasmid

    #164832

    Purpose

    miHiFiCas9 expressing plasmid

    Depositor

    Eugene Chen

    Article

    Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:

    Insert

    miHiFiCas9 (contains Brex27 at sequence 5031-5138).
    

    Use

    CRISPR

    Tags

    FLAG and NLS

    Expression

    Mammalian

    Mutation

    change R 691 to A

    Promoter

    CMV

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLAC

    Plasmid

    #45072

    Purpose

    lacritin-intein fusion plasmid coding for intact mature lacritin without signal peptide

    Depositor

    Gordon Laurie

    Article

    Wang et al J Cell Biol. 2006 Aug 28;174(5):689-700.

    Insert

    Lacritin (LACRT Human)
    

    Use

    Protein expression vector

    Tags

    Intein

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMW41: pET-His-MBP-SUMO-R2Bm-SII

    Plasmid

    #209290

    Purpose

    Expresses the R2Bm ORF in E. coli

    Depositor

    Feng Zhang

    Article

    Wilkinson et al Science. 2023 Apr 21;380(6642):301-308.

    Insert

    R2
    

    Use

    Tags

    His, MBP, StrepII, and bdSUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pREDIT_Cas9n-MS2-BB_BbsI

    Plasmid

    #164804

    Purpose

    REDIT backbone for nickase Cas9n, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9n(D10A)-T2A-EGFP

    Depositor

    Le Cong

    Article

    Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3-Akt-Scramble

    Plasmid

    #175014

    Purpose

    Negative-control construct for Akt-STOPS.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    mCherry-Akt-Scramble
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. NAX_CAGGS_CAS9_m4_VPR_mcherry

    Plasmid

    #167886

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

35. pCMV-BE-VQRCas9

    Plasmid

    #101740

    Purpose

    expression of an optimized Cas9 for base-editing in zebrafish

    Depositor

    Shuo Lin

    Article

    Zhang et al Nat Commun. 2017 Jul 25;8(1):118. doi: 1

    Insert

    rAPOBEC1-XTEN-zCas9(VQR)-UGI-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, VQR mutations

    Promoter

    CMV

    Available since

    Jan. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pM122: pAAV-EFS-CasRx

    Plasmid

    #166870

    Purpose

    AAV vector for expressing CasRx

    Depositor

    Guei-Sheung Liu

    Article

    Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

    Insert

    RfxCas13d
    

    Use

    AAV and CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3-H2A-Akt-STOPS

    Plasmid

    #175010

    Purpose

    Akt-STOPS targeted to the nucleus via histone H2A.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    H2A-mCherry-Akt-STOPS
    

    Use

    Tags

    Histone H2A and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. AAV-phsyn-FLEX-KASH-EGFP-U6-shRNA

    Plasmid

    #129708

    Purpose

    Express cre-dependent KASH-EGFP and scramble shRNA

    Depositor

    Yi Zhang

    Article

    Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

    Insert

    KASH-EGFP
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-EF1a-Flp-DOG-NW

    Plasmid

    #75469

    Purpose

    Flp recombinase dependent on GFP (Flp-DOG) for rAAV production and expression in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

    Has Service

    AAV1

    Insert

    Flp-DOG
    

    Use

    AAV and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAcCas12nHS_RV6_empty

    Plasmid

    #203811

    Purpose

    Plasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.

    Depositor

    Quanjiang Ji

    Article

    Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

    Insert

    AcCas12nHS_RV6
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pLenti-CaMKIIa-hChR2(H134R)-EYFP-WPRE

    Plasmid

    #20944

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by CaMKIIa promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

42. pYJ34-PB-CRISPR-BANCR-E1-gRNA

    Plasmid

    #131077

    Purpose

    long non-coding RNA BANCR knock out

    Depositor

    Xiaojun Lian

    Article

    Jiang et al Bioact Mater. 2022 Feb 7;14:313-320. doi

    Insert

    BANCR Exon1 gRNA (BANCR Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. IBMc396

    Plasmid

    #161954

    Purpose

    Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase N-lobe fused to acVHH

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pDONR223_HcRed

    Plasmid

    #25892

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Insert

    hcRed
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pcDNA3-Nuc-ExRai-AMPKAR

    Plasmid

    #192450

    Purpose

    ExRai-AMPKAR targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    ExRai-AMPKAR-NLS
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. Hy_pMT Flag-UAP56 WT

    Plasmid

    #110125

    Purpose

    Expresses Flag-tagged human UAP56 (DDX39B)

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    UAP56 (DDX39B Human)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. LRP6-m5-pCS2-VSVG

    Plasmid

    #27283

    Depositor

    Xi He

    Article

    Tamai et al Mol Cell. 2004 Jan 16. 13(1):149-56.

    Insert

    low density lipoprotein receptor-related protein 6 (LRP6 Human)
    

    Use

    Xenopus injection

    Tags

    VSVG

    Expression

    Mutation

    Changed S/T of all five PPP(S/T)P motifs to Alani…

    Promoter

    Available since

    March 3, 2011

    Availability

    Academic Institutions and Nonprofits only

48. S1-Fc

    Plasmid

    #164220

    Purpose

    Expresses soluble, dimeric Fc-His fusion protein for the original SARS-CoV2 Spike S1 region, along with dTomato reporter

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

    Insert

    Spike S1 domain (S SARS-CoV)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pcDNA3-AktAR2-NES

    Plasmid

    #175011

    Purpose

    Cytosol-targeted AktAR2.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    AktAR-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pGP-AAV-CAG-iGABASnFR2n-WPRE

    Plasmid

    #218884

    Purpose

    AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2n
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    S99A F104H R168P

    Promoter

    CAG

    Available since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge