We narrowed to 9,772 results for: Tec;

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mNeongreen-2xrGBD

Plasmid

#129624

Purpose

Visualization of active RhoA

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

mNeongreen-2xrGBD

Expression

Mammalian

Promoter

CMVdel

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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ciCas9_pcDNA5

Plasmid

#100550

Purpose

Expresses ciCas9 in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

ciCas9

Use

CRISPR; Frt, flp-in

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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H2B-GFP-AsLOV2-NES27 (pDN158)

Plasmid

#72659

Purpose

Constitutively nuclear variant of a very strong, photocaged NES (AsLOV2-NES 27) for blocking endogenous, CRM-1-dependent nuclear export

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Insert

H2B-GFP-AsLOV2-NES27

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-attBr-ZsGreen-attP

Plasmid

#51265

Purpose

Bxb1-responsive fluorescent reporter plasmid containing reverse complementary ZsGreen flanked by Bxb1 attB/P recognition sites in head-to-head orientation

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

attBr-ZsGreen-attP

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

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pQC membrane CFP IX

Plasmid

#37336

Depositor

Connie Cepko

Article

Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

Insert

membrane CFP

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pGFP-hPMLIII

Plasmid

#181903

Purpose

Fluorescently tagged PMLIII

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

hPMLIII (PML Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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PEX3*-SBP-GFP

Plasmid

#120174

Purpose

Expresses a chimera of the peroxisomal-targeting signal of PEX3, SBP tag and GFP (fluorescent tag)

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

Peroxisomal biogenesis factor 3 (PEX3 Human)

Tags

SBP and eGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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G-GECO1-Orai3

Plasmid

#73563

Purpose

Green fluorescent reporter of Orai3-associated calcium influx

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Insert

G-GECO1-Orai3 (ORAI3 Human, Synthetic)

Tags

G-GECO1

Expression

Mammalian

Promoter

CMV Immdeiate Early

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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dmBACCS2-IRES-dOrai

Plasmid

#67627

Purpose

Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, and dOrai

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2-IRES-dOrai

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pHCMV-VSVgSS-15F11-TM

Plasmid

#207317

Purpose

Functional incorporation of 15F11 scFv (anti-HA) into envelope of virions

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

15F11 ScFv

Expression

Mammalian

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ155

Plasmid

#69306

Purpose

Gateway entry vector with AteCas9D10A

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

AteCas9D10A (Arabidopsis codon optimized)

Use

CRISPR

Mutation

D10A mutation; NLS fusion

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pStr-KDEL-IRES-ST-mCherry

Plasmid

#110727

Purpose

Expresses an Str-KDEL Hook for the RUSH system and separately Sialyltransferase-mCherry

Depositor

David Stephens

Article

McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.20180

Inserts

Str-KDEL
STase sialyltransferase (ST6GAL1 Human)

Tags

Str Streptavidin binding peptide (SBP) and mCherr…

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV and IRES

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pEB1N-LZ-LOV2(wt)

Plasmid

#107614

Purpose

N-terminal half of π-EB1 with wild-type LOV2, no fluorescent tag

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE1 (MAPRE1 Human)

Tags

A. sativa phototropin 1 LOV2 domain and GCN4 leuc…

Expression

Mammalian

Mutation

EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…

Promoter

CMV

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB-Zeo

Plasmid

#78352

Purpose

Expresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

Depositor

Michael Elowitz

Article

Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

Insert

H2B-mCherry-T2A-rTetR-KRAB

Use

Synthetic Biology

Tags

rTetR

Expression

Mammalian

Promoter

pEF alpha

Available Since

July 7, 2016

Availability

Academic Institutions and Nonprofits only

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OPA1-isoform5

Plasmid

#70177

Purpose

Mammalian expression of hsOPA1 isoform 5

Depositor

David Chan

Article

Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

Insert

hsOPA1 isoform 5 (OPA1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

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His-MBP human SPOP (aa 28-337)

Plasmid

#52294

Purpose

bacterial expression of human SPOP (aa 28-337) fused to His-MBP

Depositor

Brenda Schulman

Article

Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

Insert

SPOP (SPOP Human)

Tags

His-MBP and TEV

Expression

Bacterial

Promoter

T7

Available Since

April 11, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 hSlp2-a

Plasmid

#40047

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp2-a (SYTL2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pDisplay FLIPE-10u

Plasmid

#13546

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207R

Tags

ECFP and Venus

Expression

Mammalian

Mutation

A207R in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-blueAKAR

Plasmid

#118470

Purpose

cpBFP-based single-color biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

blueAKAR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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[pSK468] pLJC5 Flag-Depdc5

Plasmid

#109337

Purpose

Lenti-viral expression of Flag-tagged wildtype Depdc5

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

FLAG

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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