174,033 results

1. pGL3Luc-5XNF-kappaB

   Plasmid

   #185695

   Purpose

   Reporter plasmid with a double-strand oligonucleotides that contain five repeats of NF-kappaB binding consensus sequence (5'-GGGGAATTTCC-3').

   Depositor

   Esther López-Bayghen

   Article

   Hernandez-Melchor et al ASN Neuro. 2022 Jan-Dec;14:1759091422111

   Insert

   NF-kappaB (NFKB1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   NF-kappaB binding site

   Available Since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLEX305_FKBP12F36V-SHOC2

   Plasmid

   #134522

   Purpose

   Lentiviral plasmid expressing SHOC2 with N-term FKBP12(F36V) tag

   Depositor

   Andrew Aguirre

   Article

   Sulahian et al Cell Rep. 2019 Oct 1;29(1):118-134.e8. d

   Insert

   SHOC2 (SHOC2 Human)
   

   Use

   Lentiviral

   Tags

   FKBP12F36V-2xHA

   Expression

   Mammalian

   Mutation

   Silent mutations made at wobble base position at …

   Promoter

   hPGK

   Available Since

   Nov. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP-pBAD

   Plasmid

   #54762

   Purpose

   Localization: Protein Expression Vector , Excitation: 488, Emission: 507

   Depositor

   Michael Davidson

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   6xHis and EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pENN496_miniCRISPRcharm_dSaCas9

   Plasmid

   #220837

   Purpose

   mini CRISPRcharm expression in mammalian cells

   Depositor

   Jonathan Weissman

   Article

   Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

   Insert

   mini CRISPRcharm
   

   Use

   CRISPR

   Tags

   P2A-TagBFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   EFS

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. peGFP-N1-Cofilin

   Plasmid

   #186747

   Purpose

   Expresses wildtype GFP-tagged human cofilin in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

   Insert

   Cofilin-1 (CFL1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMD2.G-VSV-G-mut

   Plasmid

   #196508

   Purpose

   VSV-G mutant (K47Q, R354A)

   Depositor

   Howard Chang

   Article

   Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

   Insert

   VSV-G
   

   Use

   Tags

   NA

   Expression

   Mammalian

   Mutation

   K47Q, R354A

   Promoter

   CMV

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-D50 lamin A

   Plasmid

   #17653

   Depositor

   Tom Misteli

   Article

   Scaffidi et al Nat Med. 2005 Apr . 11(4):440-5.

   Insert

   D50 lamin A (LMNA Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Delta 50 (50 aa internal deletion in globular tai…

   Promoter

   CMV

   Available Since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEGFP-D50 lamin A

   Plasmid

   #17653

   Depositor

   Tom Misteli

   Article

   Scaffidi et al Nat Med. 2005 Apr . 11(4):440-5.

   Insert

   D50 lamin A (LMNA Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Delta 50 (50 aa internal deletion in globular tai…

   Promoter

   CMV

   Available Since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA-dCas9-p300 Core

   Plasmid

   #61357

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   CMV

   Available Since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pE-SUMO CRYAA

    Plasmid

    #80756

    Purpose

    Expression of human alpha A-crystallin in E. coli

    Depositor

    Larry David

    Insert

    CRYAA (CRYAA Human)
    

    Use

    Tags

    6xHIS and Smt3 SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST17-UnaG

    Plasmid

    #203485

    Purpose

    For recombinant protein production in Escherichia coli. His-tagged UnaG under the control of the T7 promoter.

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

    Insert

    UnaG
    

    Use

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a(+)_6xHIS-hHLA-A2 (MHC Class I heavy)-C-Tag

    Plasmid

    #187356

    Purpose

    Bacterial expression and refolding of gp100 pMHC

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Insert

    hHLA-A2 (MHC Class I heavy) with N-terminal 6xHIS tag, and C-terminal C-Tag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a(+)_beta2M (MHC Class I light)

    Plasmid

    #187357

    Purpose

    Bacterial expression and refolding of gp100 pMHC

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Insert

    beta2M (MHC Class I light)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMH-Halo tag

    Plasmid

    #154144

    Purpose

    Gateway compatible mammalian expression plasmid for halo tag fusion proteins

    Depositor

    Michael Huen

    Type

    Empty backbone

    Use

    Tags

    Halo tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV5 TBRI-HA with various GS domain mutations

    Plasmid

    #19162

    Depositor

    Joan Massague

    Insert

    transforming growth factor, beta receptor 1 (TGFBR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T204D (GS domain mutation)

    Promoter

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV5 TBRI-HA with various GS domain mutations

    Plasmid

    #19162

    Depositor

    Joan Massague

    Insert

    transforming growth factor, beta receptor 1 (TGFBR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T204D (GS domain mutation)

    Promoter

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. JpExpress404 PTEN-LONG

    Plasmid

    #49417

    Purpose

    Bacterial Expression Vector

    Depositor

    Ramon Parsons

    Article

    Hopkins et al Science. 2013 Jul 26;341(6144):399-402.

    Insert

    PTEN-Long (PTEN )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. PE-SUMO CRYBB1

    Plasmid

    #217675

    Purpose

    For expression of SUMO tagged human beta B1 crystallin

    Depositor

    Larry David

    Insert

    CRYBB1 (CRYBB1 Human)
    

    Use

    Tags

    6XHis and Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNTI935

    Plasmid

    #216638

    Purpose

    cpTurboID-PM (V5) Plasma membrane-localized cpTurboID, mammalian expression and lentivirus production

    Depositor

    Nicholas Ingolia

    Article

    Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

    Insert

    cpTurboID-PM
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHR_SFFV_LaG17_synNotch_TetRVP64

    Plasmid

    #79128

    Purpose

    Lentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch TetRVP64 Receptor (SFFV promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Insert

    LaG17 GFP nanobody synNotch TetRVP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. MP28444 – pFUSE2ss-aRD114_CHIg-mIgG2A

    Plasmid

    #110555

    Purpose

    pFUSE backbone with an IL2 signal sequence, expressing the variable heavy chain of anti-RD114 binder fused to murine IgG2A constant heavy chain.

    Depositor

    Martin Pule

    Article

    Mekkaoui et al J Gen Virol. 2019 Nov 8. doi: 10.1099/jg

    Insert

    Anti-RD114 heavy chain
    

    Use

    Tags

    mIgG2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF-1 alpha core promoter

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AAV-CMV-GFP

    Plasmid

    #67634

    Purpose

    AAV vector expressing GFP

    Depositor

    Connie Cepko

    Article

    Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-flex-taCasp3-TEVp

    Plasmid

    #45580

    Depositor

    Jim Wells, Nirao Shah

    Article

    Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

    Has Service

    AAV1 and AAV5

    Inserts

    Caspase 3 (CASP3 Synthetic, Human)
    Tobacco Etch Virus protease
    

    Use

    AAV; Adeno associated viral vector

    Tags

    Expression

    Mutation

    Linker replaced with a TEV protease cleavage site

    Promoter

    EF1a

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLenti.PGK.LifeAct-GFP.W

    Plasmid

    #51010

    Purpose

    Lentivector that expresses LifeAct-GFP from internal human PGK promoter

    Depositor

    Rusty Lansford

    Insert

    LifeAct-GFP
    

    Use

    Lentiviral

    Tags

    LifeAct

    Expression

    Mammalian

    Mutation

    Promoter

    human PGK

    Available Since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. MSCV-myc-CAR-2A-Thy1.1

    Plasmid

    #127890

    Purpose

    Expresses CART receptor along with tags.

    Depositor

    Anjana Rao

    Article

    Chen et al Nature. 2019 Mar;567(7749):530-534. doi:

    Inserts

    myc,
    CAR
    2A
    Thy1.1
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAG-VSVN

    Plasmid

    #64087

    Purpose

    Expression vector for Vesicular Stomatitis Virus Indiana strain nucleoprotein gene

    Depositor

    Ian Wickersham

    Insert

    VSV nucleoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MP28445 – pFUSE2ss-aRD114_CLIg-mIgK

    Plasmid

    #110556

    Purpose

    pFUSE backbone with an IL2 signal sequence, expressing the variable light chain of anti-RD114 binder fused to murine Kappa constant light chain.

    Depositor

    Martin Pule

    Article

    Mekkaoui et al J Gen Virol. 2019 Nov 8. doi: 10.1099/jg

    Insert

    anit-RD114 light chain
    

    Use

    Tags

    mKappa

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF-1 alpha core promoter

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET30-2-GAPDH

    Plasmid

    #83910

    Purpose

    stable overexpression

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    glyceraldehyde-3-phosphate dehydrogenase (GAPDH Human)
    

    Use

    Tags

    6x His tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. V. natriegens NC7

    Bacterial Strain

    #215356

    Purpose

    Vibrio natriegens with genomically integrated natural competence regulator, TfoX. For zero-capital molecular biology applications.

    Depositor

    Buz Barstow

    Article

    Specht et al PNAS Nexus. 2024 Feb 13;3(2):pgad444. do

    Bacterial Resistance

    None

    Available Since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

30. pDONR223_EGFR_p.769_770insASV

    Plasmid

    #82880

    Purpose

    Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

    Depositor

    Matthew Meyerson, Jesse Boehm, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    EGFR (EGFR Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    ASV insertion between AA 769 and 770

    Promoter

    None

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCFJ108 - pENTR221 - [cbr-unc-119(+)]

    Plasmid

    #200367

    Purpose

    cbr-unc-119 rescue gene for use as co-injection marker.

    Depositor

    Christian Froekjaer-Jensen

    Article

    AlHarbi et al MicroPubl Biol. 2023 Jun 22;2023:10.1791

    Insert

    [cbr-unc-119(+)]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    June 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. FUW

    Plasmid

    #14882

    Depositor

    David Baltimore

    Article

    Lois et al Science. 2002 Feb 1. 295(5556):868-72.

    Insert

    flap-Ub promoter-WRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. FUW

    Plasmid

    #14882

    Depositor

    David Baltimore

    Article

    Lois et al Science. 2002 Feb 1. 295(5556):868-72.

    Insert

    flap-Ub promoter-WRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMAZ-SK

    Plasmid

    #73962

    Purpose

    self-killing gRNA plasmid pMAZ-SK

    Depositor

    Alex Nielsen

    Article

    Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

    Insert

    gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available Since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. hCas9

    Plasmid

    #41815

    Purpose

    Expresses human codon optimized Cas9 nuclease for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    CMV

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCWXPGR-pTF-betaCatenin

    Plasmid

    #114281

    Purpose

    TET-inducible expression of a constitutively active form of the BetaCatenin protein

    Depositor

    Patrick Salmon

    Article

    Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

    Insert

    Catenin beta 1 (CTNNB1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    deletion of AA 1 to 45

    Promoter

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV-EF1α1.1-GFP

    Plasmid

    #122100

    Purpose

    AAV-mediated expression of GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Has Service

    AAV2

    Insert

    GFP
    

    Use

    AAV

    Tags

    NA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a（1.1kb short version)

    Available Since

    May 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. AAV-pgk-Cre (AAV Retrograde)

    Viral Prep

    #24593-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from AAV-pgk-Cre (#24593). In addition to the viral particles, you will also receive purified AAV-pgk-Cre plasmid DNA. Cre expression under the PGK promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons.

    Depositor

    Patrick Aebischer

    Promoter

    pgk

    Tags

    None

    Available Since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

39. pTetOFF SNCA-A53T

    Plasmid

    #215372

    Purpose

    All-in-one lentiviral construct for the dox repressible expression of the A53T mutant of alpha-synuclein.

    Depositor

    Franz-Ulrich Hartl

    Article

    Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

    Insert

    SNCA (SNCA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ala53Thr

    Promoter

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEGFP-C1-wtVHL

    Plasmid

    #13390

    Depositor

    Lucy Anderson

    Article

    Shiao et al Cancer Res. 2000 Jun 1. 60(11):2816-9.

    Insert

    von Hippel-Lindau (Vhl Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge