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169,450 results

Showing: 5041 - 5080 of 169450 results

  2. dCas9-miniTurbo

    Plasmid
    #219822
    Purpose
    EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)
    Depositor
    Philipp Maass
    Insert
    dCas9
    Use
    CRISPR
    Tags
    miniTurbo
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    EF1a
    Available Since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    219822-plasmid-map-sequence-id-482202

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  4. pAAV-hSynapsin1-FLEx-axon-GCaMP6s (AAV9)

    Viral Prep
    #112010-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSynapsin1-FLEx-axon-GCaMP6s (#112010). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-FLEx-axon-GCaMP6s plasmid DNA. Synapsin-driven, cre-dependent axon-targeted GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Lin Tian
    Article
    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.
    Promoter
    Syn
    Available Since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only

  7. Multiplex Yeast Toolkit (MYT)

    Plasmid Kit
    #1000000229
    Purpose
    The Multiplex Yeast Toolkit (MYT) is a collection of 96 plasmids for genetically engineering the yeast, Saccharomyces cerevisiae.
    Depositor
    Tom Ellis
    Available Since
    Nov. 9, 2023
    Availability
    Academic Institutions and Nonprofits only

  8. AAV-hSyn-mCherry-P2A-Cre-WPRE (AAV1)

    Viral Prep
    #107312-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from AAV-hSyn-mCherry-P2A-Cre-WPRE (#107312). In addition to the viral particles, you will also receive purified AAV-hSyn-mCherry-P2A-Cre-WPRE plasmid DNA. hSyn-driven expression of mCherry and Cre (physically separate). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hui Yang
    Article
    Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/
    Tags
    mCherry (physically separate, not a fusion protein)
    Available Since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only

  19. pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 (AAV9)

    Viral Prep
    #100835-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 (#100835). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Promoter
    CAG
    Available Since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only

  21. pCMV-T7-ABE8.20m-nSpG-P2A-EGFP (KAC1160)

    Plasmid
    #185916
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8.20m-nSpG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…
    Promoter
    CMV and T7
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185916-plasmid-map-sequence-id-367354

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  23. pSF4 TetCMV ATF4(5'UTR)-SunTag-Renilla-FKBP-Stop-24xMS2v5-CTE-polyA

    Plasmid
    #164615
    Purpose
    Expresses ATF4(5'UTR)-SunTag-Renilla mRNA reporter under tetracycline-inducible promoter. SunTag and Renilla luciferase are in frame with the main start codon of ATF4.
    Depositor
    Jeffrey Chao
    Article
    Mateju et al Cell. 2020 Dec 23;183(7):1801-1812.e13.
    Insert
    Renilla luciferase
    Tags
    24xMS2v5 (3'UTR) and SunTag
    Expression
    Bacterial and Mammalian
    Promoter
    TetCMV
    Available Since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    164615-plasmid-map-sequence-id-325006

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  28. pET29b-H6_Streptavidin_sfGFP

    Plasmid
    #124296
    Purpose
    His6-tagged Streptavidin GFP fusion protein
    Depositor
    Mark Arbing
    Insert
    His6 tag - TEV protease site - T7 tag - Streptavidin (15-159) - superfolder GFP
    Tags
    His6 and T7 tags
    Expression
    Bacterial
    Available Since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    124296-plasmid-map-sequence-id-240453

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  33. pCDH-EF1-Luc2-P2A-copGFP

    Plasmid
    #72485
    Purpose
    Express Luc2 and copGFP
    Depositor
    Kazuhiro Oka
    Insert
    Luc2-P2A-copGFP
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1
    Available Since
    Jan. 11, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72485-plasmid-map-sequence-id-132335

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  34. lenti MS2-P65-HSF1_Hygro

    Plasmid
    #61426
    Purpose
    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Insert
    MS2-P65-HSF1_2A_Hygro (HSF1 Human, Synthetic)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1A
    Available Since
    Dec. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    61426-plasmid-map-sequence-id-140565

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  35. Td-CBE-NW

    Plasmid
    #240623
    Purpose
    Cytidine base editor with narrowed editing window
    Depositor
    Tingting Jiang
    Article
    Valdez et al Nat Commun. 2025 Aug 30;16(1):8115. doi:
    Insert
    TadA-8e (E27R, N46L, N108Q, M151C, Q154Y)-linker-nCas9-P2A-UGI
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    TadA-8e (E27R, N46L, N108Q, M151C, Q154Y), Cas9-P…
    Available Since
    July 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    240623-plasmid-map-sequence-id-480852

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  39. p667-UBC-CDK12-V5-miniTurbo_IDG-K

    Plasmid
    #135243
    Purpose
    Gateway destination clone of CDK12 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    CDK12-V5-miniTurbo (CDK12 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135243-plasmid-map-sequence-id-269114

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  40. pLSC-5

    Plasmid
    #62889
    Purpose
    Lenti vector for expression of inducible split Cas9
    Depositor
    Feng Zhang
    Article
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Inserts
    SpCas9(574-1368)
    SpCas9(2-573)
    Use
    CRISPR and Lentiviral
    Tags
    FFBP12, FRB, NES PTK2, NLS SV40, and P2A
    Expression
    Mammalian
    Mutation
    *see comment below
    Promoter
    EFS
    Available Since
    March 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    62889-plasmid-map-sequence-id-105912

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Showing: 5041 - 5080 of 169450 results