We narrowed to 10,119 results for: transfer

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pAAV-hsyn-GRAB_ATP1.0mut

Plasmid

#167578

Purpose

Express the ATP-insensitive control sensor GRAB_ATP1.0mut in neurons

Depositor

Yulong Li

Article

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)

Use

AAV

Promoter

hSyn

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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TBG-Vector

Plasmid

#109396

Purpose

AAV-CRISPR knockout plasmid with Cre recombinase driven by TBG promoter for direct in vivo genome editing in mouse liver

Depositor

Sidi Chen

Article

Wang et al Sci Adv. 2018 Feb 28;4(2):eaao5508. doi:

Type

Empty backbone

Use

AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…

Expression

Mammalian

Promoter

TBG

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-BDNF-Myc

Plasmid

#124279

Purpose

Express Myc-tagged brain-derived neurotrophic factor (BDNF)

Depositor

Baoji Xu

Article

Xie et al Cell Metab. 2019 Jan 11. pii: S1550-4131

Insert

BDNF (Bdnf Mouse)

Use

AAV

Tags

Myc

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-sL7-Cre-HA-WPRE

Plasmid

#204488

Purpose

For production of driver AAV virus for Purkinje cell-specific expression

Depositor

Samuel Wang

Article

Chen et al Nature. 2022 May;605(7911):722-727. doi:

Insert

Cre

Use

AAV, Cre/Lox, and Mouse Targeting

Expression

Mammalian

Promoter

sL7

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-double floxed-ClopHensorN-WPRE-HGHpA

Plasmid

#193728

Purpose

Cre-activated AAV expression of ClopHensorN for measuring intracellular chloride and pH in genetically-defined cell types

Depositor

Colin Akerman

Article

Calin et al iScience. 2023 Mar 9;26(4):106363. doi:

Insert

ClopHensorN

Use

AAV

Tags

Strep-II (N terminal on insert)

Expression

Mammalian

Promoter

EF1a

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-eLACCO1.1

Plasmid

#168788

Purpose

Neuronal expression of fluorescent sensor for extracellular lactate

Depositor

Marie-Eve Paquet

Article

Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

Insert

eLACCO1.1

Use

AAV

Tags

ER export and GPI

Expression

Mammalian

Promoter

hSyn

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX(frt)-GCaMP6f-WPRE

Plasmid

#118273

Purpose

pAAV vector for flippase dependent GCaMP6f expression under the control of EF1a promoter

Depositor

Naoshige Uchida

Article

Menegas et al Nat Neurosci. 2018 Sep 3. pii: 10.1038/s

Insert

GCaMP6f

Use

AAV

Tags

6xHis (N terminal on insert), T7 epitope, and Xpr…

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSYN-bArrestin2-TevC-P2A-TdTomato-WPRE-bGHpA

Plasmid

#89873

Purpose

expresses iTango bArrestin2-TevC component and TdTomato bicistronically for AAV production

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

bArrestin2-TevC-P2A-TdTomato

Use

AAV

Tags

TdTomato

Expression

Mammalian

Mutation

R173H in TdTomato (Please see depositor comments …

Promoter

Human synapsin

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-GCaMP6s-WPRE-pGHpA

Plasmid

#67526

Purpose

"Fluorescent reporter for calcium imaging"

Depositor

Botond Roska

Article

Wertz et al Science. 2015 Jul 3;349(6243):70-4. doi:

Insert

GCaMP6s

Use

AAV

Tags

6xHis, T7 tag, and Xpress epitope

Expression

Mammalian

Promoter

EF1a

Available Since

July 23, 2015

Availability

Academic Institutions and Nonprofits only

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pyc126m

Plasmid

#158777

Purpose

ribosome-tagged GCaMP for soma localization

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

GCaMP6m-RPL10a

Use

AAV

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hGfap-R-CaMP1.07-WPRE-SV40

Plasmid

#164140

Purpose

AAV vector to express the red Ca2+ indicator R-CaMP1.07 in astrocytes.

Depositor

Hirokazu Hirai, Hajime Hirase

Article

Oe et al Nat Commun. 2020 Jan 24;11(1):471. doi:

Insert

R-CaMP1.07

Use

AAV

Promoter

hGFAP

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-pEF1a-EBFP-tTAn

Plasmid

#172122

Purpose

Constitutive expression of EBFP and rtTA-InteinN

Depositor

Chun Xu

Article

Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

Insert

EBFP, tTAn-InteinN

Use

AAV

Promoter

EF1a

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-eLACCO1.1

Plasmid

#168790

Purpose

Astrocyte expression of fluorescent sensor for extracellular lactate

Depositor

Marie-Eve Paquet

Article

Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

Insert

eLacco1.1

Use

AAV

Tags

ER export and GPI

Expression

Mammalian

Promoter

GFAP

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-ArchT-GFP

Plasmid

#28307

Purpose

Cre-dependent viral expression of ArchT-GFP for neuronal inhibition

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Insert

ArchT-GFP

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

GFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-FLInChR-mVenus

Plasmid

#119298

Purpose

FLinChR is the fusion signal sequence and transmembrane (TM) domain of Neurexin 1B-delta to ChR2E123T/T159C for inversion ChR2 to an optogenetic inhibitor

Depositor

Alla Karpova

Article

Brown et al Cell. 2018 Oct 16. pii: S0092-8674(18)31

Insert

Nx1BTM-FCS-ChR2ET/TC-mVenus

Use

AAV

Tags

mVenus

Promoter

CAG

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST

Plasmid

#109048

Purpose

Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence in a viral vector

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Has Service

AAV9

Insert

eGtACR1-ST

Use

AAV

Promoter

CAG

Available Since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-TVA-V5-RG

Plasmid

#119743

Purpose

Expresses TVA, V5, and RG in a cre dependent manner.

Depositor

Marie Carlén

Article

Ahrlund-Richter et al Nat Neurosci. 2019 Apr;22(4):657-668. do

Insert

TVA, V5, Rabies Glycoprotein

Use

AAV and Cre/Lox

Tags

V5

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pyc111s

Plasmid

#158781

Purpose

ribosome-tagged, Cre-dependent GCaMP for soma localization

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

GCaMP6s-RPL10a

Use

AAV

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-mCherry(LaM8) PAGER(TF)

Plasmid

#229999

Purpose

Expresses anti-mCherry PAGER(TF) (high reversibility) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

IL2SP-Aro6-LaM8-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4

Use

AAV

Expression

Mammalian

Mutation

V360A/R361A on KORD

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-U6sgp53-U6sgbbSapI-GFAPCre

Plasmid

#100275

Purpose

AAV-CRISPR mediated genome editing in glia / astrocytes

Depositor

Sidi Chen

Article

Chow et al Nat Neurosci. 2017 Aug 14. doi: 10.1038/

Type

Empty backbone

Use

AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…

Expression

Mammalian

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-SauriCas9-KKH-Puro

Plasmid

#135966

Purpose

Expresses SauriCas9-KKH and puromycin resistance genes, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV CMV-DIO-(mCherry-U6)-shRNA(scrambled)

Plasmid

#214733

Purpose

Expresses mCherry protein in infected and cre positive cells

Depositor

Félix Leroy

Article

de Leon Reyes et al Cell. 2023 Sep 14;186(19):4152-4171.e31.

Insert

anti-Crh shRNA (scrambled) / mCherry

Use

AAV

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pX601-AAV-CMV-NLS-ISTfu1-NLS-3xHA-bGHpA;U6-BbsI-reRNA

Plasmid

#205319

Purpose

All-in-one AAV vector expressing ISTfu1 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

humanized ISTfu1 TnpB

Use

AAV

Tags

3 x HA

Expression

Mammalian

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pX552-hsyn DIO GCaMP8f(with gRNA scaffold)

Plasmid

#199580

Purpose

Expresses GCaMP8f in a Cre-dependent manner

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

His-DIO GCaMP8f

Use

AAV, CRISPR, and Cre/Lox

Tags

6xHis

Expression

Mammalian

Promoter

hsyn

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-dDIO-vCre

Plasmid

#118029

Purpose

AAV vector for sparse and bright labeling in a cell-type specific manner

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

Insert

TRE-dDIO-vCre

Use

AAV

Mutation

See depositor comments below

Promoter

TRE

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-axon-GCaMP6s-P2A-mKate2

Plasmid

#112004

Purpose

For enriched expression of GCaMP6s in axons with cytosolic expression of mKate2

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has Service

AAV9

Insert

axon-GCaMP6s-P2A-mKate2

Use

AAV

Tags

GAP43 palmitoylation domain

Expression

Mammalian

Promoter

hSynapsin1

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-SCP1-dSa VPR mini.-2X snRP-1 Hbb

Plasmid

#99692

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Hbb, vector allows for strong activation of mouse Hbb, can be packaged and delivered as AAV

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9 and gRNA targeting Hbb (Hbb Synthetic)

Use

AAV

Tags

VPR mini

Mutation

dead Cas9

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.SV40.eGFP.SV40(polyA)

Plasmid

#195558

Purpose

Expresses eGFP under control of short GFAP promoter

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

eGFP

Use

AAV

Tags

No tags

Expression

Mammalian

Promoter

short GFAP

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-VEGF(Nb35) PAGER(TF)

Plasmid

#230000

Purpose

Expresses anti-VEGF PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

IL2SP-Aro6-Nb35-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4

Use

AAV

Expression

Mammalian

Mutation

V360A/R361A on KORD

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-linker-TlcnC

Plasmid

#114377

Purpose

expresses Flpo recombinase-dependent GtACR2-Kv2.1C-linker-TlcnC, which targets GtACR2 to the somatodendritic compartment. Messier et al found this hybrid construct was the most effective at targeting

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-Kv2.1C-linker-TlcnC (NEWENTRY )

Use

AAV

Tags

EYFP and Kv2.1C-linker-TlcnC

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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