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pLV-CMV-dnMCAK
Plasmid#205993PurposeOver-expression of GFP-dnMCAKDepositorInsertdnMCAK
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_STAT6_WT
Plasmid#81950PurposeGateway Donor vector containing STAT6, part of the Target Accelerator Plasmid Collection.DepositorInsertSTAT6 (STAT6 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL-FLKif3A
Plasmid#13742DepositorInsertmouse Kif3A (Kif3a Mouse)
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBHM2404
Plasmid#173441PurposepRS316-mNeonGreen-CBP-2XFLAG-NAT1, contains C. neoformans optimized mNeonGreen and NAT1 marker for use as PCR templateDepositorInsertmNeonGreen-CBP-2XFLAG-NAT1
UseTagsCBP-2xFLAGExpressionBacterial and YeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTSN6
Plasmid#131318PurposeIntegrating the full-length tetO array (from pLAU44) near the his-3 gene in N. crassaDepositorInsertFull-length tetO array (XbaI-NheI fragment of pLAU44)
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUCP30T-E2Crimson
Plasmid#78478PurposeBroad range bacterial fluorescence labelingDepositorInsertE2-Crimson
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBHM2406
Plasmid#173442PurposepRS316-mNeonGreen-CBP-2XFLAG-HYGB, contains C. neoformans optimized mNeonGreen and HYG marker for use as PCR templateDepositorInsertmNeonGreen-CBP-2XFLAG-HYGB
UseTagsCBP-2xFLAGExpressionBacterial and YeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAK_DR30_CasRx_GFP
Plasmid#134843PurposeAll in one overexpression of CasRx and guideRNADepositorInsertRfxCas13d
UseCRISPRTagsT2A EGFPExpressionMammalianMutationPromoterCAGAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
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Bmi1-overexpression
Plasmid#21577DepositorInsertBmi-1 (Bmi1 Mouse)
UseLentiviralTagsires-eGFPExpressionMammalianMutationPromoterAvailable sinceJune 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pT2-shP53
Plasmid#124261PurposeExpresses shRNA targeting P53. Construct has inverted repeats to be used in Sleeping beauty system.DepositorInsertshP53
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
478 p5Efli1ep
Plasmid#31160DepositorInsertchimeric fli1ep enhancer/promoter fragment
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceSept. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
413 pSG5L HA RB
Plasmid#10720DepositorInsertRB (RB1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-Twin-Strep_IRES-mRuby2
Plasmid#113885PurposeMammalian (inducible) protein expression, bicistronic expression of cytosolic mRuby2DepositorTypeEmpty backboneUseLentiviralTags3C-Twin-StrepExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-INPP5K
Plasmid#171950PurposeExpresses INPP5K in mammalian cellsDepositorInsertINPP5K (INPP5K Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
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MK01
Bacterial Strain#195090PurposeImproved E. coli strain created by knocking out the endogenous lacI gene with chloramphenicol acetyltransferase (cat) selection cassette in the parent strain BW27783 that overcome the all-or-none response of araBAD promoter (PBAD).DepositorBacterial ResistanceChloramphenicolAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
RFP-HA-DAT
Plasmid#90265PurposeExpresses RFP-HA-DAT in mammalian cellsDepositorInsertDAT (dopamine transporter) (SLC6A3 Human)
UseTagsExpressionMammalianMutationinserted HA tag (see YFP-HA-DAT)PromoterCMVAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBR.DT-A
Plasmid#35955DepositorInsertdiphtheria toxin A
UseBac recombineeringTagsExpressionMutationPromoterPGKAvailable sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMK782 Septin6-GFP
Plasmid#38296DepositorInsertSeptin 6 (Septin6 Mouse)
UseTagseGFPExpressionMammalianMutation22 amino acid linker sequence (added by PCR)PromoterCMVAvailable sinceNov. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]
Plasmid#227246PurposeAll-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cellDepositorInsertsTet-On 3G transactivator protein
Enhanced green fluorescent protein
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1α and pTRE3GAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
CiDr-VSP L223F-mCherry pcDNA3.1 (eVSP)
Plasmid#140892PurposeExpresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cells.DepositorUseTagsmCherryExpressionMammalianMutationchanged 223L to F in Dr-VSP (zebrafish TPTE)PromoterAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
prDNA spacer-LacOx50-20xMS2-Hyg
Plasmid#210219PurposeIntegrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.DepositorInserts50xLacO repeats derived from: PMID: 12864855.
24xMS2 repeats derived from Addgene plasmid # 31865
UseTagsExpressionMutationPromoterAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
p2706-pHAGE-EF1aL-YAP5SA-UBC-GFP
Plasmid#216475Purposelentiviral dual expression of YAP5SA and eGFPDepositorInsertsYAP5SA
eGFP
UseLentiviralTagsExpressionMutationS61A, S109A, S127A, S164A, S381APromoterEF1aL and UBCAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_CCFH_GP
Plasmid#207259PurposeMammalian expression of Crimean-Congo hemorrhagic fever virus GP proteinDepositorInsertCCFH_GP (CCFHV_sMgp1 Crimean-Congo hemorrhagic fever virus)
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRIM33-GFP
Plasmid#65399PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertTRIM33 (TRIM33 Human)
UseTagsEmGFPExpressionMammalianMutationnonePromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAF020 p3E-2A-mCerulean
Plasmid#195974PurposeGateway p3E 2A fluorophoreDepositorInsert2A-mCerulean
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcU6_3 sgRNA
Plasmid#92359PurposeChicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter.DepositorInsertchick U6.3 promoter and gRNA cloning cassette
UseCRISPRTagsExpressionMammalianMutationPromoterchick U6.3Available sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti GW V5 Eco/Dam hum LaminB1
Plasmid#182671PurposeEukaryotic expression vector with E.Coli Dam methylation fused to human Lamin B1. Used in DamID experiments to methylate DNA that interacts in the proximity of Lamin B1.DepositorInsertLamin B1 (LMNB1 Human)
UseLentiviralTagsE. Coli Dam and V5ExpressionMammalianMutationPromoterHSPAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only
