167,388 results

1. pLminP_Luc2P_RE22

   Plasmid

   #90364

   Purpose

   STAT6 - Stat6 gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Promoter

   STAT6

   Available Since

   Aug. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1_Lifeact-EGFP

   Plasmid

   #67303

   Purpose

   Fluorescent indicator for F-actin

   Depositor

   Moritoshi Sato

   Article

   Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

   Insert

   Lifeact-EGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLQ9926: pHR-PGK-SV40_NLS-dCasMINI-V4-VPR-c- Myc_NLS-mCherry-WPRE

   Plasmid

   #176269

   Purpose

   Expression of dCasMINI-V4-VPR-mCherry in mammalian cells

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   dCasMINI-V4-VPR-mCherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (AAV9)

   Viral Prep

   #104492-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (#104492). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7f-WPRE plasmid DNA. Syn-driven, Cre-dependent GCaMP7f calcium sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Promoter

   Syn

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

5. pBBR1k-con-GFPuv

   Plasmid

   #106386

   Purpose

   Constitutive trc promoter expressing gfpuv on BBR1 origin

   Depositor

   Brian Pfleger

   Article

   Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

   Insert

   GFPuv
   

   Expression

   Bacterial

   Promoter

   trc

   Available Since

   March 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQE-60 roGFP2-Orp1-His

   Plasmid

   #64976

   Purpose

   Bacterial expression of His-tagged roGFP2-Orp1

   Depositor

   Tobias Dick

   Article

   Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

   Insert

   Orp1 (HYR1 Budding Yeast)
   

   Tags

   His and roGFP2

   Expression

   Bacterial

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G

   Plasmid

   #167935

   Purpose

   Inducible CRISPRi expression

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   KRAB-dCas9-DHFR
   

   Use

   Lentiviral

   Promoter

   tetON

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET_RTX

   Plasmid

   #102787

   Purpose

   Expresses the Reverse Transcriptase gene

   Depositor

   Andrew Ellington

   Article

   Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

   Insert

   reverse transcription xenopolymerase
   

   Expression

   Bacterial

   Promoter

   T7 promoter + lac operator

   Available Since

   July 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Mouse CRISPRi sgRNA library Dolomiti in backbone XPR_050

   Pooled Library

   #1000000131

   Purpose

   Genome-wide CRISPR gRNA pooled library for inhibition of mouse genes

   Depositor

   David Root, John Doench

   Available Since

   July 13, 2018

   Availability

   Academic Institutions and Nonprofits only

10. pJMP1356

    Plasmid

    #119276

    Purpose

    for cloning new sgRNAs

    Depositor

    Carol Gross, Oren Rosenberg, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGP-AAV-syn-iGABASnFR2-WPRE

    Plasmid

    #218874

    Purpose

    AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Has Service

    AAV1 and AAV5

    Insert

    iGABASnFR2
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    S99A F102Y F104Y L178S

    Promoter

    Synapsin

    Available Since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLEX307-APN-blast

    Plasmid

    #158455

    Purpose

    Constitutive lentiviral expression of human APN.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    APN (ANPEP Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBK2044-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2

    Plasmid

    #223164

    Purpose

    2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB-MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    EF1a

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSNAPf-N1

    Plasmid

    #58187

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    SNAP tag

    Expression

    Mammalian

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. beta-catenin (S33A, S37A, T41A, S45A)-pcw107-V5

    Plasmid

    #64613

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    Ctnnb1 (Ctnnb1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    S33A, S37A, T41A, S45A

    Promoter

    PGK

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBR-Lent-STAT5A-CA

    Plasmid

    #170662

    Purpose

    Lentiviral expression of constitutively active STAT5A.

    Depositor

    Alexander Ruthenburg

    Article

    Richter et al Elife. 2021 Jul 15;10. pii: 64960. doi:

    Insert

    STAT5A (STAT5A Human)
    

    Use

    Lentiviral

    Mutation

    H298R and S710F

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-CD81-10

    Plasmid

    #55012

    Purpose

    Localization: Tetraspanin/Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    CD81 (CD81 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAS_4xMma PylT_FLAG-Mma PylRS AF

    Plasmid

    #140023

    Purpose

    Plasmid with 4xMmaPylT cassette and MmaPylRS AF for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y271A, Y349F

    Promoter

    EF1

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL3-rev-erbα

    Plasmid

    #186823

    Purpose

    Fluorescent reporter for rev-erbα expression

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    rev-erbα promoter
    

    Use

    Luciferase

    Promoter

    rev-erbα promoter

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSL1765 (pSPIN-R, R*MmeI)

    Plasmid

    #160734

    Purpose

    Single-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCAG-HA-Nrxn1beta AS4(-)

    Plasmid

    #59409

    Purpose

    Expression plasmid for HA-tagged mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]

    Depositor

    Peter Scheiffele

    Article

    Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

    Insert

    Nrxn1beta AS4(-) (Nrxn1 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCS2FA-H2B-mScarlet3

    Plasmid

    #224436

    Purpose

    Construct for labelling cell nuclei in mammalian cells using Histon protein 2B fused to mScarlet3

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    H2B-mScarlet3
    

    Expression

    Mammalian

    Promoter

    CMV & SP6

    Available Since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. pMX229

    Plasmid

    #23002

    Depositor

    Linda Wordeman

    Article

    Stumpff et al Dev Cell. 2008 Feb . 14(2):252-62.

    Insert

    Kif18A (KIF18A Human)
    

    Tags

    6His and EGFP

    Expression

    Mammalian

    Available Since

    Feb. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLV-EF1a-IRES-Neo

    Plasmid

    #85139

    Purpose

    lentiviral backbone

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. pcDNA3-myc-FLNa WT

    Plasmid

    #8982

    Depositor

    John Blenis

    Article

    Woo et al Mol Cell Biol. 2004 Apr . 24(7):3025-35.

    Insert

    Filamin A (FLNA Human)
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    Oct. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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26. AAV-CAG-jGCaMP8f-WPRE (AAV9)

    Viral Prep

    #179254-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from AAV-CAG-jGCaMP8f-WPRE (#179254). In addition to the viral particles, you will also receive purified AAV-CAG-jGCaMP8f-WPRE plasmid DNA. CAG-driven expression of calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger

    Promoter

    CAG

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

27. TFORF2408

    Plasmid

    #143370

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FOXF1 (FOXF1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. GFP-PH-FAPP1

    Plasmid

    #161986

    Purpose

    Expresses GFP-tagged PH-FAPP1. PI(4)P biosensor

    Depositor

    David Bryant, Tamas Balla

    Article

    Balla et al Mol Biol Cell. 2005 Mar;16(3):1282-95. d

    Insert

    PH-FAPP1 (PLEKHA3 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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29. pCMV-BEACON2

    Plasmid

    #171698

    Purpose

    Expresses BEACON2 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Cell Rep. 2020 Jun 2;31(9):107723. doi:

    Insert

    BEACON2
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    hAPOBEC3A_W98Y/W104A/Y130F

    Promoter

    CMV

    Available Since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCMV-dCas13-inactive M3nls

    Plasmid

    #157854

    Purpose

    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
    

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A; METTL3 D395A

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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