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Plasmid#118347PurposeThis plasmid expresses RNAi resistance version of hHSF1 to use for rescue experiments with HSF1 shRNA vector (plasmid #118345).DepositorInserthHSF1 (HSF1 Human)
UseTagsmyc and 6xHISExpressionMammalianMutationRNAi resistance mutations (does not change amino …PromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPB_TRE3G_scFV-sfGFP-Dot1LCD
Plasmid#235582PurposeDox-inducible expression of the catalytic-domain (CD) of epigenetic effector Dot1l fused with scFV to programme H3K79me2DepositorInsertDot1l (Dot1l Mouse)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB_TRE3G_scFV-sfGFP-Kmt5cFL
Plasmid#235581PurposeDox-inducible expression of the catalytic-domain (CD) of epigenetic effector Kmt5c fused with scFV to programme H4K20me3DepositorInsertKmt5c (Kmt5c Mouse)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB_TRE3G_scFV-sfGFP-Dnmt3a3lCD
Plasmid#235576PurposeDox-inducible expression of the catalytic-domain (CD) of epigenetic effector Dnmt3a3L fused with scFV to programme DNA methylationDepositorUseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB_TRE3G_scFV-sfGFP-Ring1bCD
Plasmid#235574PurposeDox-inducible expression of the catalytic-domain (CD) of epigenetic effector Ring1b fused with scFV to programme H2AK119ub.DepositorInsertRing1b (Rnf2 Mouse)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1
Plasmid#233078PurposeExpression vector for sgRNA-S1b and eCas12f1DepositorInsertU6-sgRNA-S1b (BsaI)-CBh-bpNLS-eCas12f1-2xFLAG-P2A-EGFP
UseCRISPRTags2xFLAGExpressionMammalianMutationPromoterhU6, CBhAvailable sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1420
Plasmid#228316PurposeHomoserine lactone (HSL)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterHomoserine lactone (HSL)-regulatable synthetic pr…Available sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT950
Plasmid#228290Purpose2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoter2,4-Diacetylphloroglucinol-regulatable synthetic …Available sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT957
Plasmid#228293PurposeConstitutive mutant PhlTA expression with strong promoterDepositorInsertphlTA mutant
UseSynthetic BiologyTagsThree copies of VP16 (VP48)-Nuclear localization …ExpressionYeastMutationE41GPromoterScPGK1 promoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1035
Plasmid#228300PurposeConstitutive expression of LuxTA mutant with strong promoterDepositorInsertluxTA mutant
UseSynthetic BiologyTagsNuclear localization signal-Three copies of VP16 …ExpressionYeastMutationS116Y, W201RPromoterKpGAPDH promoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT899
Plasmid#228288PurposeConstitutive mutant rPhlTA expression with strong promoterDepositorInsertrphlTA mutant
UseSynthetic BiologyTagsThree copies of VP16 (VP48)-Nuclear localization …ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143KPromoterKpGAPDH promoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-U6-gRNA scaffold-UbC-mCherry-PuroR
Plasmid#232183PurposeLentiviral plasmid for cloning an spCas9 gRNA with puromycin resistance and mCherryDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterU6, hUbCAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
p160Tol2-her4.3:switchCas9
Plasmid#227680PurposeExpression of cre inducible and her4.3 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs for SABER-seq in zebrafishDepositorInsertsloxP-dsRed-loxP-Cas9-t2A-GFP
4xU6:sgRNA
UseCRISPRTagsExpressionMutationPromoterU6 and her4.3Available sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-mSTING
Plasmid#229739PurposeExpresses mouse STING in mammalian cells. Stable expression with piggybac transposase.DepositorInsertSTING (Sting1 Mouse)
UseTagsNoneExpressionMammalianMutationPromoterEF1AAvailable sinceFeb. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) EGFP-Rac1(P29S)
Plasmid#224280PurposeCell transfection and expression of Rac1 (P29S)DepositorInsertRac1 (RAC1 Human)
UseTagsExpressionMammalianMutationP29SPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
RNF130 shRNA-TRE
Plasmid#225336PurposeshRNAmir backbone under tet-operator for RNA interference, with YFPDepositorInsertRNF130 shRNA (Rnf130 Mouse)
UseAAV and RNAiTagsExpressionMammalianMutationPromoterTRE (tetracycline-responsive element)Available sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-ArgiNLS-AausFP1
Plasmid#220602PurposeCre-dependent expression of a single-cell discriminating version of AausFP1 fluorescent protein.DepositorInsertAausFP1
UseAAV and Cre/LoxTagsArgiNLSExpressionMammalianMutationPromoterCAGAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
scQers promoter control #5 (high: EEF1A1 promoter, p028)
Pooled Library#217424PurposescQers promoter control #5 (high: EEF1A1 promoter, p028)DepositorExpressionBacterial and MammalianUseRNAiAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS59
Plasmid#215680PurposeCas9 + guide plasmid targeting ChrI split hygromycinR landing padDepositorInserteef-1A.1p::Cas9 + U6p::GTTTGAGTAGAGCACTCAGG
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGS101_EGFP(A206K)-CENP-T(1-242) AAVS donor
Plasmid#211867PurposeCENP-T(1-242) transgene integration at AAVS1 safe harbor locusDepositorInsertCENP-T(1-242) (CENPT Synthetic, Human)
UseCRISPRTagsExpressionMutationN/APromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL1_108
Plasmid#210692PurposeLevel 1 plasmid, barcoded ORIDepositorInsertpIM13 with barcode 28
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL1_107
Plasmid#210691PurposeLevel 1 plasmid, barcoded ORIDepositorInsertpCD6 ORI with barcode 27
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL1_106
Plasmid#210690PurposeLevel 1 plasmid, barcoded ORIDepositorInsertpCB102 ORI with barcode 26
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_ERBB2_PPP1R1B
Plasmid#205813PurposeExpress mEGFP-tagged fusion protein, ERBB2_PPP1R1B from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBP-C_NanoLuc
Plasmid#204112PurposeType 0 CDS partDepositorInsertNanoLuc luciferase
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW-LIC-N8C_Fab4a
Plasmid#203690PurposeExpresses N8C_Fab4aDepositorInsertN8C_Fab4a
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL1_37
Plasmid#199044PurposeLevel 1 plasmid, barcoded ORIDepositorInsertRSF1010 ORI plus NGS barcode 14
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL1_4
Plasmid#199036PurposeLevel 1 plasmid, barcoded ORIDepositorInsertpIP501 ORI plus NGS barcode 4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0001
Plasmid#141469PurposeLentiviral vector for overexpressing the HIF3A transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHIF3A (HIF3A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
AiP5001 RabV-CVS-N2cdeltaG-histone-EGFP
Plasmid#176283PurposeRecombinant rabies virus expressing histone-EGFPDepositorInsertH2B (H2BC11 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only