We narrowed to 7,945 results for: lif
-
Plasmid#134245PurposeRetroviral plasmid with H4 EGFP insertDepositorAvailable SinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only
-
pGL3 T2P 800
Plasmid#131147PurposeTo investigate activity of a 800 bp region upstream of mouse Trib2.DepositorInsert-800 - 0 of mTrib2 upstream of gene transcriptional start site (Trib2 Mouse)
TagsluciferaseExpressionMammalianAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3 T2P 2.6
Plasmid#131151PurposeTo investigate activity of a 2.6 kb region upstream of mouse Trib2.DepositorInsert-2.6kb - 0 of mTrib2 upstream of gene transcriptional start site (Trib2 Mouse)
TagsluciferaseExpressionMammalianAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFP-RIP2_3K
Plasmid#131205Purposemammalian expression, tet RDepositorInsertRIPK2 K209/410/538R (RIPK2 Human)
TagsEGFPExpressionMammalianMutationK209/410/538RPromoterpCMVAvailable SinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3 T2P 500
Plasmid#131150PurposeTo investigate activity of a 500 bp region upstream of mouse Trib2.DepositorInsert-500 - 0 of mTrib2 upstream of gene transcriptional start site (Trib2 Mouse)
TagsluciferaseExpressionMammalianAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_CtCHAD-H253A-R256A-R260A
Plasmid#127788PurposeExpresses a CtCHAD mutant protein in E. coli cellsDepositorInsertCtCHAD (CT0884 Chlorobium tepidum)
Tags6xHis tag + TEV cleavage siteExpressionBacterialMutationResidues 208-522 (CHAD domain), mutations in His2…Available SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_CtCHAD-H253A-R256A-R260A-R296A
Plasmid#127789PurposeExpresses a CtCHAD mutant protein in E. coli cellsDepositorInsertCtCHAD (CT0884 Chlorobium tepidum)
Tags6xHis tag + TEV cleavage siteExpressionBacterialMutationResidues 208-522 (CHAD domain), mutations in His2…Available SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_CtCHAD-R385A-K386A-K389A-K390A
Plasmid#127792PurposeExpresses a CtCHAD mutant protein in E. coli cellsDepositorInsertCtCHAD (CT0884 Chlorobium tepidum)
Tags6xHis tag + TEV cleavage siteExpressionBacterialMutationResidues 208-522 (CHAD domain), mutations in Arg3…Available SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBMN mCherry-NBR1(F562A/Y731A/ΔUBD)
Plasmid#119692PurposeFor mammalian expression of LIR double mutant NBR1 without its UBDDepositorInsertNBR1 (NBR1 Human)
UseRetroviralTagsmCherryExpressionMammalianMutationF562A/Y731A & UBD deletion (1-906 ), See depo…Available SinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBMN iRFP670-OPTN(F178A/D474N)
Plasmid#119694PurposeFor mammalian expression of iRFP-tagged LIR/UBD double mutant OPTNDepositorAvailable SinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pUC19_4xT790_28bp
Plasmid#114720Purpose4 x 28 bp repeats of the EGFR WT allele used as SiMREPS analyte standardDepositorInsertEGFR (EGFR Human)
ExpressionBacterialAvailable SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19_T790_28bp
Plasmid#114722Purpose28 bp fragment of the WT EGFR allele used as SiMREPS analyte standardDepositorInsertEGFR (EGFR Human)
ExpressionBacterialAvailable SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
PAK4-3'UTR-BS2
Plasmid#113091PurposeLuciferase reporter containing PAK4 3' UTR with the second predicted MiR-199a-3p bindingDepositorInsertp21 Activated kinase 4 (PAK4 Human)
UseLuciferaseExpressionMammalianMutationnonePromoterPGKAvailable SinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
PAK4-3'UTR-BS1
Plasmid#113090PurposeLuciferase reporter containing PAK4 3' UTR with the first predicted MiR-199a-3p binding siteDepositorInsertp21 Activated kinase 4 (PAK4 Human)
UseLuciferaseExpressionMammalianMutationnonePromoterPGKAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
NET39 Short pmRFP-N2 (451)
Plasmid#61981Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET39 (PPAPDC3 Human)
TagsmRFPExpressionMammalianMutationShort form of NET39PromoterCMVAvailable SinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-PIM2mut3
Plasmid#53742Purposepromoter region of PIM2 lacking the 2 forkhead binding sitesDepositorInsertPIM2 (PIM2 Human)
UseLuciferase; Reporter vectorMutationdeletion of 2 forkhead binding sites (bp# 395-497…Available SinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA_MET_D14_3Flag
Plasmid#182495PurposeContains cDNA of the MET gene without the exon 14DepositorAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-RpHLuorin2
Plasmid#171720PurposeLysosomal expression of ratiometric pHLuorin2DepositorInsertRatiometric pHLuorin2 fused to LAMP1 (LAMP1 Human)
Tagsratiometric pHluorin2ExpressionMammalianPromoterCMVAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.cTNT.iCre
Plasmid#69916PurposeUsed to package AAV9:cTNT.iCre, which specifically transduce cardiomyocytes and express Cre recombinase. Only works in vivo.DepositorHas ServiceAAV9Insertimproved cre (iCre) recombinase and tdTomato
UseAAVTagsThe iCre coding frame and tdTomato coding frame i…PromoterChicken cardiac troponin TAvailable SinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSPIH6
Plasmid#190676PurposeE. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)DepositorInsertsSmall ubiquitin-like modifier
Mxe GyrA intein with C-terminal chitin binding domain and His tag
TagsChitin binding domain, His tag, and N-terminal Hi…ExpressionBacterialMutationAdded C-terminal his tag and NonePromoterT7Available SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-neo-CD2-FAK
Plasmid#37013DepositorAvailable SinceOct. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-BCR-ABL-p190-FLAG
Plasmid#205620PurposeExpression of BCR-ABL oncogene in mammalian cellsDepositorInsertBCR-ABL oncogene, p190 isoform b3a2
TagsFLAGExpressionMammalianMutationT117M- Please see depositor commentsPromoterCMVAvailable SinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-TDP-43-A315T
Plasmid#133755PurposeLentiviral expression of human TDP-43 A315T disease mutant in mammalian cells.DepositorInsertTARDBP (TARDBP Human)
UseLentiviralExpressionMammalianMutationChanged alanine 315 to threoninePromoterCMVAvailable SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(H66)
Plasmid#63558PurposeExpression of the Azure (blue) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(H66)
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianMutationChanged tyrosine at position 66 with respect to t…PromoterCMV-EF1α hybrid (CEF)Available SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-TDP-43-M337V
Plasmid#133759PurposeLentiviral expression of human TDP-43 M337V disease mutant in mammalian cells.DepositorInsertTDP-43 (TARDBP Human)
UseLentiviralExpressionMammalianMutationChanged methionine 337 to valinePromoterCMVAvailable SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVXCMV100-mNeonGreen-PBD
Plasmid#241366PurposeLentiviral expression of mNeonGreen-tagged PAK1 p21-binding domain (PBD)DepositorInsertPak1 fragment (PAK1 Human)
UseLentiviralTagsmNeonGreenMutationaa 57-141PromoterTruncated CMV promoterAvailable SinceAug. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBABE HA Chk2 WT
Plasmid#41901DepositorAvailable SinceAug. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot8-D40AE42A_U
Plasmid#147647PurposeMammalian Expression of HsNot8-D40AE42ADepositorInsertHsNot8-D40AE42A (CNOT8 Human)
ExpressionMammalianAvailable SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
p(HA)HIF1alpha (P402A,P564A)
Plasmid#52636Purposemammalian expression of HA tagged HIF1alpha (full length) with P402A,P564A mutationDepositorInsertHIF1alpha P402A, P564A (HIF1A Human)
TagsHAExpressionMammalianMutationP402A, P564APromoterCMVAvailable SinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-TDP-43-Q331K
Plasmid#133761PurposeLentiviral expression of human TDP-43 Q331K disease mutant in mammalian cells.DepositorInsertTDP-43 (TARDBP Human)
UseLentiviralExpressionMammalianMutationChanged glutamine 331 to lysine.PromoterCMVAvailable SinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-foldon-GFP- PCP
Plasmid#194503PurposeExpression foldon-GFP-PCP protein for imaging of nonrepetitive DNADepositorInsertfoldon-GFP-PCP
UseLentiviralExpressionMammalianPromoterEF-1α core promoterAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
OTR untagged
Plasmid#208913Purposeencodes the human oxytocin receptor, without any tagsDepositorAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4.26-Ccnd2PromoterRegion1(1.2kb)
Plasmid#228058Purpose1.2 kb from mouse Ccnd2 promoter in front of firefly luciferase reporterDepositorInsertCcnd2 promoter (Ccnd2 Mouse)
UseLuciferaseAvailable SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-HBA-RIBEYE-eGFP
Plasmid#241988PurposeHuman RIBEYE cDNA cloned inframe into a pAAV-GFP vector driven by hybrid CMV-enhancer-human-beta-actin promoter (CMV-HBA) and containing downstream WPRE, Ampicillin ResistanceDepositorArticleInsertRIBEYE-GFP (CTBP2 Human)
UseAAVTagseGFPExpressionMammalianPromoterhybrid CMV-enhancer-human-beta-actin promoter (CM…Available SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/MPL-HA
Plasmid#204530PurposeMammalian expression of human MPL-HADepositorInsertMPL-HA (MPL Human)
ExpressionMammalianAvailable SinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
MYC-hTRAK2
Plasmid#225142PurposeExpresses MYC-tagged human TRAK2DepositorAvailable SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
NowGFP /pQE-30
Plasmid#74749PurposeNowGFP gene for bacterial expressionDepositorInsertNowGFP
TagsHisTag (with a mutation but still working)ExpressionBacterialMutationWasCFP with substitutions E6K, L42M, T43S, V68M, …PromoterT5Available SinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/CALR wt-FLAG
Plasmid#204532PurposeMammalian expression of human CALR wt-FLAGDepositorInsertCALR wt-FLAG (CALR Human)
ExpressionMammalianAvailable SinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPBbleo-ErbB2
Plasmid#197361PurposeEncoding human ErbB2.DepositorInsertERBB2 (ERBB2 Human)
ExpressionMammalianAvailable SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only