We narrowed to 24,337 results for: Spr

1. NAX_CAGGS_CAS9_Blasto

   Plasmid

   #167856

   Purpose

   PiggyBac compatible plasmid expressing spCas9

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Spacer Switch Plasmid

   Plasmid

   #215346

   Purpose

   Facilitates the change in spacer sequence to Neisseria gonorrhoeae encoding the Nla CRISPRi system.

   Depositor

   Hank Seifert

   Article

   Geslewitz et al mBio. 2023 Dec 21:e0302523. doi: 10.1128

   Insert

   OpaD spacer; Chloramphenicol Resistance Cassette
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGuide

   Plasmid

   #64711

   Purpose

   Cloning and expression of Streptococcus pyogenes guide RNA

   Depositor

   Kiran Musunuru

   Article

   Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

   Insert

   Streptococcus pyogenes guide RNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBlu/gRNA

   Plasmid

   #59188

   Purpose

   Serves as a shuttle vector for target oligo before insertion into Cas9 destination vector

   Depositor

   Robert Stupar

   Article

   Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

   Insert

   Guide RNA Cassette
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   U6 (Arabidopis)

   Available since

   Feb. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWalium20-10XUAS-3XFLAG-dCas9-VPR

   Plasmid

   #78897

   Purpose

   Expresses 3XFLAG-dCas9-VPR (human codon) under 10XUAS control, for in vivo CRISPRa

   Depositor

   Norbert Perrimon

   Article

   Lin et al Genetics. 2015 Oct;201(2):433-42. doi: 1

   Insert

   dCas9-VPR
   

   Use

   Tags

   3xFLAG

   Expression

   Insect

   Mutation

   Promoter

   10XUAS

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. sgTrack-mCherry

   Plasmid

   #114013

   Purpose

   Empty sgRNA expression vector with co-expression of mCherry reporter

   Depositor

   Charles Rudin

   Article

   Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-TO-nls-st1dCas9-3nls-3XGFP-2nls

   Plasmid

   #64113

   Purpose

   Expression of St1 dCas9-3XGFP in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   St1 dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3XsfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TO

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVDM1001

   Plasmid

   #134660

   Purpose

   CRISPR delivery and repair template delivery vector

   Depositor

   Willem van Schaik

   Article

   de Maat et al FEMS Microbiol Lett. 2019 Nov 1;366(22).

   Insert

   CRISPR guide RNA array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSQT834

   Plasmid

   #53371

   Purpose

   Csy4 and Cas9 nuclease expression plasmid

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   Csy4-T2A-Cas9-NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRU324

    Plasmid

    #167691

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::Cas9i
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC0044-EF1a-RanCas13b-NES-mapk

    Plasmid

    #103855

    Purpose

    Expresses RanCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    RanCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and mapk NES

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. sgTrack-GFP

    Plasmid

    #114012

    Purpose

    Empty sgRNA expression vector with co-expression of TurboGFP reporter

    Depositor

    Charles Rudin

    Article

    Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRU319

    Plasmid

    #167686

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYC2085

    Plasmid

    #158721

    Purpose

    CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using hygromycin as a selection marker.

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    Sth1 sgRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCFD2-dU6:2gRNA

    Plasmid

    #49409

    Purpose

    expression of gRNA under control of the Drosophila U6:2 promoter

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Insert

    dU6-2:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6-2

    Available since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPEY-GAL-Kan

    Plasmid

    #232099

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with KanMX selection

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPEY-GAL-Hyg

    Plasmid

    #232098

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with HygMX selection

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMLM3705

    Plasmid

    #47754

    Purpose

    Expresses mammalian cell codon-optimized dCas9-VP64

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    codon optimized dCas9-VP64
    

    Use

    CRISPR

    Tags

    3x FLAG, NLS, and VP64

    Expression

    Mammalian

    Mutation

    D10A and H840A mutations in Cas9 (catalytically i…

    Promoter

    CMV

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-SMVP-Cas9N-U6-gRNA M3

    Plasmid

    #80936

    Purpose

    Expresses Cas9N in mammalian cells; expresses gRNA M3 for Mstn cleavage.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. HP138-puro

    Plasmid

    #134246

    Purpose

    Cas9 in a transposon array under a Tet inducible promoter. For making inducible Cas9 cell lines. Puro selection.

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    Contains Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge