We narrowed to 7,375 results for: ef1a

1. pAAV-pEF1a-tTAc

   Plasmid

   #172123

   Purpose

   Constitutive expression of InteinC-tTAC

   Depositor

   Chun Xu

   Article

   Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

   Insert

   InteinC-tTAc
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCVL.SFFV.d14GFP.EF1a.HA.NLS.Sce(opt).T2A.TagBFP

   Plasmid

   #32627

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   Donor + Sce BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-R1R2_EF1ap300dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

   Plasmid

   #113343

   Purpose

   Expresses p300-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

   Depositor

   Gavin Wright

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   p300core-dCas9-VP64-T2A-MS2-p65-HSF1
   

   Use

   Piggybac

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKLV2-EF1a-Cas9GFP-W

   Plasmid

   #200100

   Purpose

   Lentiviral vector expressing Cas9 fused with GFP at the C-terminus

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1a-vCre

   Plasmid

   #55638

   Purpose

   EF1a-driven expression of VCre in Mammalian Cells

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde and AAV8

   Insert

   vCre
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available Since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-EF1aL-TagBFP-W

   Plasmid

   #126687

   Purpose

   Constitutive, ubiquitous blue fluorescence protein (BFP) expression.

   Depositor

   Darrell Kotton

   Article

   Jacob et al Nat Protoc. 2019 Dec;14(12):3303-3332. d

   Insert

   TagBFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Ef1aL

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. PEW-pEF1A-inactEGFP

   Plasmid

   #212009

   Purpose

   Cleavable backbone for constitutive expression of TCR alpha and beta chains through lentiviral transduction

   Depositor

   Catherine Wu

   Article

   Afeyan et al Nat Protoc. 2024 Nov 8. doi: 10.1038/s41

   Insert

   Inactivated eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pEF1a

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-EF1a-FLEX-GT

   Plasmid

   #26198

   Depositor

   Edward Callaway

   Article

   Wall et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

   Insert

   Enhanced green fluorescent protein, TVA (eGFP Mouse, TVA is a mouse codon optimized version of an avian gene.)
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mutation

   TVA mammalian codon optimized

   Promoter

   Available Since

   Sept. 16, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHIV-EF1a-2A-ZsGreen

   Plasmid

   #231985

   Purpose

   Empty backbone for expression of GoI with Bi-cistronic expression ZsGreen

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   2A-ZsGreen
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSicoR-Ef1a-mCh

    Plasmid

    #31847

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; Ef1alpha for mCherry

    Available Since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR_EF1a-->GssmCherry-PNE

    Plasmid

    #162157

    Purpose

    Lentiviral vector for constitutive expression of mCherry and PNE anchor in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2020 Oct 16;370(6514):327-331.

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1 EF1a-EGFP

    Plasmid

    #231925

    Purpose

    3rd generation lentiviral transfer plasmid with EGFP reporter gene

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-EF1a-V5-Bet1

    Plasmid

    #120243

    Purpose

    Expresses V5-tagged Bet1 in lentiviral vector

    Depositor

    Larry Gerace

    Insert

    Bet1 (Bet1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLQ5429_pUC_hU6-crScaffold_EF1a-BFP

    Plasmid

    #155306

    Purpose

    Rfx Cas13d guide cloning pUC19 vector with constitutively expressed tagBFP

    Depositor

    Stanley Qi

    Article

    Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

    Insert

    Rfx Cas13d CRISPR RNA BFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, EF1a

    Available Since

    July 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEF1a-hMLH1dn (og codon)

    Plasmid

    #174823

    Purpose

    Mammalian expression of human MLH1dn (original codon usage)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    human MLH1dn (original codon usage) (MLH1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deletion of residues 754-756

    Promoter

    EF1a

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE2-EF1aL-dsRed-W

    Plasmid

    #195863

    Purpose

    lentiviral expression of the red fluorescent protein driven by human EF1aL promoter

    Depositor

    Darrell Kotton

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    dsRed

    Expression

    Mutation

    Promoter

    EF1aL

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKLV-EF1aGFP2AKAT7(E508Q)-W

    Plasmid

    #159293

    Purpose

    Lentiviral vector expressing GFP and KAT7 (gRNA-resistant, E508Q mutant) driven by human EF1a promoter

    Depositor

    Kosuke Yusa

    Article

    Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

    Insert

    KAT7 E508Q
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    CRISPR sites mutated (gRNA ID. 5 and A10) and E50…

    Promoter

    human EF1a promoter

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE2-EF1aL-mCherry-W

    Plasmid

    #126703

    Purpose

    Constitutive, ubiquitous mCherry expression.

    Depositor

    Darrell Kotton

    Article

    McCauley et al Cell Stem Cell. 2017 Jun 1;20(6):844-857

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Ef1aL

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-EF1a-LIC-V5

    Plasmid

    #120248

    Purpose

    For LIC cloning with C-terminal V5 tag

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVH21-Tier1-PhEF1a

    Plasmid

    #169561

    Purpose

    Tier-1 PhEF1a-driven expression vector (PhEF1a-MCS-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pPB-R1R2_EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #112924

    Purpose

    piggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant marker

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
    

    Use

    Piggybac transposon vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHL-EF1a-SphcCas9-iP-A

    Plasmid

    #60599

    Purpose

    Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

    Depositor

    Akitsu Hotta

    Article

    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

    Insert

    CRISPR Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Codon usage optimized for human usage

    Promoter

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEF1a-Usp9x_CD-mCherry

    Plasmid

    #170380

    Purpose

    Vector for mammalian expression of the Usp9x catalytic domain tagged with mCherry, in pEF1a-IRES-Neo backbone.

    Depositor

    Miguel Ramalho-Santos

    Article

    Macrae et al Nat Commun. 2021 Mar 25;12(1):1865. doi:

    Insert

    Ubiquitin specific protease 9x catalytic domain (Usp9x Mouse)
    

    Use

    Tags

    mCherry-3xFlag

    Expression

    Mammalian

    Mutation

    Catalytic domain

    Promoter

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pT3-EF1A-CCNE2

    Plasmid

    #162554

    Purpose

    Overexpression of CCNE2

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    Overexpression of CCNE2 (CCNE2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    EF1A

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHAGE-EF1alphaL-hAAT-W

    Plasmid

    #24527

    Purpose

    Lentiviral overexpression

    Depositor

    Darrell Kotton

    Article

    Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

    Insert

    alpha-1 antitrypsin (SERPINA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. mc2 mNeon EF1a

    Plasmid

    #206224

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pKLV-EF1a-mCherryCXCR4-W

    Plasmid

    #200102

    Purpose

    Lentiviral vector expressing mCherry fused with CXCR4 cDNA at the C-terminus

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    CXCR4 (CXCR4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    silent mutations (c.180C>T, 438G>A, 495C>…

    Promoter

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLV-EF1a-NucGFP

    Plasmid

    #184706

    Purpose

    Lentiviral transfer plasmid for expression of NucGFP

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    NucGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-EF1a-sCre

    Plasmid

    #55635

    Purpose

    Expresses sCre in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHR_EF1a-->sGFP_PGK-->puroR

    Plasmid

    #162156

    Purpose

    Lentiviral vector for constitutive expression of GFP in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2020 Oct 16;370(6514):327-331.

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge