We narrowed to 3,111 results for: GAI
-
Plasmid#183925Purposep300 HAT core domain coupled to catalytically dead dCas9 for localization; labeled with GFPDepositorInsertdCas9-GFP-p300
UseCRISPRTagsNLSExpressionMammalianMutationnonePromoterCMVAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVUHshp53
Plasmid#11653DepositorInsertGFP, shRNA against TP53 (TP53 Human)
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSFFV-dNme2Cas9
Plasmid#193190PurposeLentiviral vector expressing nuclease-dead Cas9 from Neisseria meningitis (dNme2)DepositorInsertdNme2Cas9
UseTagsNLS and NLS-3xHA-NLSExpressionMammalianMutationH588A, D16APromoterSFFVAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pME-nls-sfGFP
Plasmid#132973Purposefull-length of nuclear localized sfGFP with stop codon in pmE Gateway vectorDepositorInsertnuclear localized super folder GFP
UseGateway vectorTags6x myc and SV40 NLSExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TetON-Puro-HIF1a 0819
Plasmid#118704PurposeLentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)DepositorInsertshRNA against human HIF1a
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX302 OAS2-V5 puro
Plasmid#158642PurposeLentiviral expression vector for constitutive expression of V5-tagged OAS2.DepositorInsertOAS2 (OAS2 Human)
UseLentiviralTagsV5ExpressionMutationPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TetON-Puro-HIF1a 3809
Plasmid#118705PurposeLentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)DepositorInsertshRNA against human HIF1a
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXSE901BG
Plasmid#91714PurposeCRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta and glyphosate-resistanceDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/FLAG/HA-DEST TNRC6B
Plasmid#19884DepositorInserttrinucleotide repeat containing 6B (TNRC6B Human)
UseTagsFLAG/HAExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
CMV-G-GECO1.0
Plasmid#32447PurposeMammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.0)DepositorInsertG-GECO1.0
UseTagsExpressionMammalianMutationGCaMP3 K119I/L173Q/S404G/E430VPromoterAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D-GAT3-HA-mCherry
Plasmid#117722PurposeExpresses a GABA transporter GAT-3 (Slc6a11)DepositorInsertSlc6a11 (Slc6a11 Mouse)
UseAAVTagsHA and mCherryExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
Nme2Cas9_pCDest
Plasmid#119923PurposeNme2Cas9 CMV-driven mammalian expression plasmidDepositorInsertNme2Cas9
UseTagsNLS-3xHA-NLSExpressionMammalianMutationPromoterCMVAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVk7-33G1-Flag
Plasmid#58950Purposebackbone for mouse recombinant antibody with added C-terminal FLAG tagDepositorTypeEmpty backboneUseTagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV-G-GECO1.1
Plasmid#32445PurposeMammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.1)DepositorInsertG-GECO1.1
UseTagsExpressionMammalianMutationGCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…PromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCNL-N1_vinculin
Plasmid#65691PurposeExpresses Vinculin-CNL in mammalian cellsDepositorInsertvinculin (Vcl Mouse)
UseLuciferaseTagsCNLExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVk7-33G1-BioLHis
Plasmid#58949Purposebackbone for mouse recombinant antibody with added C-terminal biotinylation site, linker, 6His tagDepositorTypeEmpty backboneUseTags6His tag and biotinylation peptideExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGF-mSox2-ONL
Plasmid#65719PurposemSox2 reporter plasmid (expresses ONL)DepositorInsertmurine Sox2 promoter and ONL
UseLentiviral and LuciferaseTagsExpressionMutationPromoterAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-6011nes
Plasmid#108652PurposeAn ERK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInserthyBRET-ERK-EV
UseTagsXFPExpressionMammalianMutationPromoterpCAGGSAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbsr-6013x
Plasmid#108654PurposeAn HRas biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInsertRaichu-EV
UseLentiviralTagsXFPExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBSE901
Plasmid#91709PurposeCRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta resistanceDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
NLS-HA-tdMCP-tdTomato
Plasmid#183938Purposetandem MS2 binding protein labeled with tdTomato, containing a NLS and a HA-tagDepositorInserttdMCP-tdTomato (cp Synthetic)
UseTagsNLS-HAExpressionMammalianMutationnonePromoterCMV (TATA box removed)Available sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGLTR-X-PURO
Plasmid#58246PurposeGATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and Puromycin selectionDepositorInsertPuro(R)
UseLentiviral and RNAi; Gateway destionation vectorTagsTetR-NLS-T2AExpressionMammalianMutationPromoterSFFVAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFRT/FLAG/HA-DEST EIF2C3
Plasmid#19889DepositorInsertEukaryotic translation initiation factor 2C, 3 (AGO3 Human)
UseTagsFLAG/HAExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-6020nes
Plasmid#108656PurposeA JNK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInserthyBRET-JNK-EV
UseTagsXFPExpressionMammalianMutationPromoterAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
rTetR-GFP-VP16
Plasmid#183917PurposeVP16 activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline additionDepositorInsertrTetR-GFP-VP16 (UL48 Synthetic)
UseTagsExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-sg-mGreenLantern/EGFP
Plasmid#179913PurposeAll in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.DepositorInsertmGreenLantern/EGFP sgRNA
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationPromoterU6Available sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSb_init containing the TM287/288 nanobody Nb_TM#2
Plasmid#132698Purposeexpresses the TM287/288 nanobody Nb_TM#2 in E.coli, to use as positive control in ELISADepositorInsertnanobody Nb_TM#2
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-G-GECO1.2
Plasmid#32468DepositorInsertG-GECO1.2
UseLab constructedTags6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…ExpressionBacterialMutationGCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…PromoterAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-IP2.0
Plasmid#109217PurposeExpress IP2.0 in E. coliDepositorInsertIP2.0 (Inverse Pericam 2.0)
UseTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX puro TRIM21 C54Y - GFP
Plasmid#116942PurposeExpression of TRIM21 C54Y -GFP fusionDepositorInsertTRIM21 C54Y-GFP (TRIM21 Human)
UseLentiviralTagsGFPExpressionMammalianMutationC54YPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-DIO-SN
Plasmid#181741PurposeAAV vector for expressing SNDepositorInsertSuperNova (monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation)
UseAAVTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBABE Zeo IkB SR
Plasmid#83023PurposeRetroviral expression of dominant negative IkB alphaDepositorInsertNFKB Inhibitor Alpha (NFKBIA Human)
UseRetroviralTagsExpressionMutationSerine 32 to Alanine, Serine 36 to AlaninePromoterAvailable sinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRZ-mNanog-YNL
Plasmid#65717PurposemNanog reporter plasmid (expresses YNL)DepositorInsertmurine Nanog promoter and YNL
UseLentiviral and LuciferaseTagsExpressionMutationPromoterAvailable sinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbsr-6085x
Plasmid#108653PurposeA tyrosine kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInsertPicchuEV
UseLentiviralTagsXFPExpressionMammalianMutationPromoterAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-6017nes
Plasmid#108655PurposeA PKA biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInserthyBRET-PKA-EV
UseTagsXFPExpressionMammalianMutationPromoterpCAGGSAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-6015nes
Plasmid#108657PurposeA S6 Kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInserthyBRET-S6K-EV
UseTagsXFPExpressionMammalianMutationPromoterpCAGGSAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO-TtAgocatdead
Plasmid#210322PurposeBacterial expression of the His- and SUMO-tagged catalytically inactive Argonaute protein of Thermus thermophilusDepositorInsertTtAgo (TT_P0026 Thermus thermophilus)
UseTags6x His tag and SUMO tagExpressionBacterialMutationPromoterT7Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYNL-N1_TUBB5
Plasmid#65678PurposeExpresses beta-tubulin-YNL in mammalian cellsDepositorInsertbeta-tubulin class V
UseLuciferaseTagsYNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGLTR-FP-GFP
Plasmid#58242PurposeGATEWAY-compatible lentiviral RNAi delivery vector; SFFV-driven eGFP markerDepositorInsertattR1-Cm(R)-CcdB-attR2
UseLentiviral and RNAi; Gateway destionation vectorTagsExpressionMammalianMutationPromoternoAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-B-GECO1
Plasmid#32469DepositorInsertB-GECO1.0
UseLab constructedTags6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…ExpressionBacterialMutationGCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…PromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pX459-sg-tdTomato90/816
Plasmid#179918PurposeDouble cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 90 and 816.DepositorInserttdTomato sgRNA
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationPromoterU6Available sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBXNH3LCA_TM287/288
Plasmid#132701Purposeexpresses TM287/288 in E.coli, to use as positive control in ELISADepositorUseTagsAvi and HisExpressionBacterialMutationPromoterpBADAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Myc-Foxo1-WT 6KQ
Plasmid#17559DepositorInsertFoxo1 6KQ (Foxo1 Mouse)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero9
Plasmid#187927PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero9DepositorInsertHero9
UseTagsFlag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
tdEos-Tubulin-C-18
Plasmid#57683PurposeLocalization: Microtubules, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertTubulin (TUBA1B Human)
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA-GFP-BIRC3-SU
Plasmid#160699PurposeExpresses GFP-BIRC3 with the short 3'UTR in mammalian cellsDepositorInsertGFP-BIRC3 (BIRC3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Teton-puro-shFAK #1
Plasmid#198757Purposeconditional knockdown of FAKDepositorInsertshFAK #1 (PTK2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Teton-puro-shFAK #2
Plasmid#198758Purposeconditional knockdown of FAKDepositorInsertshFAK #2 (PTK2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJBL704
Plasmid#140374PurposeExpresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 4BP dowsntream from the promoterDepositorInsertT7-4BP-tetO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBZ191-pU6-sgBFP-CBh-sv40NLS-Cas9-NLS-T2A-mCherry
Plasmid#170183PurposeExpression vector for a sgRNA against BFP and spCas9 linked to mCherry via a T2A peptideDepositorInsertsgBFP
UseTagsExpressionMammalianMutationPromoterhU6Available sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only