We narrowed to 54,957 results for: Ran;

1. pORANGE GFP-Syt7 KI

   Plasmid

   #131488

   Purpose

   Endogenous tagging of Synaptotagmin-7: N-terminal (amino acid position: P5)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Syt7, Syt7 Mouse, Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHIV-BRAFV600E-mOrange2

   Plasmid

   #110732

   Purpose

   Mammalian Expression of BRAFV600E IRES H2B-mOrange2

   Depositor

   Robert Judson-Torres

   Article

   Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

   Insert

   BRAFV600E IRES H2B-mOrange2 (BRAF Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   BRAF V600E

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pORANGE Cplx1-GFP KI

   Plasmid

   #131475

   Purpose

   Endogenous tagging of Complexin1: C-terminal (amino acid position: L128)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Cplx1, Cplx1 Mouse, Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLD1 translation factors

   Plasmid

   #117760

   Purpose

   BioBrick encoding cistrons 1-13 in pET for preparation of PURE 3.0 translation system from E. coli

   Depositor

   Anthony Forster

   Article

   Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

   Insert

   Translation factor cistrons 1-13
   

   Use

   Escherichia coli

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLD3 translation factors

   Plasmid

   #117762

   Purpose

   BioBrick encoding cistrons 22-30 in pET for preparation of PURE 3.0 translation system from E. coli

   Depositor

   Anthony Forster

   Article

   Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

   Insert

   Translation factor cistrons 22-30
   

   Use

   Escherichia coli

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLD2 translation factors

   Plasmid

   #117761

   Purpose

   BioBrick encoding cistrons 14-21 in pET for preparation of PURE 3.0 translation system from E. coli

   Depositor

   Anthony Forster

   Article

   Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

   Insert

   Translation factor cistrons 14-21
   

   Use

   Escherichia coli

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pORANGE GFP-CADPS KI

   Plasmid

   #131482

   Purpose

   Endogenous tagging of CAPS1: N-terminal (amino acid position: S39)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Cadps Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-Ran(Q69L)

   Plasmid

   #42048

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

   Insert

   Ran (RAN Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Q69L

   Promoter

   Available since

   Feb. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pORANGE GFP-Actb KI

   Plasmid

   #131479

   Purpose

   Endogenous tagging of βactin: N-terminal (amino acid position: D2)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Actb, Actb Mouse, Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiCrispr-V2-mOrange (V2mO)

    Plasmid

    #140206

    Purpose

    Lenti Crispr/Cas9 with mOrange fluorescent marker

    Depositor

    Jaffer Ajani

    Article

    Jin et al PLoS One. 2020 Feb 13;15(2):e0228910. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pELF1a

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pORANGE Gria1-GFP KI

    Plasmid

    #131489

    Purpose

    Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Gria1, Gria1 Mouse, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEMHE-membrane-mEGFP

    Plasmid

    #105526

    Purpose

    T7 promotor drives in vitro mRNA transcription of a mEGFP-tagged membrane reporter

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    GNAI2 (GNAI2 Human)
    

    Use

    In vitro transcription

    Tags

    mEGFP

    Expression

    Mutation

    fragment encoding the plasma membrane targeting s…

    Promoter

    T7

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. RANLS-DEVD-BNES

    Plasmid

    #50840

    Purpose

    Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddRFP A and ddRFP B
    

    Use

    Tags

    NES sequence LALKLAGLDIGS and triplicated NLS seq…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS-membrane-ceruleanFP

    Plasmid

    #53749

    Purpose

    Contains membrane localized cerulean fluorescent protein in pCS expression vector.

    Depositor

    Sean Megason

    Article

    Megason et al Methods Mol Biol. 2009;546:317-32. doi:

    Insert

    2x membrane linker
    

    Use

    Tags

    Cerulean

    Expression

    Mammalian

    Mutation

    Promoter

    simian CMV IE94

    Available since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pORANGE unc13a GFP KI

    Plasmid

    #131498

    Purpose

    Endogenous tagging of Munc13-1: C-terminal (amino acid position: A1762)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Unc13a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSTAR-mOrange2-sfGFP

    Plasmid

    #115693

    Purpose

    Generation of CD-tagged libraries with an mOrange2-sfGFP Mammalian Cell-Optimized Fluorescent Timer

    Depositor

    David Suter

    Article

    Alber et al Mol Cell. 2018 Aug 17. pii: S1097-2765(1

    Insert

    SA-mOrange2-sfGFP-SD
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL3-RANGAP.C-linker.miniIAA7.3xFlag.P2A.BSD

    Plasmid

    #216248

    Purpose

    HDR template to tag endogenous human RANGAP1 C-terminus with miniIAA7.3xFlag.P2A.BSD

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    HDR template for human RANGAP1 (RANGAP1 Human)
    

    Use

    CRISPR and TALEN; Hdr template

    Tags

    miniIAA7.3xFlag.P2A.BSD

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+)_Granulin 4-no linker

    Plasmid

    #176925

    Purpose

    express Granulin 4 without linker 5 in mammalian cells

    Depositor

    Thomas Kukar

    Article

    Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

    Insert

    Granulin 4 (GRN Human)
    

    Use

    Tags

    Twin-Strep and FLAG tags encoded after Signal Pep…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pORANGE GFP-Actb KI #2

    Plasmid

    #139666

    Purpose

    Endogenous tagging of β-actin: N-terminal (amino acid position: before startcodon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pORANGE Gria2-GFP KI

    Plasmid

    #131490

    Purpose

    Endogenous tagging of GluA2: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Gria2, Gria2 Mouse, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge