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176,067 results

Showing: 4261 - 4290 of 176067 results

  6. Mouse sgRNA library Brie in lentiGuide-Puro (Lentiviral Prep)

    Viral Prep
    #73633-LV
    Purpose
    Ready-to-use Lentiviral Prep particles produced from Mouse sgRNA library Brie in lentiGuide-Puro (#73633). In addition to the viral particles, you will also receive purified Mouse sgRNA library Brie in lentiGuide-Puro plasmid DNA. <p>&lt;p&gt;Ready-to-use lentiviral pooled library for CRISPR screening in mouse cells. Use on cells that are stably expressing Cas9 to make edits across 19,674 genes in the mouse genome.&lt;/p&gt;</p>
    Depositor
    John Doench, David Root
    Article
    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103
    Available Since
    Aug. 16, 2016
    Availability
    Academic Institutions and Nonprofits only

  7. pcDNA.3.1.ADRB2-NP

    Plasmid
    #134376
    Purpose
    Nanoluc complementation assay. Expression of adrenoceptor beta 2 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of signal sequence and Flag epitope at N terminus of ADRB2.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    ADRB2-NP (ADRB2 Human)
    Tags
    Flag, natural peptide of nanoluciferase, and sign…
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available Since
    Aug. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134376-plasmid-map-sequence-id-306396

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  8. pAAV.hSyn.eGFP.WPRE.bGH

    Plasmid
    #105539
    Purpose
    AAV expression of EGFP from hSyn promoter
    Depositor
    James M. Wilson
    Has Service
    AAV1
    Insert
    EGFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    March 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    105539-plasmid-map-sequence-id-457689

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  9. EasyCloneYALI- Integrative Vector Set

    Plasmid Kit
    #1000000140
    Purpose
    Integrative plasmids for overexpression of heterologous genes in Yarrowia lipolytica.
    Depositor
    Irina Borodina
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector Type
    Yeast Expression
    Cloning Type
    USER
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only

  10. pCDH-EF1-Luc2-P2A-copGFP

    Plasmid
    #72485
    Purpose
    Express Luc2 and copGFP
    Depositor
    Kazuhiro Oka
    Insert
    Luc2-P2A-copGFP
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1
    Available Since
    Jan. 11, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72485-plasmid-map-sequence-id-132335

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  11. pT22-GluCNZ-6His

    Plasmid
    #213504
    Purpose
    Designed for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.
    Depositor
    John Denu
    Article
    Kuznetsov et al J Biol Chem. 2018 Aug 3;293(31):12271-12
    Insert
    Glucanase (glucan endo-1,3-beta-glucosidase)
    Tags
    6His tag
    Expression
    Bacterial
    Mutation
    1-36 Signal Sequence Deletion
    Promoter
    T7
    Available Since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    213504-plasmid-map-sequence-id-424932

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  15. pSLQ10844

    Plasmid
    #183956
    Purpose
    CAG-hyperdCas12a-HA-miniVPR-mCherry-T2A-HygroR
    Depositor
    Stanley Qi
    Article
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Insert
    HyperdCas12a
    Use
    CRISPR
    Tags
    HA, Hygromycin Resistance, and mCherry
    Expression
    Mammalian
    Mutation
    D832A, D156R, E292R, D235R, D350R
    Promoter
    CAG
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    183956-plasmid-map-sequence-id-363081

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  17. pZac2.1 gfaABC1D-lck-GCaMP6f (AAV5)

    Viral Prep
    #52924-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pZac2.1 gfaABC1D-lck-GCaMP6f (#52924). In addition to the viral particles, you will also receive purified pZac2.1 gfaABC1D-lck-GCaMP6f plasmid DNA. gfaABC1D-driven (similar to GFAP promoter) lck-GCaMP6f calcium sensor expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Baljit Khakh
    Available Since
    April 19, 2018
    Availability
    Academic Institutions and Nonprofits only

  18. pSupAR-MbPylRS(DiZPK)

    Plasmid
    #91705
    Purpose
    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest
    Depositor
    P. Chen
    Article
    Zhang et al Nat Chem Biol. 2011 Sep 4;7(10):671-7. d
    Inserts
    pylS
    pylT
    Expression
    Bacterial
    Mutation
    L274A, C313S
    Available Since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    91705-plasmid-map-sequence-id-183278

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  22. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

    Plasmid
    #213026
    Purpose
    Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213026-plasmid-map-sequence-id-422069

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  23. Fragmid

    Plasmid Kit
    #1000000237
    Purpose
    Toolkit for rapid creation of CRISPR vectors compatible with various delivery approaches, enzymes, and mechanisms.
    Depositor
    John Doench
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    AAV, Insect Expression, Lentiviral, Mammalian Expression
    Editing Type
    CRISPR
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 4261 - 4290 of 176067 results