We narrowed to 25,364 results for: Nes
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Plasmid#222330PurposeFluorescent reporter plasmid for VeillonellaDepositorInsertbs2 gene with Veillonella parvula SKV38 mdh promoter
UseE. coli-veillonella shuttle plasmidExpressionBacterialPromoterVeillonella parvula SKV38 mdh promoterAvailable SinceOct. 31, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA-dCas9
Plasmid#47106PurposeExpresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cellsDepositorInsertdCas9
UseCRISPRTagsFlag and SV40 NLSExpressionMammalianMutationD10A, H840A (catalytically inactive)PromoterCMVAvailable SinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUBQ:Citrine-TurboID-3xMyc
Plasmid#199244PurposeThis plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.DepositorInsertCitrine-TurboID-3xMyc
TagsTurboID-3xMycExpressionPlantPromoterArabidopsis UBQ10 promoter with intronAvailable SinceJune 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
2xP53_RE::NLuc
Plasmid#124533Purposep53 NLuc luciferase reporterDepositorInsertP53 NLuc reporter
UseLuciferase and Synthetic BiologyExpressionMammalianAvailable SinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
YE1-BE4max-NG
Plasmid#138159PurposeC-to-T base editorDepositorInsertrAPOBEC1(YE1)-nSpCas9(NG)-UGI-UGI
TagsNLSExpressionMammalianMutationWithin rAPOBEC1 W90Y + R126E; within Cas9 D10A + …PromoterCMVAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant
Plasmid#101209PurposeBacterial expression plasmid for SpCas9-HF1 variantDepositorInsertSpCas9 variant N497A/R661A/Q695A/Q926A
Tags10x His, MBP, and TEV siteExpressionBacterialMutationN497A, R661A, Q695A and Q926APromoterT7Available SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBABE FLAG-IKK2-EE puro
Plasmid#216790PurposeMammalian expression of constitutively active human IKK-2DepositorInsertIKK-2 (IKBKB Human)
UseRetroviralTagsFLAGExpressionMammalianMutationS177E and S181E, constitutively activePromoterLTRAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pB-MultiplexedReporter-MOR170-1
Plasmid#129461PurposepiggyBac transposon vector encoding the receptor MOR170-1 and the barcoded CRE reporter geneDepositorInsertMOR170-1 (Olfr895 Mouse)
UseSynthetic BiologyTagsLUCY tag, Rho tagExpressionMammalianPromoterTRE (Tet-On)Available SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pV1393
Plasmid#111430PurposeCaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)DepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRExpressionYeastAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDGB3alpha2_35S:P19:Tnos (GB1203)
Plasmid#68214PurposeTranscriptional unit for Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression driven by the 35S promoterDepositorInsertP19 silencing suppressor
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable SinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEF-BOS-RIG-I-Myc
Plasmid#215872PurposeRIG-I-Myc expression vector for overexpressionDepositorInsertRIG-I (RIGI Human)
ExpressionMammalianAvailable SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
TNPO1-shRNA
Plasmid#86082PurposeA lentiviral construct for building stable cell lines expressing Transportin-1 shRNA via puromycin selection.DepositorAvailable SinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xHA-MEK1-DD
Plasmid#47576PurposeBacterial expression plasmid for GST-3xHA-tagged constitutively active MEK1DepositorInsertMEK1-DD (Map2k1 Mouse)
Tags3xHA and GSTExpressionBacterialMutationSer218 to Asp, Ser221 to AspAvailable SinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAV-Cre-GFP
Plasmid#68544PurposeAAV expression of Cre-GFP fusionDepositorInsertCre
UseAAV and Cre/LoxTagsEGFPExpressionMammalianPromoterCMVAvailable SinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEasyG3_nat
Plasmid#184918PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG3_mic
Plasmid#184920PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
p(A)n-PAUSE
Plasmid#118068PurposeTranscription Blocker - Synthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin geneDepositorInsertSynthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin gene
UseSynthetic BiologyExpressionMammalianAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
hCMV-IE1:Renilla
Plasmid#118066PurposeTranscriptional unit for constitutive expression of Renilla in mammalian cellsDepositorInsertCMV:GreenRenilla:bGHF
UseLuciferase and Synthetic Biology; Assembly of gb2…ExpressionMammalianPromoterCMVAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK172-6xHis-0N4R-P301S-tau-HiBiT
Plasmid#187835PurposeExpression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.DepositorInsert6XHis-0N4R-P301S-tau-HiBiT
Tags6xHistidine and HiBiTExpressionBacterialPromoterT7Available SinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xFlag-JNK1a1
Plasmid#47574PurposeBacterial expression plasmid for GST-3xFlag-tagged JNK1a1DepositorAvailable SinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xHA-MKK6-EE
Plasmid#47579PurposeBacterial expression plasmid for GST-3xHA-tagged constitutively active MKK6DepositorInsertMKK6-EE (MAP2K6 Human)
Tags3xHA and GSTExpressionBacterialMutationSer207 to Glu, Thr211 to GluAvailable SinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLX302-MKP5-V5 puro
Plasmid#87770PurposeExpresses V5 tagged human MAPK phosphatase 5 (MKP5/DUSP10)DepositorAvailable SinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
NEK7_HUMAN_D0
Plasmid#79735PurposeThis plasmid encodes the kinase domain of NEK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRedF
Plasmid#118057PurposeGBPart - RedF Luciferase CDSDepositorInsertRed Firefly Luciferase CDS
UseSynthetic Biology; Domestication of dna parts for…MutationRed mutant S286YAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAEL010097-Cas9
Plasmid#100707PurposeThe promoter of AAEL010097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL010097-hspcas9-GFP
Opie2-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectPromoterAAEL010097 and Opie2Available SinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_WiChR_TS_mScarlet_ER
Plasmid#205997PurposeHigh efficient, K+ selective channelrhodopsin for the inhibition of neuronsDepositorHas ServiceAAV9InsertWobblia Inhibitory Channelrhodopsin
UseAAVTagsTS, mScarlet, ERPromoterhSynAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO shGATA6 puro
Plasmid#72615PurposeExpresses an inducible short hairpin targeting human GATA6 sequenceDepositorInsertshort hairpin GATA binding protein 6
UseLentiviralTagsNoneExpressionMammalianPromoterTRE TightAvailable SinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
5xNF-kb_RE::RedF
Plasmid#124530PurposeNFKB RedFirefly luciferase reporterDepositorInsertNF-κβ RedF reporter
UseLuciferase and Synthetic BiologyExpressionMammalianAvailable SinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-MFSD8-Puro
Plasmid#239954PurposeLentiviral vector to generate MFSD8 stable expressing cell line under CMV promoterDepositorInsertMFSD8
UseLentiviralTagsmyc-flagExpressionMammalianAvailable SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-His:dCas9:nls:10P3
Plasmid#135982PurposeExpresses dCas9 fused to 10 copies of the P3 coiled coil in mammalian cellsDepositorInsertdCas9 fused to 10 copies of the P3 coiled-coil
UseCRISPRTagsHisExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only