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pGEx-4T-1-YTHDF3

Plasmid

#70088

Purpose

Encode YTHDF3 isoform a with N-terminal GST tag for E. coli expression

Depositor

Chuan He

Article

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTH N(6)-methyladenosine RNA binding protein 3 (YTHDF3 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only
pYLCRISPR/Cas9P35S-N

Plasmid

#66191

Purpose

expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), Neo selection

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Insert

Cas9

Expression

Plant

Mutation

plant-codon optimized with higher GC contents (62…

Promoter

cauliflower mosaic virus 35S promoter (P35S)

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG_mRuby2_smFP FLAG

Plasmid

#59760

Purpose

encodes multiple copies of an epitope tag FLAG ( not detected by anti GFP ab)

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

mRuby2_smFP_FLAG

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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mRIP3 GFP wt

Plasmid

#41382

Depositor

Francis Chan

Article

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP3 (Ripk3 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only

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pCMX-Gal4-TEAD4

Plasmid

#33105

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

Insert

TEAD4 (TEAD4 Human)

Tags

Gal4

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pBF3060

Plasmid

#26853

Purpose

Expression of yeast codon-optimized CreA recombinase from the GAL1 promoter

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Insert

CreA recombinase

Use

Cre/Lox

Expression

Yeast

Mutation

N/A

Promoter

GAL1

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-NTRK3

Plasmid

#23901

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NTRK3 (NTRK3 Human)

Use

Gateway Cloning

Mutation

F441L and L653P relative to NM_001012338.3

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-RET

Plasmid

#23906

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RET (RET Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag HK2

Plasmid

#20501

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

HK2 (HK2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pMSCV-FlagMLL2-pL-ENL(5613)

Plasmid

#20874

Depositor

Robert Slany

Article

Bach et al Oncogene. 2009 Feb 12. 28(6):815-23.

Insert

MLL2 (Kmt2d, MLLT1 Mouse, Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

April 21, 2009

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag Jnk1a2

Plasmid

#13751

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk1a2 (MAPK8 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pGEX-4T1-14-3-3 epsilon GST

Plasmid

#13279

Depositor

Michael Yaffe

Article

Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

Insert

14-3-3 epsilon (Ywhae Rat)

Tags

GST

Expression

Bacterial

Available Since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

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pGEX-XIAP

Plasmid

#8340

Depositor

Jon Ashwell

Article

Yang et al Science 2000 May 5;288(5467):874-7.

Insert

XIAP (XIAP Human)

Tags

GST

Expression

Bacterial

Available Since

March 14, 2006

Availability

Academic Institutions and Nonprofits only

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pSFFV_mNG3A(1-10)

Plasmid

#157992

Purpose

Lentiviral vector of constitutive expression of mNeonGreen3A(1-10)

Depositor

Bo Huang

Article

Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

Insert

pSFFV_mNG3K(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

W148L, M150V, K153M, N194K, F204L

Promoter

SFFV

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-FLPo

Plasmid

#118027

Purpose

AAV vector for sparse and bright labeling in a cell-type specific manner

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

Insert

TRE-DIO-FLPo

Use

AAV

Promoter

TRE

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pGWB454

Plasmid

#74836

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with mRFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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1119 pBabe puroL HA FKHR AAA

Plasmid

#9025

Depositor

William Sellers

Article

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

FKHR AAA (FOXO1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

T24A S256A S319A

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
GFP-mROCK2

Plasmid

#101296

Purpose

Mammalian expression of Rock2-GFP

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock2 (Rock2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody

Plasmid

#199390

Purpose

Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tag

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

anti-ALFA nanobody

Tags

14xHis-Avi-SUMOEu

Expression

Bacterial

Promoter

T5-LacO

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pGD-p19

Plasmid

#196326

Purpose

35S promoter-driven expression of the tomato bushy stunt virus p19 RNA silencing suppressor

Depositor

Zhenghe Li

Article

Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:

Insert

tomato bushy stunt virus p19

Expression

Plant

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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