We narrowed to 24,076 results for: cas9 genes

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Tol2_B-act_dCas9-Vp64-T2A-Citrine

Plasmid

#200248

Purpose

Zebrafish transgenics; Plasmid encodes dCas9-Vp64 and could be stably integrated in the zebrafish genome using Tol2 transgenesis

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

dCas9-Vp64-T2A-Citrine

Use

Zebrafish expression

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGG>nls-hCas9-nls-GFP

Plasmid

#99141

Purpose

Cas9-GFP fusion protein under the regulation of chicken beta-actin promoter

Depositor

Marianne Bronner

Article

Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

Insert

Cas9-GFP

Use

CRISPR

Tags

GFP

Promoter

Chicken beta actin (CAG)

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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Eef1a-1955/-1>nls::Cas9::nls

Plasmid

#59987

Purpose

Eef1a (EF1alpha) promoter driving nls::Cas9::nls

Depositor

Lionel Christiaen

Article

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Insert

nls::Cas9::nls

Use

CRISPR

Mutation

Reverted mutations from dCas9 to wiltdtype

Promoter

Ciinte.Eef1a (EF1alpha) -1955/-1

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-pCALM1-SpCas9-miniU6-sgRNAShank3

Plasmid

#213973

Purpose

AAV vector to express SpCas9 driven by pCALM1 promoter for targeting Shank3 locus

Depositor

Zhonghua Lu

Article

Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

Insert

Shank3 sgRNA

Use

AAV and CRISPR

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-LTR-dCas9-VP64-BFP

Plasmid

#46912

Purpose

Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFP

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Inserts

dCas9-VP64-BFP fusion
Puromycin resistance

Use

CRISPR and Retroviral

Tags

3xNLS, BFP, and VP64 domain

Expression

Mammalian

Promoter

LTR and PGK

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-dCas9-T2A-GFP

Plasmid

#53191

Purpose

Co-expresses human optimized S. pyogenes dCas9 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized dead Cas9 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D10A and H840A

Promoter

hUbC

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Dnmt3a-P2A-tagBFP

Plasmid

#84569

Purpose

Lentiviral construct to express dCas9 fused with Dnmt3a-P2A-tagBFP

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Dnmt3a (DNMT3A Synthetic, Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pT3TS-spCas9 ABE-Umax-ex1

Plasmid

#222141

Purpose

Adenine base editor with modified TadA variant for extended window for A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Promoter

T3

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-MMLVgag-D3A-3L-dCas9-KOX1

Plasmid

#240532

Purpose

Expresses MMLVgag–DNMT3A-3L-dCas9-KOX1 for producing RENDER-DNMT3A-3L-dCas9-KOX1

Depositor

James Nuñez

Article

Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

Insert

MMLVgag-D3A-3L-dCas9-KOX1

Tags

FLAG, HA

Expression

Mammalian

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pspCas9-ABE-3'UTR-sgRNA-2xMS2

Plasmid

#132554

Purpose

Vector plasmid expressing ABEmax and sgRNA scaffold with MS2 replacing the Tetraloop and loop 2

Depositor

Baisong Lu

Article

Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

Inserts

ABEmax
sgRNA, with Tetraloop and loop 2 replaced by MS2 aptamer

Expression

Mammalian

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-Anc689-UdgX-nCas9-RBMX

Plasmid

#163552

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-Anc689-UdgX-nCas9-RBMX

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9(H840A)-T2A-EGFP

Plasmid

#221231

Purpose

Express nCas9 nickase (H840A) with GFP reporter

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

SpCas9(H840A)-T2A-EGFP

Expression

Mammalian

Mutation

H840A

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-CFP-SaCas9-HF (without sgRNA)

Plasmid

#134470

Purpose

Human expression vector for SaCas9-HF variant.

Depositor

Zongli Zheng

Article

Tan et al Proc Natl Acad Sci U S A. 2019 Sep 30. p

Insert

Staphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity

Use

CRISPR

Tags

SV40NLS-3xFLAG

Expression

Mammalian

Mutation

R245A, N413A, N419A, R654A

Promoter

CAG

Available Since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgDrd2

Plasmid

#159918

Purpose

Mutagenesis of Drd2

Depositor

Larry Zweifel

Insert

Drd2 (Drd2 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pGMC00025 (aka pCW-dCas9-Blast)

Plasmid

#195316

Purpose

Dox inducible lentiviral dead Cas9

Depositor

Raj Chari

Insert

dead Cas9

Use

CRISPR and Lentiviral

Expression

Bacterial and Mammalian

Mutation

D10A; H840A

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-SpCas9-NG-P2A-EGFP (RTW3677)

Plasmid

#139994

Purpose

CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…

Promoter

CMV and T7

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-ACTA2_gRNA1-SpCas9-T2A-GFP

Plasmid

#126706

Purpose

to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequence

Depositor

Darrell Kotton

Article

Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

Insert

ACTA2_gRNA1-SpCas9-T2A-GF (ACTA2 Human)

Use

CRISPR

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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PET-HiFi SpCas9-NLS-6xHis

Plasmid

#207376

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

HiFi SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R691A

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pSpdCas9-huTET1CD-T2A-GFP (PX458)

Plasmid

#129025

Purpose

targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNA

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA cloning site

Tags

HA-Tag, NLS and T2A-EGFP

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-TRE3G-dCas9-EGFP-HaloTag

Plasmid

#169451

Purpose

expression of dCas9-EGFP-HaloTag

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

dCas9-EGFP-HaloTag

Use

Lentiviral

Available Since

June 13, 2021

Availability

Academic Institutions and Nonprofits only

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