We narrowed to 13,167 results for: Mpl;

1. pPPAT-2XStrep

   Plasmid

   #125620

   Purpose

   expresses human PPAT protein with a C-terminal 2XStrep affinity tag

   Depositor

   Stephen Benkovic

   Article

   Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

   Insert

   PPAT (PPAT Human)
   

   Use

   Tags

   2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAcrF2

   Plasmid

   #89234

   Purpose

   Plasmid contains the gene for anti-CRISPR protein AcrF2 (gene 30 from bacteriophage D3112), with N-terminal 6his tag and TEV protease cleavage site

   Depositor

   Blake Wiedenheft

   Article

   Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

   Insert

   AcrF2
   

   Use

   Tags

   6xHis-TEV

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA5 HA-Arp8

   Plasmid

   #19796

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

   Insert

   Arp8 (ACTR8 Human)
   

   Use

   Tags

   2XHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pBacPAK8-His-myc-Rbx1

   Plasmid

   #29506

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

   Insert

   Rbx1 (Rbx1 Mouse)
   

   Use

   Tags

   His and myc

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pCIneo-lambdaN-HA-HsSmg6_I

   Plasmid

   #146551

   Purpose

   Mammalian Expression of HsSmg6

   Depositor

   Elisa Izaurralde

   Article

   Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

   Insert

   HsSmg6 (SMG6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   two silent mutations T594C, A699G and two non sil…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GFP-BICD2N

   Plasmid

   #64070

   Purpose

   N-terminal 400 amino acids of mouse BICD2 codon optimized for Sf9 expression with a N-terminal GFP tag and a TEV-cleavable His-ZZ tag for purification (GFP-BICD2N)

   Depositor

   Andrew Carter

   Article

   Schlager et al EMBO J. 2014 Sep 1;33(17):1855-68. doi:

   Insert

   BICD2N (1-400) (Bicd2 Mouse)
   

   Use

   Tags

   GFP and His-ZZ-TCS

   Expression

   Insect

   Mutation

   Codon optimized for Sf9 expression

   Promoter

   Available since

   Sept. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. FLAG-MED6

   Plasmid

   #15411

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

   Insert

   MED6 (Med6 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pfastbacHTb/mClock26-384

   Plasmid

   #47336

   Purpose

   mClock fragment cloned into pfastbacHTb vector with Hexa His tag

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   Clock (Clock Mouse)
   

   Use

   Tags

   His and Tev

   Expression

   Mutation

   aa 26-384

   Promoter

   PH

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pKM296

   Plasmid

   #134180

   Purpose

   Mammalian expression of CENP-C and KNL2 CDK SA

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

   Insert

   mcherry-CENP-C 510-end-KNL2 CDK SA (CENPC Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX6P1 UBXN11

    Plasmid

    #169017

    Purpose

    Expresses GST-UBXN11 (human) in bacteria

    Depositor

    Hemmo Meyer

    Article

    Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

    Insert

    UBXN11 (UBXN11 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. KIF5C(1-560)-mCitrine

    Plasmid

    #61676

    Purpose

    Single mCitrine-labeled, truncated kinesin-1

    Depositor

    Kristen Verhey

    Article

    Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

    Insert

    KHC(1-560)-mCitrine (Kif5c Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. KIF5C(1-560)-GFP11

    Plasmid

    #61670

    Purpose

    Non-fluorescent, truncated kinesin-1 fused to split GFP linker

    Depositor

    Kristen Verhey

    Article

    Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

    Insert

    KIF5C(1-560)-GFP11 (Kif5c Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CFF-EGFP

    Plasmid

    #86061

    Purpose

    A mammalian expression plasmid encoding fibrocystin mimentic clone CFF (CD8a luminal domain, fibrocystin transmembrane and cytosolic domains) with C-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Fibrocystin (Pkhd1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains luminal and transmembrane domains of CD8…

    Promoter

    CMV

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCFJ2309 - [1-2] ENTR - Fluor - mCardinal(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

    Plasmid

    #159861

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - mCardinal(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LN-entry

    Plasmid

    #128012

    Purpose

    Vector containing the lambdaN-entry expression cassette and a separate expression cassette driving mCherry via the traffic jam enhancer

    Depositor

    Julius Brennecke

    Article

    Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

    Insert

    LN-3xFlag
    

    Use

    Lamdan entry vector

    Tags

    LN-3xFlag

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLENTI_RPS3_K227/230R

    Plasmid

    #127135

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    RPS3 (RPS3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    K227R/K230R

    Promoter

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLENTI_RPS3_K230R

    Plasmid

    #127134

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    RPS3 (RPS3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    K230R

    Promoter

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLENTI_RPS3_K227R

    Plasmid

    #127133

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    RPS3 (RPS3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    K227R

    Promoter

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pT7-V5-SBP-C1-Hs4EBP3_Z

    Plasmid

    #148151

    Purpose

    Mammalian Expression of Hs4EBP3

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

    Insert

    Hs4EBP3 (EIF4EBP3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCFJ2443 - [1-2] ENTR - Fluor - ce-mMaple3(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid

    #159867

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-mMaple3(PATCs(900bp), NLS, no_atg, no stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET19b-MED17

    Plasmid

    #15412

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

    Insert

    MED17 (MED17 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    Available since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

22. [pSK475] pLJC5 HA-Depdc5(A)

    Plasmid

    #109344

    Purpose

    Lenti-viral expression of HA-tagged Depdc5 mutant A

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    DIYGD(185-189)GSGSG

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. [pSK476] pLJC5 HA-Depdc5(B)

    Plasmid

    #109345

    Purpose

    Lenti-viral expression of HA-tagged Depdc5 mutant B

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    EQPLH(371-375)GSGSG

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. [pSK478] pLJC5 HA-Depdc5(P)

    Plasmid

    #109347

    Purpose

    Lenti-viral expression of HA-tagged Depdc5 mutant P

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    YDLLP(775-779)GSGSG

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. R4pGWB601_UBQ10p-BirA(R118G)-YFP-NLS

    Plasmid

    #127365

    Purpose

    Binary vector for expressing nuclear BirA* (R118G)-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    BirA* (R118G mutant)
    

    Use

    Tags

    GS linker, NLS, V5, and YFP

    Expression

    Plant

    Mutation

    R118G

    Promoter

    Arabidopsis UBQ10 promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGEMHE:mTREK-1(K271Q)

    Plasmid

    #133270

    Purpose

    X. laevis expression vector. It will generate the mouse TREK-1 channel

    Depositor

    Dan Minor

    Article

    Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

    Insert

    Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 )
    

    Use

    Oocyte expression

    Tags

    Expression

    Mutation

    K271Q

    Promoter

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TNPO1-shRNA

    Plasmid

    #86082

    Purpose

    A lentiviral construct for building stable cell lines expressing Transportin-1 shRNA via puromycin selection.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Transportin-1 (TNPO1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAcrF1

    Plasmid

    #89233

    Purpose

    Plasmid contains the gene for anti-CRISPR protein AcrF1 (gene 35 from bacteriophage JBD30), with N-terminal 6his tag and TEV protease cleavage site

    Depositor

    Blake Wiedenheft

    Article

    Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

    Insert

    AcrF1
    

    Use

    Tags

    6xHis-TEV and FLAG

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAC8_MF (VE5596)

    Plasmid

    #139768

    Purpose

    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    PH or p10

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLENTI_RPS3_K214R_Keima

    Plasmid

    #127143

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    RPS3_Keima (RPS3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    K214R

    Promoter

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLenti6.3/V5-DEST-PKCi-A120E-ΔPB1

    Plasmid

    #56658

    Purpose

    expresses constitutively active human PKCi with a deletion on the PB1 domain

    Depositor

    Pedro Salas

    Article

    Forteza et al Biol Open. 2013 Oct 8;2(11):1264-9. doi:

    Insert

    Human PKCi (PRKCI Human)
    

    Use

    Lentiviral

    Tags

    V5/His

    Expression

    Mutation

    A120E and deletion of PB1 domain (aa 24–98)

    Promoter

    CMV

    Available since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

32. pGM55

    Plasmid

    #71249

    Purpose

    luciferase reporter with hGM-CSF -55 to +28 minimal promoter

    Depositor

    Peter Cockerill

    Article

    Bert et al Plasmid. 2000 Sep;44(2):173-82.

    Insert

    GM-CSF minimal promoter (-55 to +28) (CSF2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    GM-CSF minimal

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pscALPS-SIV-AGM-TAN1 vpr

    Plasmid

    #115819

    Purpose

    Encodes codon optimized 3xFLAG-SIVAGM Vpr (TAN1) (U58991) with repaired premature stop codon and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVAGM Vpr (TAN1)
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEBTet-SNAP-HsSAS6

    Plasmid

    #136789

    Purpose

    Mammalian expression of the centrosomal protein SAS6 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-SAS6 (SASS6 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3-HA-REDD1 R133A/P139A (#626)

    Plasmid

    #99946

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    R133A/P139A

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pmcsg7-mCRT(deltaP)

    Plasmid

    #83507

    Purpose

    Bacterial expression of Calreticulin mutant

    Depositor

    Malini Raghavan

    Article

    Del Cid et al J Biol Chem. 2010 Feb 12;285(7):4520-35.

    Insert

    CALR (Calr Mouse)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    delta . Deletion of residues 187-283

    Promoter

    T7

    Available since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

37. pmcsg7-mCRT(deltaC)

    Plasmid

    #83506

    Purpose

    Bacterial expression of Calreticulin mutant

    Depositor

    Malini Raghavan

    Article

    Del Cid et al J Biol Chem. 2010 Feb 12;285(7):4520-35.

    Insert

    CALR (Calr Mouse)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    delta C. Contains residues 1-339

    Promoter

    T7

    Available since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

38. pcDNA1 3XFlag ATXN1[15Q]-delAXH

    Plasmid

    #33241

    Depositor

    Huda Zoghbi

    Article

    Lam et al Cell. 2006 Dec 29;127(7):1335-47.

    Insert

    ATXN1[2Q]-delAXH (ATXN1 Human)
    

    Use

    Tags

    3xflag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

39. pCIneo-V5-SBP-HsSmg6-del36-576-siRNAres_I

    Plasmid

    #146553

    Purpose

    Mammalian Expression of HsSmg6-del36-576-siRNAres

    Depositor

    Elisa Izaurralde

    Article

    Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

    Insert

    HsSmg6-del36-576-siRNAres (SMG6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. DYNLL1

    Plasmid

    #132530

    Purpose

    Human LC8-1, codon optimised for Sf9 expression.

    Depositor

    Andrew Carter

    Article

    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

    Insert

    DYNLL1 (DYNLL1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Codon optimised for Sf9 expression

    Promoter

    Available since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge