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Addgene

We narrowed to 9,532 results for: SUB

Showing: 7551 - 7600 of 9532 results
  1. AAV r3.45

    Plasmid
    #233684
    Purpose
    An evolved Adeno-associated viral variant, enhances gene delivery and gene targeting in neural stem cells
    Depositor
    Insert
    Modified AAV2 Rep, Cap
    Use
    AAV
    Tags
    Expression
    Mutation
    insertion of LATQVGQKTA (where LA and A are linke…
    Promoter
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
  2. pPicZ:mTREK-1_cryst_I110D

    Plasmid
    #209640
    Purpose
    P. pastoris expression vector. It will generate the mouse TREK-1 channel (21-322, I110D) fused to a C-terminal GFP
    Depositor
    Insert
    Potassium channel subfamily K member 2 (KCNK2, TREK-1) I110D mutant
    Use
    Tags
    SNS- 3C_cleavage_site-TAAA-GFP
    Expression
    Yeast
    Mutation
    aa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…
    Promoter
    Available since
    Sept. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. AA125

    Plasmid
    #215982
    Purpose
    Fragmid fragment: (N' terminus) activator from p65 subunit of mouse Nfkb
    Depositor
    Has Service
    Cloning Grade DNA
    Insert
    Start; p65_v1.3; HSF1_v1.2; Linker_v3.1; SV40NLS_v1.6
    Use
    Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. PCUP1-GFP-Osh1-406

    Plasmid
    #207007
    Purpose
    Integration of Osh1-GFP as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Yeast PMN pathway substrate.
    Depositor
    Insert
    OSH1
    Use
    Tags
    GFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pGG-GTS-TurboID-GFP

    Plasmid
    #209405
    Purpose
    Transiently expressing and visualizing the subcellular localization of TurboID fused with a Golgi targeting sequence (GTS) in planta
    Depositor
    Insert
    GTS-3XHA-TurboID
    Use
    Tags
    GFP
    Expression
    Plant
    Mutation
    TurboID gene sequence C249A
    Promoter
    Cauliflower mosaic virus (CaMV) 35S promoter
    Available since
    Jan. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pRH3175

    Plasmid
    #210177
    Purpose
    Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-R33G,G141S-GFP::tADH1
    Depositor
    Insert
    YIp ADE2-LEU2 pTDH3::aro7-R33G,G141S-GFP::tADH1 (ARO7 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    R33G,G141S
    Promoter
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. pHR-LS

    Plasmid
    #205438
    Purpose
    Expression of Gaussia luciferase (gLuc) and spectinomycin resistance (Spec) genes
    Depositor
    Inserts
    Gaussia luciferase gene
    spectinomycin resistance gene
    Use
    Cre/Lox and Luciferase; Chlamydomonas expression
    Tags
    Expression
    Mutation
    Promoter
    Heat shock protein 70A (HSP70A)/ribulose bisphosp…
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. pGBT9-GGA2-GAE

    Plasmid
    #199175
    Purpose
    Expression of GAL4 DNA-binding domain (BD)-GGA2-GAE domain fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Insert
    GGA2-GAE domain (GGA2 Human)
    Use
    Tags
    GAL4-DNA binding domain fragment
    Expression
    Yeast
    Mutation
    Contains Lys 545 Gln substitution
    Promoter
    ADH1
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
  9. iEscSnFR

    Plasmid
    #182807
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Insert
    iEscSnFR
    Use
    Tags
    6xHis, HA and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
  10. iFluoxSnFR

    Plasmid
    #182808
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Insert
    iFluoxSnFR
    Use
    Tags
    6xHis, HA and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
  11. pDD2604

    Plasmid
    #175088
    Purpose
    Mammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T604A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).
    Depositor
    Insert
    KSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S644A
    Promoter
    Available since
    Feb. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  12. pDD2602

    Plasmid
    #175086
    Purpose
    Mammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T424A, T438A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).
    Depositor
    Insert
    KSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    T424A, T438A
    Promoter
    Available since
    Feb. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  13. pDD2603

    Plasmid
    #175087
    Purpose
    Mammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T504A, T508A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).
    Depositor
    Insert
    KSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    T504A, T508A
    Promoter
    Available since
    Feb. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 7551 - 7600 of 9532 results