We narrowed to 15,924 results for: aga
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Plasmid#126698Purposeto express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human SCGB3A2 locus at the end of the endogenous coding sequenceDepositorInsertsgRNA targeting human SCGB3A2 locus
UseTagsExpressionMammalianMutationPromoterHuman U6Available sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBRY-nuclear mCherry-IRES-PURO
Plasmid#52409PurposeConstitutively expressed mammalian expression vector encoding nuclear mCherry fluorescent reporter. Puromycin resistance.DepositorInsertnuclear mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
ACE Reporter (pLenti-CMV-mCherry (+43) -T2A-eGFP)
Plasmid#109428PurposeA lentiviral backbone expressing mCherry with a 43 basepair insert and eGFP off of a CMV promoter. A reporter for both Cas9 nuclease and APOBEC-mediated base-editing activity.DepositorInsertmCherry T2A GFP
UseLentiviralTagsExpressionMutationInserted 43 base pairs into mCherry to create rep…PromoterCMVAvailable sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO-RFP-IKZF1-sh2
Plasmid#69041Purpose3rd generation lentiviral vector expressing IKZF1 shRNADepositorInsertIKZF1 (IKZF1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
hRAD18 dC2-EGFP
Plasmid#68826PurposeExpresses human RAD18 (deleting Polymerase eta binding domain) tagged with EGFPDepositorInserthuman RAD18 (deleting Polymerase eta binding domain) tagged with EGFP (RAD18 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
Flag-Jun4A-Myc
Plasmid#47444Purposemammalian expression of mutant c-Jun (four N-terminal residues phosphorylated by JNK are mutated into Alanines)DepositorInsertJun 4A mutant (Jun Mouse)
UseTagsFlag and mycExpressionMammalianMutationfour N-terminal residues phosphorylated by JNK, s…PromoterCMVAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
GFP KNL1 wt hardened and RVSF->AAAA
Plasmid#45225DepositorInsertGFP KNL1 with RVSF-AAAA mutation, resistant to KNL1 siRNA (KNL1 Human)
UseTagsExpressionMutationRVSF mutated to AAAAPromoterAvailable sinceMay 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-PKHD1-myc
Plasmid#108407PurposeCan be used to generate stable Flp-In cells with tetracycline-inducible human polyduction/fibrocystin expression.DepositorInsertPKHD1 (PKHD1 Human)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pORANGE Rims2-GFP KI
Plasmid#131495PurposeEndogenous tagging of RIM2: C-terminal (amino acid position: S1551)DepositorUseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHRIG-PRAS40
Plasmid#53599Purposelentiviral expression of constitutively active PRAS40 and EGFPDepositorInsertPRAS40 (AKT1S1 Human)
UseLentiviralTagsIRES-EGFP and mycExpressionMammalianMutationconstitutively active (myristoylated version)PromoterCMVAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-GFP-PKHD1
Plasmid#108408PurposeCan be used to generate stable Flp-In cells with tetracycline-inducible human polyduction/fibrocystin expression.DepositorInsertPKHD1 (PKHD1 Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pY036_ATP1A1_G5_Array
Plasmid#86620PurposeVector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G5 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmidDepositorInsertATP1A1 G5 crRNA + user-specified crRNA + AsCpf1-3xHA
UseCRISPR; Co-selection via hdr using ouabainTags3xHAExpressionMammalianMutationPromoterCBhAvailable sinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
Raichu-OsRac1 CA
Plasmid#133264PurposeConstitutively-active (CA) mutant of OsRac1; Acts as a positive control for small GTPases activation assays.DepositorInsertOsRac1(G19V) (LOC4325879 Oryza sativa)
UseTagsCFP-lipid and Venus-CRIBExpressionPlantMutationOsRac1 G19VPromoterUbiAvailable sinceJan. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
Raichu-OsRac1 DN
Plasmid#133263PurposeDominant-negative (DN) mutant of OsRac1; Acts as a negative control for small GTPases activation assaysDepositorInsertOsRac1(T24N) (LOC4325879 Oryza sativa)
UseTagsCFP-lipid and Venus-CRIBExpressionPlantMutationOsRac1 T24NPromoterUbiAvailable sinceJan. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV/myc/ER-αT2ib
Plasmid#110769PurposeExpresses ER intrabody against mouse and human TLR2DepositorInsertintrabody against mouse and human TLR2
UseTagsER retention signal (SEKDEL), ER signal peptide, …ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-HA-hCaspase 2 prodomain-VC155
Plasmid#49261PurposeExpresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.DepositorInsertcaspase-2 prodomain (CASP2 Human)
UseTagsHA and split Venus CExpressionMammalianMutationdelete aa 148-435PromoterCMVAvailable sinceDec. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBIFC-flag-hCaspase 2 prodomain-VN173
Plasmid#49262PurposeExpresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.DepositorInsertcaspase-2 prodomain (CASP2 Human)
UseTagsflag and split Venus NExpressionMammalianMutationdelete aa 148-435PromoterCMVAvailable sinceDec. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTBL680 CHYRON3 integration construct
Plasmid#126448PurposeTo integrate the CHYRON3 locus at AAVS1 in human cells.DepositorInsertspU6/3xLacO-CHYRON3 hgRNA
pCMV-puro
UseTagsExpressionMammalianMutationThe SpCas9 sgRNA constant region is mutated to ma…PromoterCMV and human U6/3xLacOAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
STARR-seq luciferase INF enhancer vector_ORI_OAS3
Plasmid#99324PurposeLuciferase validation vector with OAS3 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activityDepositorInsertenhancer; chr12: 94575894 -94578286
UseLuciferase; Starr-seq luciferase validation vectorTagsExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pbG-APRT-J-u32uni-PdTKmCh
Plasmid#107279PurposeAPRT c.407T>C (APRT*J) donor vector with unilateral microhomologyDepositorInsertsUseTagsExpressionMutationc.402A>T, c.407T>CPromoterAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBW2614_pCAG-PV1-GAI-L2-28-396-FlpO-ABI-NLS-BGHpA
Plasmid#108833PurposeExpresses aa28 to aa396 of Flp recombinase fused to GAI and ABI CIDsDepositorInsertFlp recombinase fragment aa28 to aa396
UseCre/Lox and Synthetic BiologyTagsABI,NLS and GAIExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-HA-hCaspase 2 CARD-VC155
Plasmid#49259PurposeExpresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.DepositorInsertCaspase-2 CARD (CASP2 Human)
UseTagsHA and split Venus CExpressionMammalianMutationdeleted aa 123-435PromoterCMVAvailable sinceDec. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-flag-hCaspase 2 CARD-VN173
Plasmid#49260PurposeExpresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.DepositorInsertcaspase-2 CARD (CASP2 Human)
UseTagsFlag and split Venus NExpressionMammalianMutationdelete aa 123-435PromoterCMVAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-flag-hCaspase 2 FL (C303S)-VN173
Plasmid#49258PurposeExpresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.DepositorInsertcaspase-2 (CASP2 Human)
UseTagsflag and split Venus NExpressionMammalianMutationC303SPromoterCMVAvailable sinceMay 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCCLc-U6-shHMGA2.3-PGK-dTomato
Plasmid#89606PurposeSilencing Hmga2 in human cellsDepositorInsertshRNA targeting HMGA2 (HMGA2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY036_ATP1A1_G3_Array
Plasmid#86619PurposeVector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G3 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmidDepositorInsertATP1A1 G3 crRNA + user-specified crRNA + AsCpf1-3xHA
UseCRISPR; Co-selection via nhej using ouabainTags3xHAExpressionMammalianMutationPromoterCBhAvailable sinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLT 652
Plasmid#59997PurposeExpresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progenyDepositorUseTagsExpressionBacterialMutationpartial genomicPromoterAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
STARR-seq luciferase validation vector_SCP1_SV40
Plasmid#99311PurposeLuciferase validation vector with SV40 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activityDepositorInsertSV40 enhancer
UseLuciferase; Starr-seq luciferase validation vectorTagsExpressionMammalianMutationPromoterAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
CDK3 gRNA (BRDN0001147261)
Plasmid#76765Purpose3rd generation lentiviral gRNA plasmid targeting human CDK3DepositorInsertCDK3 (CDK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcFLAG-CSK-delta SH3 (73 to450 amino acids)
Plasmid#74504PurposeOverexpression of CSK delta SH3 in mammalian cellsDepositorInsertCSK (CSK Human)
UseTagsFLAGExpressionMammalianMutationdel-SH3 (73 to450 amino acids)PromoterCMVAvailable sinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only