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20,441 results

Showing: 7901 - 7950 of 20441 results

  1. GST-mDia1(FH1FH2DAD)-His

    Plasmid
    #85822
    Purpose
    contains the formin homolgy domains: FH2 is required for nucleation, processive tracking of barbed-end of actin filaments ; FH1 permits enhancement of actin polymerization rate.
    Depositor
    Antoine Jégou, Guillaume Romet-Lemonne
    Article
    Jegou et al Nat Commun. 2013;4:1883. doi: 10.1038/nc
    Insert
    Protein diaphanous homolog 1 (Diaph1 Mouse)
    Use
    Tags
    8*His and GST
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85822-plasmid-map-sequence-id-183287

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  10. SpySwitch

    Plasmid
    #184225
    Purpose
    Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.
    Depositor
    Mark Howarth
    Article
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    SpySwitch
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    E77A mutation prevents isopeptide bond formation,…
    Promoter
    T7
    Available since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184225-plasmid-map-sequence-id-361604

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  14. pENN.AAV.CamKII.GCaMP6f.WPRE.SV40

    Plasmid
    #100834
    Purpose
    AAV expression of ultrasensitive protein calcium sensor from CamKII promoter
    Depositor
    James M. Wilson
    Has Service
    AAV1, AAV5, and AAV9
    Insert
    GCaMP6f
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G
    Promoter
    CamKII
    Available since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    100834-plasmid-map-sequence-id-259370

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  15. pENN.AAV.CAG.tdTomato.WPRE.SV40

    Plasmid
    #105554
    Purpose
    AAV expression of tdTomato from CAG promoter
    Depositor
    James M. Wilson
    Insert
    tdtomato
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105554-plasmid-map-sequence-id-202390

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  31. pAB1

    Plasmid
    #62547
    Purpose
    broad host range plasmid for IPTG-inducible expression of eGFP. Designed for imaging Pseudomonas aeruginosa activity in biofilms.
    Depositor
    Michael Franklin
    Article
    Walters MC et al Antimicrob Agents Chemother. 2003 Jan;47
    Insert
    eGFP
    Use
    Broad host range plasmid
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pTrc
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    62547-plasmid-map-sequence-id-314155

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  39. pTOX6

    Plasmid
    #127451
    Purpose
    Encodes Tse2 toxin and amilCP chromoprotein. CAM-R
    Depositor
    Matthew Waldor
    Article
    Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii
    Inserts
    tse2 toxin
    amilCP chromoprotein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Codon optimized from protein sequence in FPbase
    Promoter
    pRha and tac
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127451-plasmid-map-sequence-id-251155

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  41. pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090

    Plasmid
    #103871
    Purpose
    dPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to selectively edit adenosine to inosine in RNA when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Promoter
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    103871-plasmid-map-sequence-id-259670

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  44. DsRed2-C1

    Plasmid
    #54611
    Purpose
    Localization: C1 Cloning Vector, Excitation: 558, Emission: 583
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    DsRed2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54611-plasmid-map-sequence-id-257389

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  45. CMV-GFP-Kir2.1

    Plasmid
    #107184
    Purpose
    mammalian expression of Kir2.1
    Depositor
    Bikash Pattnaik
    Insert
    KCNJ2 (KCNJ2 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    107184-plasmid-map-sequence-id-203927

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  50. PARP1 FL

    Plasmid
    #169815
    Purpose
    Expresses codon optimised full length His tagged PARP1 in bacterial cells
    Depositor
    David Neuhaus
    Article
    Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226
    Insert
    Poly[ADP-ribose] polymerase 1 (PARP1 Codon optimised, Synthetic, Human)
    Use
    Tags
    MKHHHHHHMKQ
    Expression
    Bacterial
    Mutation
    Valine 762 mutated to an alanine
    Promoter
    T7 promoter
    Available since
    Aug. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    169815-plasmid-map-sequence-id-334563

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Showing: 7901 - 7950 of 20441 results