195,499 results

1. mCherry-WASH1-N-18

   Plasmid

   #55163

   Purpose

   Localization: Cytoskeleton, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   WASH1
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

2. mCherry-WASH1-N-18

   Plasmid

   #55163

   Purpose

   Localization: Cytoskeleton, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   WASH1
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pEAK10-His-Myc-Kal7

   Plasmid

   #25454

   Depositor

   Betty Eipper

   Article

   Penzes et al J Biol Chem. 2000 Mar 3. 275(9):6395-403

   Insert

   Kal7
   

   Tags

   His and Myc

   Expression

   Mammalian

   Available Since

   July 13, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEAK10-His-Myc-Kal7

   Plasmid

   #25454

   Depositor

   Betty Eipper

   Article

   Penzes et al J Biol Chem. 2000 Mar 3. 275(9):6395-403

   Insert

   Kal7
   

   Tags

   His and Myc

   Expression

   Mammalian

   Available Since

   July 13, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. lentiCRISPR v2-sgDHODH-4

   Plasmid

   #186022

   Purpose

   knock out DHODH in mammalian cells

   Depositor

   Boyi Gan

   Article

   Mao et al Nature. 2021 May;593(7860):586-590. doi:

   Insert

   Dihydroorotate Dehydrogenase (Quinone) (DHODH Human)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET30b-5M SrtA with TEV site

   Plasmid

   #86962

   Purpose

   Expresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tag

   Depositor

   Ron Bose

   Article

   Sarpong et al Anal Biochem. 2017 Mar 15;521:55-58. doi

   Insert

   S.aureus SrtA 5M
   

   Tags

   6x Histidine tag and TEV site

   Expression

   Bacterial

   Mutation

   P94R, D160N, D165A, K190E, K196T

   Promoter

   T7

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV2.1-CMV-BD lacZ_3' Cer-bGHpA

   Plasmid

   #216319

   Purpose

   Split fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).

   Depositor

   Elvir Becirovic

   Article

   Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

   Insert

   Split Cerulean + splice acceptor site
   

   Use

   AAV

   Promoter

   CMV

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV2.1-CMV-5'Cer-BDlacZ-bGHpA

   Plasmid

   #216318

   Purpose

   Split fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).

   Depositor

   Elvir Becirovic

   Article

   Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

   Insert

   split cerulean fluorescent protein + splice donor site
   

   Use

   AAV

   Promoter

   CMV

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pIA1414

   Plasmid

   #172402

   Purpose

   Expression of SARS-COV-2 Nsp9 in E. coli

   Depositor

   Irina Artsimovitch

   Article

   Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

   Insert

   SARS-COV-2 Nsp9 (ORF1ab Synthetic)
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Ubi:ccdb-eGFP + RUBY

    Plasmid

    #233310

    Purpose

    GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of eGFP paired with ccdb selection toxin and RUBY marker

    Depositor

    Thomas Baum

    Insert

    Ubi:ccdb-eGFP + RUBY
    

    Use

    Gateway destination

    Tags

    eGFP

    Available Since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. Ubi:ccdb + RUBY

    Plasmid

    #233306

    Purpose

    GATEWAY compatible vector with soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of ccdb selection toxin and RUBY marker

    Depositor

    Thomas Baum

    Insert

    Ubi:ccdb + RUBY
    

    Use

    Gateway destination

    Available Since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAV-U6+27-Tornado-F30-TAR Variant-1

    Plasmid

    #129406

    Purpose

    circular TAR Variant-1

    Depositor

    Samie Jaffrey

    Article

    Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

    Insert

    circular F30-TAR Variant-1
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. lentiCRISPR v2-sgDHODH-2

    Plasmid

    #186023

    Purpose

    knock out DHODH in mammalian cells

    Depositor

    Boyi Gan

    Article

    Mao et al Nature. 2021 May;593(7860):586-590. doi:

    Insert

    Dihydroorotate Dehydrogenase (Quinone) (DHODH Human)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV_GR-RE_MLPmin_BC1094-luc2

    Plasmid

    #227114

    Purpose

    Glucocorticoid receptor response element for luciferase and barcode assays; barcode BC1094

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    12x clustered GR response element linked to MLP minimal promoter driving barcode 1094 and a luciferase reporter gene
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXint129

    Plasmid

    #178208

    Purpose

    Encodes bacteriophage lamda excisionase and integrase to facilitate in vivo Gateway LR clonase reaction.

    Depositor

    Gregory Phillips

    Article

    Platt et al Plasmid. 2000 Jan . 43(1):12-23.

    Insert

    xis excisionase, int integrase (xis, lambdap32 Bacteriophage lambda)
    

    Expression

    Bacterial

    Promoter

    lacI

    Available Since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pN1-COX8-mScarletI

    Plasmid

    #129341

    Purpose

    Mitochondria matrix localization

    Depositor

    Yasushi Okada

    Insert

    mScarlet(i) (Addgene 85044)
    

    Expression

    Mammalian

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBabe zeo HMGA2

    Plasmid

    #17411

    Depositor

    Tyler Jacks

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

    Insert

    HMGA2 (Hmga2 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    March 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBabe zeo HMGA2

    Plasmid

    #17411

    Depositor

    Tyler Jacks

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

    Insert

    HMGA2 (Hmga2 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    March 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCEP4-myc-EFNB2 (WT)

    Plasmid

    #200975

    Purpose

    Mammalian expression plasmid for wild type, myc-tagged EFNB2

    Depositor

    Erik Procko

    Article

    Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

    Insert

    EFNB2 (EFNB2 Human)
    

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCW57.1_V5_APEX_FLAG_Ct

    Plasmid

    #194069

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    FLAG-APEX-V5

    Expression

    Mammalian

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCDNA3.1 Bach1 S990A

    Plasmid

    #73643

    Purpose

    Mammalian expression of Bach1 S990A mutant (BRCA binding mutant).

    Depositor

    Sharon Cantor

    Insert

    Bach1 (BACH1 Human)
    

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    S990A (BRCA binding mutant)

    Promoter

    CMV

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

22. pLB038 - PHS1∆P

    Plasmid

    #136285

    Purpose

    entry vector for GreenGate cloning method, C module, open reading frame of phosphatase-inactive PROPYZAMIDE-HYPERSENSITIVE 1 (PHS1) (PHS1DP)

    Depositor

    Alexis Maizel

    Article

    Vilches Barro et al Curr Biol. 2019 Aug 5;29(15):2443-2454.e

    Insert

    PHS1∆P
    

    Expression

    Bacterial

    Available Since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. pPOTv7-blast-3HA

    Plasmid

    #221048

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Samuel Dean

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    3xHA epitopes
    

    Use

    Unspecified

    Promoter

    read through

    Available Since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TFORF0943

    Plasmid

    #143246

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    LMO2 (LMO2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAF0185 Cp36_attP-mCherry donor

    Plasmid

    #193468

    Purpose

    Promoterless mcherry donor for Cp36

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Cp36_attP
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-TaqStoffel (JO595)

    Plasmid

    #208952

    Purpose

    A variant CE1 construct with Taq Stoffel DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-TaqStoffel-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. RXFP2-Tango

    Plasmid

    #66493

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    RXFP2 (RXFP2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mTORC1 Focused library

    Pooled Library

    #164084

    Purpose

    Lentiviral CRISPR library targets human mTORC1 signaling pathway

    Depositor

    David Sabatini

    Article

    Condon et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

29. GFP-PKI nls

    Plasmid

    #118301

    Purpose

    Expression of PKIa

    Depositor

    Philip Stork

    Article

    Billiard et al J Biol Chem. 2001 Aug 17;276(33):31238-4

    Insert

    PKI nls (aa 2-36)
    

    Tags

    Flag and GFP

    Expression

    Mammalian

    Mutation

    inserting 2xnls

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAF0286 Ef1a-Cp36_attB-BFP acceptor

    Plasmid

    #193474

    Purpose

    Acceptor plasmid for 3 plasmid recombination assay

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Cp36_attB
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pECE-M2-PP1Cbeta

    Plasmid

    #31677

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    protein phosphatase 1, catalytic subunit, beta isozyme (PPP1CB Human)
    

    Tags

    M2

    Expression

    Mammalian

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

32. pECE-M2-PP1Cbeta

    Plasmid

    #31677

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    protein phosphatase 1, catalytic subunit, beta isozyme (PPP1CB Human)
    

    Tags

    M2

    Expression

    Mammalian

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

33. pBiT3.1-C_PALB2:1-1186

    Plasmid

    #211160

    Purpose

    Mammalian expression of full-length human PALB2. C-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    PALB2:M1-S1186 (PALB2 Human)
    

    Tags

    GSSGGSSGVSGWRLFKKIS

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available Since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. RXFP4-Tango

    Plasmid

    #66495

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    RXFP4 (RXFP4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

35. pCS2 xenopus chordin myc

    Plasmid

    #24682

    Depositor

    Edward De Robertis

    Article

    Piccolo et al Cell. 1996 Aug 23. 86(4):589-98.

    Insert

    Chordin
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    June 7, 2010

    Availability

    Academic Institutions and Nonprofits only

36. pCS2 xenopus chordin myc

    Plasmid

    #24682

    Depositor

    Edward De Robertis

    Article

    Piccolo et al Cell. 1996 Aug 23. 86(4):589-98.

    Insert

    Chordin
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    June 7, 2010

    Availability

    Academic Institutions and Nonprofits only

37. pDONR221-SV2C_STOP

    Plasmid

    #161463

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SV2C (SV2C Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pGEX-4T-1 3xHA-MKK6-EE

    Plasmid

    #47579

    Purpose

    Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK6

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    MKK6-EE (MAP2K6 Human)
    

    Tags

    3xHA and GST

    Expression

    Bacterial

    Mutation

    Ser207 to Glu, Thr211 to Glu

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

39. pGEX-4T-1 3xHA-MKK6-EE

    Plasmid

    #47579

    Purpose

    Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK6

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    MKK6-EE (MAP2K6 Human)
    

    Tags

    3xHA and GST

    Expression

    Bacterial

    Mutation

    Ser207 to Glu, Thr211 to Glu

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

40. pCR1063

    Plasmid

    #111091

    Purpose

    Expresses dCas9-KRAB tagged with mCherry: pHR-hEF1a-dCas9-mcherry-KRAB-mPGK-Hyg

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    dCas9-KRAB
    

    Use

    Lentiviral

    Tags

    mCherry

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge