We narrowed to 7,308 results for: RAP

1. MTK3b_031

   Plasmid

   #123809

   Purpose

   Encodes Kruppel Associated Box repression domain fused to methyl CpG binding protein 2 as a Type 3b part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   KRAB:meCP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLN599 (pFuGW-hUbCp-hSTE1)

   Plasmid

   #105206

   Purpose

   ubiquitin C promoter drives STE expression

   Depositor

   Timothy Lu

   Article

   Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

   Insert

   hUbCp-hSTE1
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-ZnF/HIT1

   Plasmid

   #15332

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Cai et al J Biol Chem. 2005 Apr 8. 280(14):13665-7

   Insert

   zinc finger, HIT domain containing 1 (ZNHIT1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

4. px330 VRER

   Plasmid

   #101729

   Purpose

   Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VRER
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VRER (D1135V, G1218R, R1335E, and T1337R mutation…

   Promoter

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPI278

   Plasmid

   #113227

   Purpose

   extrachromosomal expression vector, expression of H2B-GFP

   Depositor

   Rob Kay

   Article

   Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

   Insert

   H2B
   

   Use

   Dictyostelium discoideum expression vector, extra…

   Tags

   GFP

   Expression

   Mutation

   Promoter

   act15

   Available since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMET7-GAG-HRAS

   Plasmid

   #80604

   Purpose

   Mammalian expression vector that expresses the HIV-1 GAG protein with HRAS fused to it

   Depositor

   Sven Eyckerman

   Article

   Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

   Insert

   HIV-1 p55 GAG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SRalpha

   Available since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJBL726

   Plasmid

   #140396

   Purpose

   Expresses SmtB-regulated 3WJdB RNA aptamer driven by T7 promoter with the smtO sequence 5BP downstream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-smtO-3WJdB-T
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMZ-440

   Plasmid

   #195464

   Purpose

   Lentiviral Integratable, 4OHT/TMX- and GZV-controllable expression of synZiFTR: KRAB-ZF3

   Depositor

   Ahmad Khalil

   Article

   Li et al Science. 2022 Dec 16;378(6625):1227-1234

   Insert

   pSFFV - KRAB - ZF3 - ERT2 - SMASh
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTS94 pHAGE2 CMVtetO2 GFP-22xGS-3C-MCS

   Plasmid

   #199347

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-3C-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP-3C

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. DVK_AF

    Plasmid

    #66068

    Purpose

    MoClo Destination Vector: (based on pSB1K3) [A:LacZa:F]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Type

    Empty backbone

    Use

    Synthetic Biology; Destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pT3TS-Cre

    Plasmid

    #47117

    Purpose

    Can be used to in vitro transcribe Cre recombinase.

    Depositor

    Stephen Ekker

    Article

    Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

    Insert

    Cre
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Lac

    Available since

    Aug. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDM1489

    Plasmid

    #108996

    Purpose

    Cre expression vector

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Insert

    Cre recombinase
    

    Use

    Cre/Lox; Cre expression vector

    Tags

    Expression

    Mutation

    Promoter

    act15

    Available since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFLIPglu-600uDelta13

    Plasmid

    #12991

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPglu F16A Delta13
    

    Use

    Plant nanosensor

    Tags

    ECFP and EYFP

    Expression

    Plant

    Mutation

    F16A in binding protein

    Promoter

    Available since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

14. WNV NS2B-NS3 protease (based on PDB 2YOL, catalytically active); aliases: West Nile Virus NS2B-NS3 fusion protein

    Plasmid

    #204798

    Purpose

    Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    West Nile NS2B-NS3 fusion
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pBx1-VHH template3-3XMyc-Spacer

    Plasmid

    #176210

    Purpose

    PCR template for generating CeVICA VHH/nanobody library

    Depositor

    Aviv Regev

    Article

    Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

    Insert

    VHH template3-3XMyc-Spacer
    

    Use

    Tags

    Myc tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBx1-VHH template2-3XMyc-Spacer

    Plasmid

    #176209

    Purpose

    PCR template for generating CeVICA VHH/nanobody library

    Depositor

    Aviv Regev

    Article

    Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

    Insert

    VHH template2-3XMyc-Spacer
    

    Use

    Tags

    Myc tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV Syn intron Psam4GlyR m3m4 HA

    Plasmid

    #196041

    Purpose

    AAV for Chemogenetic plasmids

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Insert

    Psam4GlyR m3m4 HA
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMito-dCherry-FRB

    Plasmid

    #186573

    Purpose

    Expression of "dark MitoTrap" - an FRB-domain targeted to the mitochondria that is the same size as pMito-mCherry-FRB but is non-fluorescent - in human cells

    Depositor

    Stephen Royle

    Article

    Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

    Insert

    Dark MitoTrap
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutation of "K70N" to render mCherry no…

    Promoter

    CMV

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMRMTK-clo10

    Plasmid

    #203961

    Purpose

    Cloning plasmid containing the erythromycin resistance gene insert

    Depositor

    Nathan Crook

    Article

    van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

    Insert

    Erythromycin Resistance Gene
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    N/A

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. MSCV Puro-UBC:GFP

    Plasmid

    #68482

    Purpose

    Retrovirus expressing GFP driven by UBC promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Inserts

    Puromycin Resistance
    GFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR and UBC

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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