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pJet1.2 Tev-Avi-tag

Plasmid

#117128

Purpose

C-terminal Tev-Avi tag Tag

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

Tev Avi-tag

Use

Golden gate donor vector for gene targeting in ba…

Tags

Tev Avi-Tag

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTubulin-iRFP670

Plasmid

#197237

Purpose

Expresses the fusion protein of Tubulin-iRFP670 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Tubulin-iRFP670

Expression

Mammalian

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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LP555

Plasmid

#90204

Purpose

pCMV-myc cDNA encoding HsTRAPPC8 aa residues 701-1436

Depositor

Lotte Pedersen

Article

Verdier et al Methods Mol Biol. 2016;1454:15-33. doi:

Insert

TRAPPC8 (TRAPPC8 Human)

Tags

Myc

Expression

Mammalian

Mutation

aa residues 701-1436

Promoter

CMV

Available Since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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FKBP1C-CFP-Cb5

Plasmid

#162438

Purpose

for use in CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

FKBP1C-CFP-Cb5

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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Dendra2-Vinculin-23

Plasmid

#57748

Purpose

Localization: Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Vinculin (VCL Human)

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCytERM-SNIFP-N1

Plasmid

#197189

Purpose

Expresses the fusion protein of CytERM-SNIFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

CytERM-SNIFP

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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aCE120

Plasmid

#204491

Purpose

Figure 1. reporter circuit

Depositor

Ron Weiss

Article

Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

Inserts

EBFP2
mKate2
EYFP

Use

Synthetic Biology

Tags

none

Expression

Bacterial and Mammalian

Promoter

hEF1a

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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C122-E53: mClover3

Plasmid

#162898

Purpose

Standard Gateway entry clone encoding mClover fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Clover3 fluorescent protein

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2+pG10-90

Plasmid

#170879

Purpose

Phytobrick - pG10-90

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

pG10-90

Expression

Plant

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCAH01SpR

Plasmid

#128159

Purpose

pCAH01 derivative (Ptet inducible promoter) with ahp (kanamycin resistance) CDS replaced with the aadA (streptomycin/spectinomycin resistance) CDS

Depositor

Calvin Henard

Article

Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

Type

Empty backbone

Expression

Bacterial

Promoter

Ptet

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pJKR-L-ttgR

Plasmid

#62566

Purpose

Flavonoid biosensor. Low copy plasmid that expresses GFP proportional to flavonoid concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-MBP-yATOM_Ras

Plasmid

#209709

Purpose

Mammalian expression of y-ATOM biosensor for HRas

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

MBP-yATOM_Ras

Tags

MBP

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCytERM-smURFP-N1

Plasmid

#197178

Purpose

Expresses the fusion protein of CytERM-smURFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

CytERM-smURFP

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pOPTM p62 PB1 ZZ (1-167)

Plasmid

#210429

Purpose

Bacterial expression of MBP-PB1 ZZ (1-167) with TEV cleavage site

Depositor

Carsten Sachse

Article

Tarafder et al Methods Mol Biol. 2019;1880:3-15. doi: 1

Insert

p62 PB1 ZZ (1-167) (NUP62 Human)

Tags

MBP

Expression

Bacterial

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAS007

Plasmid

#164868

Purpose

This plasmid can be used to study DNA looping in vivo. pAS007 contains the phiKO2 cB operator sequences but can be modified to insert any DNA operators of interest.

Depositor

Keith Shearwin

Article

Hao et al Nucleic Acids Res. 2021 Jan 28. pii: 612

Insert

phiKO2 operators

Use

Synthetic Biology

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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mEos2-Vinculin-14

Plasmid

#57438

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Vinculin (VCL Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mEos2-Hec1-C-12

Plasmid

#57386

Purpose

Localization: Kinetochore, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Hec1 (NDC80 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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aCE121

Plasmid

#204492

Purpose

Cadherin circuit

Depositor

Ron Weiss

Article

Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

Inserts

EBFP2
Cdh6-mKate2
Cdh1-EYFP

Use

Synthetic Biology

Tags

2A

Expression

Bacterial and Mammalian

Promoter

hEF1a

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pEW65: pQE-81L H3(K79M)

Plasmid

#124100

Purpose

H3K79M expression plasmid for norleucine supplementation

Depositor

Cynthia Wolberger

Article

Worden et al Cell. 2019 Feb 8. pii: S0092-8674(19)301

Insert

Histone H3 (LOC108704296 Frog)

Expression

Bacterial

Mutation

K79M

Promoter

T5

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pET21-tVYN-Nt∆

Plasmid

#154431

Purpose

Plasmid used to express the tVYN-Nt∆ luminescent biosensor for use in live cell cyclic di-GMP detection

Depositor

Ming Hammond

Article

Dippel et al ACS Chem Biol. 2020 Mar 25. doi: 10.1021

Insert

VenusΔC12-NtYcgRΔ-NLucΔ4

Expression

Bacterial

Promoter

T7

Available Since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pRset-ARSeNL

Plasmid

#207872

Purpose

Bacterial expression of the BRET ATP sensor

Depositor

Mathew Tantama

Article

Min et al Sensors (Basel). 2019 Aug 10;19(16). pii

Insert

ARSeNL

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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WZLneo-PPARγ2

Plasmid

#34566

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

Insert

PPARγ2 (Pparg Mouse)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

5'LTR

Available Since

Jan. 25, 2012

Availability

Academic Institutions and Nonprofits only
pRSETb-pHlash

Plasmid

#61550

Purpose

Fusion of Rluc8 and cpVenus. Expression of pHlash in Escherichia coli for protein purification

Depositor

Carl Johnson

Article

Zhang et al PLoS One. 2012;7(8):e43072. doi: 10.1371

Insert

pHlash

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only
pUPD2+pNRP

Plasmid

#170881

Purpose

Phytobrick - pNRP

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

pNRP

Expression

Plant

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pNVLTv2

Plasmid

#169245

Purpose

Generic suicide vector for knockouts and integrations

Depositor

Brian Pfleger

Article

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pTubulin-miRFP713

Plasmid

#197240

Purpose

Expresses the fusion protein of Tubulin-miRFP713 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Tubulin-miRFP713

Expression

Mammalian

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pFUS_A2A

Plasmid

#43849

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

LacZ + BsaI restriction sites

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pFUS_A2B

Plasmid

#43850

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

LacZ + BsaI restriction sites

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-21a(+)-IS200

Plasmid

#12604

Depositor

SeWon Suh

Article

Lee et al J Biol Chem. 2006 Feb 17. 281(7):4261-6.

Insert

IS200 transposase (SSO1474 Sulfolobus solfataricus)

Expression

Bacterial

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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C122-E16: CyPet

Plasmid

#162896

Purpose

Standard Gateway entry clone encoding CyPet fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

CyPet fluorescent protein

Use

Synthetic Biology

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pICSL11197

Plasmid

#191784

Purpose

SpCas9 expression in plants

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

AtUbi10::Cas9 +13 introns-T-E9

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pH2B-miRFP670-2-N1

Plasmid

#197228

Purpose

Expresses the fusion protein of H2B-miRFP670-2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

H2B-miRFP670-2

Expression

Mammalian

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pERB109

Plasmid

#58280

Purpose

constitutive miRFKBP5_Mis12-GFP-FKBP3; inducible mCh-FRB

Depositor

Michael Lampson

Article

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

FKBP-targeted miRNA
Mis12-GFP-FKBPx3
inducible mCh-FRB

Tags

GFP and mCherry

Expression

Mammalian

Available Since

April 6, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pmiRFP680-Actin-C-18

Plasmid

#197219

Purpose

Expresses the fusion protein of miRFP680-Actin-C-18 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP680-Actin-C-18

Expression

Mammalian

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
BASIC_SEVA_5a6.10

Plasmid

#178934

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Article

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Available Since

Feb. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
SIVURA MCP-GFPenvy

Plasmid

#189940

Purpose

Single-integration vector for integration of MCP-GFPEnvy at the URA3 locus in Saccharomyces cerevisiae

Depositor

Tineke Lenstra

Article

Brouwer et al Methods Mol Biol. 2024;2694:235-265. doi

Insert

MCP-GFPEnvy

Expression

Bacterial

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHLK

Plasmid

#64182

Purpose

harbours marker genes HIS3, LEU2 and LYS2

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
TOPO-BC43

Plasmid

#84437

Purpose

Encodes illumina barcodes for allele frequency sequencing (FREQ-Seq-Squared)

Depositor

J.J. Emerson

Article

Zhao et al G3 (Bethesda). 2023 Sep 30;13(10):jkad16

Insert

BC43

Use

Barcoding plasmids

Promoter

SP6/T7

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pERB130

Plasmid

#58284

Purpose

const. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1

Depositor

Michael Lampson

Article

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

Mis12-GFP-FKBPx3
inducible FRB-mCh-Mad1
FKBP-targeted miRNA

Tags

GFP and mCherry

Expression

Mammalian

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBM4607

Plasmid

#18795

Depositor

Mark Johnston

Article

Wang et al Nat Protoc. 2008;3(10):1569-77. doi: 10.

Insert

Sir4 (SIR4 Budding Yeast)

Tags

myc

Expression

Yeast

Mutation

aa 951-1200

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge

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