We narrowed to 16,910 results for: igh

1. pET21a - TR

   Plasmid

   #89621

   Purpose

   [Edit] Expresses recombinant 6HIS tagged T. cruzi trypanothione reductase protein in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   Thioredoxin
   

   Use

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAJM.773

   Plasmid

   #108527

   Purpose

   VanRAM + PVanCC-YFP Reporter. Van sensor for use in non-Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   VanRAM + PVanCC-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBbAW4k-loxP-TT-loxP-mRFP1

   Plasmid

   #134405

   Purpose

   Fluorescent reporter for Cre recombinase activity. Cre removes a transcription terminator, leading to RFP production.

   Depositor

   Mary Dunlop

   Article

   Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

   Insert

   W4-loxP-TT-loxP-mRFP1
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   W4 (modified T7A1)

   Available since

   Jan. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSQT1313

   Plasmid

   #53370

   Purpose

   multiplex gRNA expression plasmid

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   None
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SpCas9-CBE6b-IVT

   Plasmid

   #215834

   Purpose

   Template for in vitro transcription of CBE6b (with SpCas9)

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6b-SpCas9-2xUGI
   

   Use

   In vitro transcription template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pC1.509

   Plasmid

   #203955

   Purpose

   Level 1 self-replicating vector containing a DAPG inducible FnCas12a

   Depositor

   Alistair McCormick

   Article

   Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

   Insert

   PphlF-Cpf1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTn7-SCOUT12.G

   Plasmid

   #201000

   Purpose

   pLv1-pUC18R6KT-miniTn7-Km-sfGFP, KmR, ApR

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Insert

   superfolder GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23104

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBAD/His-miRFP670

   Plasmid

   #79984

   Purpose

   Monomeric near-infrared fluorescent protein miRFP670 in bacterial plasmid

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

   Insert

   miRFP670
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araB

   Available since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA5-TOM20_Halo_T2A_EGFP

   Plasmid

   #135443

   Purpose

   Mamalian cell expression of mictochondrial HaloTag7 and cytosolic EGFP

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

   Insert

   TOM20-HaloTag7 and EGFP
   

   Use

   Tags

   TOM20

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO

   Available since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pWT PolI

    Plasmid

    #11721

    Depositor

    Lawrence Loeb

    Article

    Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

    Insert

    DNA polymerase I (polA E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    wild-type

    Promoter

    Available since

    Aug. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CMV-Citroff1

    Plasmid

    #134304

    Purpose

    Mammalian expression of inverse-response citrate sensor

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    Citroff1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FRP795_insul-(lexA-box)8-PminCYC1-Citrine-TCYC1

    Plasmid

    #58435

    Purpose

    Induces Citrine expression from a promoter containing 8 lexA boxes in yeast cells

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    CitrineA206K
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    insul-(lexA-box)8-PminCYC1

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Myc-TEAD4

    Plasmid

    #24638

    Depositor

    Kunliang Guan

    Article

    Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

    Insert

    TEAD-4 (TEAD4 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJH181.3

    Plasmid

    #135076

    Purpose

    Coryne. glutamicum HA production plasmid

    Depositor

    Josef Altenbuchner

    Article

    Hoffmann et al J Appl Microbiol. 2014 Sep;117(3):663-78

    Insert

    hasA-hasC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHCMV-10A1

    Plasmid

    #15805

    Depositor

    Miguel Sena-Esteves

    Article

    Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

    Insert

    10A1 Murine leukemia virus envelope mRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCHW101020

    Plasmid

    #222454

    Purpose

    Level 1, Position 2 containing 35S::CUP2-p65::NOS.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Insert

    35S::CUP2-p65::NOS (CUP2 )
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    no

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX-4T-1-p19-T7

    Plasmid

    #46306

    Depositor

    Judy Lieberman

    Article

    Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

    Inserts

    p19
    DNA-1 site
    linker
    DNA-2 site
    

    Use

    Pro-sirna

    Tags

    6XHis and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pUC57-gCarvi-T2A

    Plasmid

    #190333

    Purpose

    Fluorescent reporter for cAMP

    Depositor

    Naoto Saitoh

    Article

    Kawata et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

    Insert

    gCarvi
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIVT-mGL

    Plasmid

    #232768

    Purpose

    IVT template for making modRNA encoding mGreenLantern

    Depositor

    Kate Galloway

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mGreenLantern
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTRE-tdTomato

    Plasmid

    #50798

    Purpose

    Expression of tdTomato under the control of the TRE-promoter

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    TREtight driven tdTomato fluorescent protein
    

    Use

    Mutli-species

    Tags

    Expression

    Mutation

    Promoter

    TREtight

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSC101-GFPmut2-mScarlet-I TAA

    Plasmid

    #208187

    Purpose

    nonsense mistranslation, TAA stop

    Depositor

    Tanel Tenson

    Article

    Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

    Inserts

    GFPmut2
    mScarlet-I
    

    Use

    Reporter

    Tags

    Expression

    Bacterial

    Mutation

    Insertion 6TAA*(stop) 7AGT(Ser)

    Promoter

    Ptet+dnaK P1

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCAG-CalfluxCTN

    Plasmid

    #190068

    Purpose

    Expresses BRET Ca2+ sensor CalfluxCTN in mammalian cells

    Depositor

    Carl Johnson

    Article

    Cumberbatch et al Sci Signal. 2022 Aug 16;15(747):eabq7618

    Insert

    CalfluxCTN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRBC-sfGFP wild type

    Plasmid

    #174075

    Purpose

    Plasmid with p15a origin of replication expressing codon optimized superfolder GFP with C-terminal His6 tag, under T7 promoter

    Depositor

    Ryan Mehl

    Article

    Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

    Insert

    Superfolder GFP
    

    Use

    Tags

    His-6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pOsU3

    Plasmid

    #170132

    Purpose

    For sgRNA expression in monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mTagRFP-T-Paxillin-22

    Plasmid

    #58024

    Purpose

    Localization: Focal Adhesions, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Paxillin
    

    Use

    Tags

    mTagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. mApple-pBAD

    Plasmid

    #54536

    Purpose

    Localization: Protein Expression Vector , Excitation: 568, Emission: 592

    Depositor

    Nathan Shaner, Roger Tsien, Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Type

    Empty backbone

    Use

    Tags

    6xHis and mApple

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. SpCas9-CBE6c-IVT

    Plasmid

    #215835

    Purpose

    Template for in vitro transcription of CBE6c (with SpCas9)

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6c-SpCas9-2xUGI
    

    Use

    In vitro transcription template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHR SFFVp Phy-mCherry-CAAX

    Plasmid

    #50839

    Purpose

    Optogenetic PI3K activation system; membrane component

    Depositor

    Wendell Lim

    Article

    Toettcher et al Nat Methods. 2011 Sep 11;8(10):837-9. do

    Insert

    PhyB 1-908
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV-NbALFA-degFusionRed

    Plasmid

    #199466

    Purpose

    Expression of the photodegradable protein degFusionRed fused to an anti-ALFA-tag-specific nanobody in mammalian cells.

    Depositor

    Vladislav Verkhusha

    Article

    Chernov et al Int J Mol Sci. 2023 Mar 30;24(7):6526. d

    Insert

    NbALFA-degFusionRed
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. FRP793_insul-(lexA-box)4-PminCYC1-Citrine-TCYC1

    Plasmid

    #58434

    Purpose

    Induces Citrine expression from a promoter containing 4 lexA boxes in yeast cells

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    CitrineA206K
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    insul-(lexA-box)4-PminCYC1

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHCMV-RabiesG

    Plasmid

    #15785

    Depositor

    Miguel Sena-Esteves

    Article

    Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

    Insert

    Rabies virus G protein (RABVgp4 Rabies virus (strain SAD B19))
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pFPL-Ch

    Plasmid

    #61647

    Purpose

    Gateway vector for generating cyan fluorescent protein fusions in fungi

    Depositor

    Mark Farman

    Article

    Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

    Insert

    enhanced cyan fluorescent protein
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. CMV-mito-CitronRH

    Plasmid

    #158547

    Purpose

    Mitochondria-targeted nonbinding variant of direct-response citrate sensor

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    CitronRH
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pETM6-T7-VHH72

    Plasmid

    #202487

    Purpose

    Expresses the nanobody VHH72 with T7 induction

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody VHH72
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHis17_ParM _1

    Plasmid

    #78201

    Purpose

    Expression of single-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor

    Depositor

    Martin Webb

    Article

    Kunzelmann et al J Biol Chem. 2009 Nov 27;284(48):33130-8

    Insert

    parM (K33A I27C T174A T175N C287A)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    K33A I27C T174A T175N C287A

    Promoter

    unknown

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET30-R5-GFP

    Plasmid

    #17838

    Depositor

    Jay Keasling

    Article

    Shaikh et al Anal Chem. 2008 Feb 1. 80(3):886-90.

    Insert

    pET30-GFP
    

    Use

    Synthetic Biology

    Tags

    His, R5, and S-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Actin-OptoNB

    Plasmid

    #159595

    Purpose

    Opto-Nanobody

    Depositor

    Jared Toettcher, Jose Avalos

    Article

    Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

    Insert

    ActinNB with AsLOV2 at loop 5 with 62-666
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSAM_Bt

    Plasmid

    #112497

    Purpose

    Transposon mutagenesis vector for Bacteroides thetaiotaomicron. Compatible with Insertion Sequencing (INSeq).

    Depositor

    Jeffrey Gordon, Andrew Goodman

    Article

    Goodman et al Cell Host Microbe. 2009 Sep 17;6(3):279-

    Inserts

    INSeq-adapted mariner transposon
    Himar1C9
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFsynW TeTx-LC

    Plasmid

    #197284

    Purpose

    Encodes for viral expression of the light chain of tetanus toxin.

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    Tetanus toxin light chain
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    N/A

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCHW90004

    Plasmid

    #222436

    Purpose

    Level 0, promoter module (GGAG-CCAT) containing CBS4 and tomato miniDFR promoters.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Insert

    CBS4-miniDFR, CBS4 promoter + miniDFR minimal promoter (Solanum lycopersicum)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    no

    Promoter

    Available since

    Nov. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge