We narrowed to 18,708 results for: met

1. pEB2-E2-Crimson

   Plasmid

   #104010

   Purpose

   Plasmid encoding E2-Crimson

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   E2-Crimson
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to DsRed (R2D, S4T, K5E, E10P,…

   Promoter

   proC

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21-pelB-LaG-16-4GS-LaG-2

   Plasmid

   #172764

   Purpose

   Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (4GS linker)

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-16-4GS-LaG-2
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3-U6-sgRNA-PGK-puromycin

   Plasmid

   #51133

   Purpose

   expresses sgRNA from the U6 promoter.

   Depositor

   Xingxu Huang

   Article

   Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDS240

   Plasmid

   #34966

   Purpose

   Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(K533∆, high-affinity epitope).

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   Mid2(SS/TM)-GFP-LOVpep
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   K533∆, high-affinity epitope

   Promoter

   GAL1

   Available Since

   March 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. FH-TET3-pEF

   Plasmid

   #49446

   Purpose

   This plasmid expresses full-length human TET3.

   Depositor

   Anjana Rao

   Article

   Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

   Insert

   Tet methylcytosine dioxygenase 3 (TET3 Human)
   

   Use

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha

   Available Since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221_EGFP

   Plasmid

   #25899

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Insert

   EGFP
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. Salsa6f

   Plasmid

   #140188

   Purpose

   Salsa6f is a fluorescent ratiometric genetically encoded calcium indicator, (tdTomato linked to GCaMP6f by a V5 epitope tag)

   Depositor

   Michael Cahalan

   Article

   Dong et al eLife. 2017 Dec 14;6. pii: 32417. doi: 1

   Insert

   Salsa6f
   

   Use

   Tags

   tdTomato-v5-GCaMP6f

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Immediate Early

   Available Since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Peft-3::cas9-SV40_NLS::tbb-2 3'UTR

   Plasmid

   #46168

   Purpose

   Codon optimized Cas9_SV40 NLS with intron

   Depositor

   John Calarco

   Article

   Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

   Insert

   codon optimized Cas9_SV40 NLS with intron
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   intron inserted at position 3113-3163, and SV40 N…

   Promoter

   eef-1A.1 (eft-3)

   Available Since

   July 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-CRY2-mCherry

   Plasmid

   #58368

   Purpose

   Mamlian codon optimized CRY2

   Depositor

   Won Do Heo

   Article

   Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

   Insert

   CRY2[1-498] (CRY2 Mustard Weed)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAct:dCas9-VPR

    Plasmid

    #78898

    Purpose

    Expresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    pActin (Drosophila)

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. MMW948: CAG-DmrC-NLS-3xFLAG-VPR

    Plasmid

    #104565

    Purpose

    Mammalian expression vector for DmrC fused to VPR activator

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    DmrC-VPR
    

    Use

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMK364 (CMV-OsTIR1-loxP-PURO-loxP)

    Plasmid

    #121184

    Purpose

    OsTIR1 introduction

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    CMV-OsTIR1-loxP-PURO-loxP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    OsTIR1 codon optimized for human cells

    Promoter

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-RAPH1ΔTBS

    Plasmid

    #194859

    Purpose

    Express eGFP-RAPH1 lacking the TBS domain (RAPH1 aa 2-92 deleted) in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

    Insert

    RAPH1 (RAPH1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    RAPH1 with amino acids 2-92 deleted

    Promoter

    CMV

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSFDuet-1_VNp6-mNeongreen

    Plasmid

    #182388

    Purpose

    Bacterial expression of Vesicle Nucleating peptide6-mNeongreen fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp6-mNeongreen-His
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMV-LEU2

    Plasmid

    #65334

    Purpose

    standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker LEU2 which could be released and ligated to series of TUs

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    LEU2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRSFDuet-1_VNp15-mNeongreen

    Plasmid

    #182389

    Purpose

    Bacterial expression of Vesicle Nucleating peptide15-mNeongreen fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp15-mNeongreen-His
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXAT2

    Plasmid

    #80494

    Purpose

    AAVS1 sgRNA expression vector

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    AAVS1 sgRNA-T2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG_mRuby2_smFP FLAG

    Plasmid

    #59760

    Purpose

    encodes multiple copies of an epitope tag FLAG ( not detected by anti GFP ab)

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Insert

    mRuby2_smFP_FLAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Oct. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223_LacZ

    Plasmid

    #25893

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Insert

    LacZ
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFD116

    Plasmid

    #124769

    Purpose

    pFD116 carries dcas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector

    Depositor

    David Bikard

    Article

    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

    Inserts

    Ptet
    dcas9
    PpflB sgRNA BsaI
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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