We narrowed to 10,362 results for: Coli
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Plasmid#65501PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-AFPt9-attR1 and attR2-t7TFPt9-cMycExpressionBacterialPromoterT7 promoterAvailable SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only
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pFLIP35
Plasmid#65502PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-AFPt9-attR1 and attR2-TFPt9-cMycExpressionBacterialPromoterT7 promoterAvailable SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP36
Plasmid#65504PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-AFPt9-attR1 and attR2-Cer-cMycPromoterT7 promoterAvailable SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP42
Plasmid#65617PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-Cit-attR1 and attR2-mCer-cMycExpressionBacterialPromoterT7 promoterAvailable SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SaSrtA-A
Plasmid#64979PurposeExpresses the E108A mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
TagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-59, E108AAvailable SinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SaSrtA-K
Plasmid#64978PurposeExpresses the E105K mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
TagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-59, E105KAvailable SinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (Cow 1-82)
Plasmid#62062PurposeExpression of Cow p53 (residues 1-82) in e. coliDepositorInsertp53
TagsHisExpressionBacterialMutationcontains TAD residues 1-82PromoterT7Available SinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (Rabbit 1-87)
Plasmid#62058PurposeExpression of Rabbit p53 (residues 1-87) in e. coliDepositorInsertp53
TagsHisExpressionBacterialMutationcontains TAD residues 1-87PromoterT7Available SinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL1506
Plasmid#53026PurposePLlacO-1:RBS(st7):csrADepositorInsertcsrA
UseSynthetic BiologyTagsRBS(st7)ExpressionBacterialPromoterpLlac0-1Available SinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-CylI
Plasmid#48200PurposeExpresses CylI (type III PKS) in E. coliDepositorInsertCylI
Tags6x His tagExpressionBacterialPromoterT7Available SinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-29b-CylB
Plasmid#48199PurposeExpresses CylB (acyl carrier protein) in E. coliDepositorInsertCylB
Tags6x His tagExpressionBacterialPromoterT7Available SinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
p70rg 11
Plasmid#17830DepositorInsertE. coli optimized red flourescent protein (RFPec), GFPuv
UseSynthetic BiologyExpressionBacterialAvailable SinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET His10-Draper delta ITAM
Plasmid#126671PurposeExpression of His10-Draper delta ITAM mutant for purification from E. coliDepositorInsertDraper cytoplasmic ITAM residues mutated to Phe
ExpressionBacterialMutationCytoplasmic ITAM residues mutated to PheAvailabilityAcademic Institutions and Nonprofits only -
pAKW.114A-PKA
Plasmid#154051PurposeExpresses PKA in E. coliDepositorInsertProtein kinase cAMP-activated catalytic subunit alpha (PKA) (PRKACA Human)
ExpressionBacterialPromoterpCinAvailabilityAcademic Institutions and Nonprofits only -
piggyBac_rtTA (4th_Gen)_SNCA_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1623)
Plasmid#209085PurposeNgn2 mediated cortical neuron induction, SNCA(wt) expression, Synapsin promoter BirA ligase expressionDepositorExpressionMammalianMutationhuman codon optimizedPromoterTRE4G and hSynAvailable SinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1628)
Plasmid#209086PurposeNgn2 mediated cortical neuron induction, SNCA(Avi tagged) expression, Synapsin promoter BirA ligase expressionDepositorTagsAviTagExpressionMammalianMutationhuman codon optimizedPromoterTRE4G and hSynAvailable SinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
CCLMPC_CAT
Plasmid#237500PurposeDual promoter lentiviral expression control vectorDepositorInsertChloramphenicol acetyl transferase fusion protein
UseLentiviralExpressionMammalianPromoterMND/PGKAvailable SinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
Endy/Shearwin pOSIP plasmid set
Plasmid Kit#1000000035PurposeCloning and assembly of DNA constructs for chromosomal integration in E. coli.DepositorApplicationCloning and Synthetic BiologyVector TypeBacterial ExpressionCloning TypeClonetegrationAvailable SinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-MAT2A
Plasmid#164820PurposeExpresses MAT2A in E. coli BL21(DE3)DepositorAvailable SinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a_T7-ARG2
Plasmid#106474PurposeExpresses acoustic reporter genes or gas vesicles (ARG2) in Escherichia coliDepositorInsertARG2
UseSynthetic BiologyExpressionBacterialPromoterT7Available SinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSEVA221pmCDA1-T7RNAP
Plasmid#167978PurposeExpresses fusion protein pmCDA1-T7RNAP in E. coliDepositorInsertpmCDA1-T7RNAP (T7p07 Synthetic)
TagsT7 tag and T7RNAPExpressionBacterialPromoterTetR-PtetAvailable SinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac2.0
Plasmid#119872PurposeCumate-inducible expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR
sfGFP
UseSynthetic BiologyExpressionBacterialPromoterPveg promoter with CuO sequence and PxylR promoterAvailable SinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac1.8
Plasmid#119871PurposeConstitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR'
sfGFP
UseSynthetic BiologyExpressionBacterialMutationSNP in 592 bpPromoterPserA promoter and Pveg promoter with CuO sequenceAvailable SinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBad-sfGFP
Plasmid#85482PurposeArabinose inducible E. coli codon optimized superfolder GFP with C-terminal His6 tagDepositorInsertsuperfolder GFP
Tags6x HisExpressionBacterialPromoterArabinoseAvailable SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAJF1131
Plasmid#252906PurposeT7-pelB-NBALFA-LE-6his amp (for purification of his-tagged NBALFA from E. coli)DepositorInsertNBALFA
TagsHis Tag and pelB leaderExpressionBacterialPromoterT7Available SinceMarch 30, 2026AvailabilityAcademic Institutions and Nonprofits only -
pDV044
Plasmid#191824PurposePlasmid for the transformation of the cyanobacterium Arthrospira platensis at the KmR locus. pCPC600 promoter. With terminator. Golden Gate compatible. Selection: in A. platensis: KmR; E. coli: AmpRDepositorTypeEmpty backboneUseUnspecifiedAvailable SinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMZ3012
Plasmid#204400PurposeA C4-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.DepositorInsertsUseSynthetic BiologyPromoterPftsH and Synthetic promoter PRhl0L0L12Available SinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-EK_C122S_His5
Plasmid#49048PurposeExpresses the pentahistidine-tagged catalytic domain of bovine enteropeptidase in E. coliDepositorInsertEnteropeptidase, peptidase domain (TMPRSS15 Bovine)
Tags5xHisExpressionBacterialMutationP92R, E185D, C122SPromoterT7Available SinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDB327
Plasmid#113671Purposeexpresses Ulp1_R3 in Escherichia coliDepositorInsertUlp1_R3
Tags6-HisExpressionBacterialMutationcontains solubility enhancing mutationsPromoterT7Available SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-APOBEC1-YTH
Plasmid#200158PurposeFor bacterial expression of the APOBEC1-YTH fusion protein for protein purificationDepositorInsertAPOBEC1-YTH
Tags6xHis, HA, and Maltose Binding ProteinExpressionBacterialMutationCodon optimized for E coli expressionPromoterLacZAvailable SinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-S9
Plasmid#132948PurposeExpresses N-terminal 6xHis-tagged S9 ribosomal protein in E.coliDepositorInsert30S ribosomal subunit protein S9 (rpsI E.coli)
TagsN-terminal 6xHis-tagExpressionBacterialMutationE52CPromoterT7Available SinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPINS-UBE3C
Plasmid#66711PurposeExpresses UBE3C HECT domain in E. coliDepositorAvailable SinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-APOBEC1-YTHmut
Plasmid#200159PurposeFor bacterial expression of the APOBEC1-YTHmut fusion protein for protein purificationDepositorInsertAPOBEC1-YTHmut
Tags6xHis, HA, and MBPExpressionBacterialMutationCodon optimized for E coli expressionPromoterLacZAvailable SinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAC-BETAipi
Plasmid#53277PurposeContains ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coliDepositorInsertidi
UseLow copy number bacterial cloning vectorPromoterendogenous promoterAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAP-BAC
Plasmid#120229PurposeTAR cloning in yeast and low-copy maintenance of large constructs in E. coliDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
VR122 pETv2 10His-pG-Tn5 (E54K, L372P)
Plasmid#198467PurposeE. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in DH5α cloning strain.DepositorInsert10His-pG-Tn5 (E54K, L372P)
Tags10His-pGExpressionBacterialMutationE54K, L372PPromoterConsensus φ10 T7 PromoterAvailable SinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAC-GAMMAipi
Plasmid#53278PurposeHas ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and crtYm gene of marine bacterium P99-3. and produces gamma-carotene in Escherichia coliDepositorInsertcrtYm
UseLow copy number bacterial cloning vectorPromoterendogenous promoterAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/p-bacteria
Plasmid#64325PurposeExpresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPR; Tetracycline-inducibleTags3xFLAGExpressionBacterialMutationD10A, H840APromoterpLtetO-1Available SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIRE4-Bpa
Plasmid#155342PurposePlasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.DepositorInserthumanized EBpaRS/BstYam
ExpressionMammalianPromoterPGK/U6Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pet-MBP
Plasmid#209184PurposeExpression of recombinant proteins with N-terminal MBP-tag and C-terminal Hisx6-tag in E. coliDepositorTypeEmpty backboneTagsHisX6ExpressionBacterialPromoterT7Available SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only