We narrowed to 14,002 results for: flag

1. pScI_dCas9-CDA

   Plasmid

   #108549

   Purpose

   Bacterial Target-AID vector

   Depositor

   Akihiko Kondo

   Article

   Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

   Insert

   dCas9-PmCDA1
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   D10A and H840A for SpCas9

   Promoter

   lambda OR

   Available since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. EcpH-TM

   Plasmid

   #85389

   Purpose

   Expresses ecliptic pHluorin–ICAM-1 (intercellular adhesion molecule 1) in mammalian cells. Incorporates into viral membrane to label pseudoviruses

   Depositor

   Gregory Melikyan

   Article

   Miyauchi et al Biochem J. 2011 Mar 15;434(3):559-69. do

   Insert

   ecliptic pHluorin (EcpH) fused to N-terminus of human ICAM-1 transmembrane domain
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EdTC

   Plasmid

   #24310

   Depositor

   Roel Nusse

   Article

   Fuerer et al PLoS One. 2010 . 5(2):e9370.

   Insert

   EF1alpha-dnhTcf4 // SV40-mCherry (TCF7L2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   31 first amino acids of hTcf4 have been deleted.

   Promoter

   Available since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJK2014

   Plasmid

   #171931

   Purpose

   To express sygl-1 fused to 24 copies of MS2 loops.

   Depositor

   Judith Kimble

   Article

   Lee et al Dev Cell. 2019 Aug 19;50(4):426-435.e4.

   Insert

   Psygl-1::24XMS2 loops::3X Flag::sygl-1 ORF::sygl-1 3'UTR
   

   Use

   Tags

   N-terminal Tag 24X MS2 loops

   Expression

   Worm

   Mutation

   Promoter

   -

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pST1374

   Plasmid

   #13426

   Depositor

   Keith Joung

   Article

   Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

   Type

   Empty backbone

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTriEx-mVenus-Zdk1

   Plasmid

   #81047

   Purpose

   mammalian expression of Zdk1

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Insert

   Zdk1
   

   Use

   Tags

   6x His tag, FLAG, and mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-RILP

   Plasmid

   #74600

   Purpose

   used for expression in E. coli and purification

   Depositor

   Ron Vale

   Article

   Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

   Insert

   Rab-interacting lysosomal protein (RILP Human)
   

   Use

   Tags

   FLAG, StrepII, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pB1H1

   Plasmid

   #12611

   Depositor

   Scot Wolfe

   Article

   Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

   Insert

   Dorsal RHR in pB1H1 (dl Fly)
   

   Use

   Tags

   flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFETCh_ZBTB1

   Plasmid

   #128235

   Purpose

   Donor vector for 3' FLAG tag of human ZBTB1

   Depositor

   Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   ZBTB1 Homology arms
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLentiN 16E6no*

    Plasmid

    #37445

    Purpose

    Lentiviral expression of HPV 16E6no* in mammalian cells

    Depositor

    Karl Munger

    Article

    Spangle et al J Virol. 2012 May 2.

    Insert

    HPV 16E6no* (E6 HPV)
    

    Use

    Lentiviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    V42L

    Promoter

    CMV

    Available since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pGWB701NL3F10H

    Plasmid

    #141288

    Purpose

    No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, Tunicamycin R for plant)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Type

    Empty backbone

    Use

    Luciferase; Gateway

    Tags

    FLAG (x3), HIS (x10), and NLUC

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDest490-OPN-c

    Plasmid

    #17592

    Depositor

    Xin Wang

    Article

    Takafuji et al Oncogene. 2007 Sep 27. 26(44):6361-71.

    Insert

    OPN-c (SPP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Base pairs 1–93, 175–942

    Promoter

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLJM60-Cnbp

    Plasmid

    #38246

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    Cnbp (Cnbp Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pZZMP122

    Plasmid

    #231756

    Purpose

    Mammalian expression of SPO11-YF

    Depositor

    Scott Keeney

    Article

    Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

    Insert

    SPO11-YF (Spo11 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Y138F

    Promoter

    Available since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pZZMP154

    Plasmid

    #231760

    Purpose

    Mammalian expression of LZ-SPO11

    Depositor

    Scott Keeney

    Article

    Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

    Insert

    LZ-SPO11 (Spo11 Budding Yeast, Mouse)
    

    Use

    Tags

    Flag-LeucineZipper

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-T7-SpRY-P2A-mScarlet (MNW002)

    Plasmid

    #174137

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R); C-terminal bi-partite NLS, 3x flag tag, P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpRY with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    SpG=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317…

    Promoter

    CMV and T7

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pVITRO1-SS-338

    Plasmid

    #68795

    Purpose

    Single construct Sensor pair 1, ZF9 Op x6 -- GFP, with mCherry marker

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Inserts

    C-Terminal Sensor 1 V1
    mCherry
    ZF9 Op x6
    EGFP
    N-Terminal Sensor 1 V2
    

    Use

    Tags

    IRES, NLS, flag, and nls

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. lentiMutB3GNT5-blast

    Plasmid

    #208380

    Purpose

    lentiviral vector for expressing human B3GNT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

    Depositor

    Jennifer Kohler

    Insert

    Beta-1,3-N-Acetylglucosaminyltransferase 5 (B3GNT5 Human)
    

    Use

    Lentiviral

    Tags

    myc, FLAG

    Expression

    Mammalian

    Mutation

    nt 922 c->g (PAM site inactivation)

    Promoter

    EF-1 alpha core promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSplit-CO-hDib1

    Plasmid

    #58788

    Purpose

    Express LucC-hDib1 fusion protein in mammalian cells

    Depositor

    Hiroyoshi Ariga

    Article

    Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

    Insert

    hDib1 (TXNL4A Human)
    

    Use

    Tags

    Flag and Luciferase (394-550aa)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKAR1/Pur

    Plasmid

    #23105

    Purpose

    Allows expression of shRNA from U6 promoter and a rescue cDNA from a doxycycline (Tet) inducible promoter in the same plasmid. Contains puromycin resistance gene

    Depositor

    Randy Poon

    Article

    Ma et al Nucleic Acids Res. 2007 . 35(4):e22.

    Type

    Empty backbone

    Use

    RNAi

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    TRE for cDNA; U6 for shRNA

    Available since

    May 27, 2011

    Availability

    Academic Institutions and Nonprofits only

21. pNCMV 16E6no*

    Plasmid

    #37454

    Purpose

    Expression of HPV 16E6no* in mammalian cells

    Depositor

    Karl Munger

    Article

    Spangle et al J Virol. 2012 May 2.

    Insert

    HPV 16E6no* (E6 HPV)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    V42L

    Promoter

    CMV

    Available since

    Sept. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

22. pCDNA-FRT-FAK30

    Plasmid

    #59121

    Purpose

    FAK fret sensor with 30 nm helix

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Use

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSPlit-OC-hDib1

    Plasmid

    #58789

    Purpose

    Express hDib1-LucC fusion protein in mammalian cells

    Depositor

    Hiroyoshi Ariga

    Article

    Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

    Insert

    hDib1 (TXNL4A Human)
    

    Use

    Tags

    Flag and Luciferase (394-550aa)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

24. pSplit-ON-hDib1

    Plasmid

    #58787

    Purpose

    Express hDib1-LucN fusion protein in mammalian cells

    Depositor

    Hiroyoshi Ariga

    Article

    Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

    Insert

    hDib1 (TXNL4A Human)
    

    Use

    Tags

    Flag and Luciferase (1-398aa)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

25. PylT(UAG) PylS mCherry-P2A-eGFP(150TAG)

    Plasmid

    #174526

    Purpose

    Dual-fluorescence reporter for quantifying PylT(UAG) decoding efficiency

    Depositor

    Yu-Hsuan Tsai

    Article

    Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

    Insert

    PylS
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLJM1-PCYT2

    Plasmid

    #81074

    Purpose

    Expresses PCYT2 in mammalian cells

    Depositor

    Erin O'Shea

    Article

    Chidley et al Elife. 2016 Jul 12;5. pii: e14601. doi:

    Insert

    phosphate cytidylyltransferase 2, ethanolamine (PCYT2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCS2-FAK30

    Plasmid

    #59131

    Purpose

    Cloning vector for FAK fret sensor with 30 nm helix

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Use

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTL2-IncB-GFP11x7

    Plasmid

    #113031

    Purpose

    Expresses IncB tagged with 7 copies of GFP11 under a tetracycline inducible promoter

    Depositor

    Kevin Hybiske

    Article

    Wang et al Pathog Dis. 2018 Mar 1;76(2). pii: 48301

    Insert

    IncB (incB Chlamydia trachomatis)
    

    Use

    Tags

    GFP11x7 and flag

    Expression

    Bacterial

    Mutation

    Promoter

    tet inducible

    Available since

    Oct. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pOC4 cloning template vector

    Plasmid

    #183423

    Purpose

    FlpON knock-in vector with FLAG-SpCas9 expression

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLJM60-eIF4E

    Plasmid

    #38239

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    eIF4E (Eif4e Rat)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCDNA-FRT-FAK10

    Plasmid

    #59119

    Purpose

    FAK fret sensor with 10 nm helix

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Use

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCS2-FAK0

    Plasmid

    #59128

    Purpose

    Cloning vector for FAK fret sensor without helix

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Use

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAH237

    Plasmid

    #121146

    Purpose

    Expression of both nmt41p-cas9 and rrk1p-gRNA (LEU2 marker) for CRISPR genome editing in fission yeast

    Depositor

    Katsunori Tanaka

    Article

    Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

    Inserts

    gRNA cassette
    humanized Streptococcus pyogenes Cas9
    

    Use

    CRISPR

    Tags

    Flag

    Expression

    Yeast

    Mutation

    Promoter

    S.pombe rrk1 and nmt41

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGWB601NL3F10H

    Plasmid

    #141287

    Purpose

    No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, BASTA R for plant)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Type

    Empty backbone

    Use

    Luciferase; Gateway

    Tags

    FLAG (x3), HIS (x10), and NLUC

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pKS014

    Plasmid

    #65467

    Purpose

    pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and beta-galactosidase

    Depositor

    Keith Tyo

    Article

    Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

    Insert

    Ntag-beta galactosidase
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    pl-TetO

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

36. Halo-Sox13

    Plasmid

    #200194

    Purpose

    Expression of full length Sox13

    Depositor

    Sheila Teves

    Article

    Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

    Insert

    Halo-Sox13 (Sox13 Mouse)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    None

    Promoter

    EF_1alpha

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDD2701

    Plasmid

    #175078

    Purpose

    Mammalian expression of Flag-tagged KSHV ORF45

    Depositor

    Dirk Dittmer

    Article

    Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

    Insert

    KSHV ORF45 (ORF45 Kaposi's sarcoma-associated herpesvirus 8 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEPIC1.1

    Plasmid

    #169897

    Purpose

    Empty constitutive expression vector with piggyBac, IRES, and mClover3

    Depositor

    Richard Schneider

    Article

    Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

    Type

    Empty backbone

    Use

    Avian expression

    Tags

    3x FLAG 2x StrepII

    Expression

    Mammalian

    Mutation

    Promoter

    mouse PGK

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEntr-Nkx2-5flbio

    Plasmid

    #32969

    Depositor

    William Pu

    Article

    He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

    Insert

    Nkx2-5 (Nkx2-5 Mouse)
    

    Use

    Gateway

    Tags

    flag-bio

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLIA-E p27 (CC#620)

    Plasmid

    #15191

    Depositor

    Connie Cepko

    Article

    Dyer et al J Neurosci. 2001 Jun 15. 21(12):4259-71.

    Insert

    p27 (Cdkn1b Mouse)
    

    Use

    Retroviral

    Tags

    Flag and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only

41. pCMV-T7-SpCas9-NG-P2A-mScarlet (MNW005)

    Plasmid

    #174140

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…

    Promoter

    CMV and T7

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. TAL2472

    Plasmid

    #36853

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    TP53-TALEN_Right (Tp53 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

43. TAL2348

    Plasmid

    #36768

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    TP53-TALEN_Left (Tp53 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

44. pCDNA-FRT-FAK20

    Plasmid

    #59120

    Purpose

    FAK fret sensor with 20 nm helix

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Use

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCDNA-FRT-FAK0

    Plasmid

    #45047

    Purpose

    FAK fret sensor without helix

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Use

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. HOXA9-MSCV-full-GFP

    Plasmid

    #20977

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol. 2008 Jul . 28(14):4609-19

    Insert

    HOXA9 (Hoxa9 Mouse)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Full length cDNA with ~1.1kb 3'UTR with 2 pu…

    Promoter

    Available since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pGWB401NL3F10H

    Plasmid

    #141285

    Purpose

    No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, KmR for plant)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Type

    Empty backbone

    Use

    Luciferase; Gateway

    Tags

    FLAG (x3), HIS (x10), and NLUC

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. LPCX Smad3 deltaSSVS

    Plasmid

    #12639

    Depositor

    Rik Derynck

    Article

    Choy et al J Cell Biol. 2000 May 1. 149(3):667-82.

    Insert

    Smad3 (SMAD3 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Last 4 aa's deleted.

    Promoter

    Available since

    Oct. 10, 2006

    Availability

    Academic Institutions and Nonprofits only

49. pMIG-Aalpha E64G

    Plasmid

    #10885

    Depositor

    William Hahn

    Article

    Chen et al Cancer Res. 2005 Sep 15. 65(18):8183-92.

    Insert

    PP2A Aalpha (PPP2R1A Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    E64G

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

50. pKS011

    Plasmid

    #65464

    Purpose

    pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) MBP (Maltose Binding Protein), Ntag, and mCherry

    Depositor

    Keith Tyo

    Article

    Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

    Insert

    MBP-Ntag-mCherry
    

    Use

    Tags

    FLAG and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pl-TetO

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge