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Showing: 5161 - 5190 of 160338 results

  1. pEP4 E02S ET2K

    Plasmid
    #20927
    Purpose
    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; SV40LT and Klf4
    Depositor
    James Thomson
    Article
    Yu et al Science. 2009 Mar 26.
    Insert
    Oct4 and Sox2; SV40LT and Klf4 (SOX2, POU5F1, KLF4 Human)
    Use
    Episomal/ebna
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 20, 2009
    Availability
    Academic Institutions and Nonprofits only
    20927-plasmid-map-sequence-id-9763

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  2. GPX4

    Plasmid
    #38797
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GPX4 (GPX4 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    SeCys73 -> Gly
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    38797-plasmid-map-sequence-id-428950

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  7. pcDNA-dCas9-p300 Core (D1399Y)

    Plasmid
    #61358
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61358-plasmid-map-sequence-id-99789

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  10. pRRL.SIN.EF1A.CD19-FMC63.218.CAR-3G

    Plasmid
    #194458
    Purpose
    CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.
    Depositor
    Ana Sofia Coroadinha
    Article
    Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:
    Insert
    Anti-CD19 CAR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    194458-plasmid-map-sequence-id-384169

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  11. pCAG-eCas9-GFP-U6-gRNA

    Plasmid
    #79145
    Purpose
    All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in human pluripotent stem cells
    Depositor
    Jizhong Zou
    Insert
    eSpCas9(1.1)
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    79145-plasmid-map-sequence-id-182555

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  14. pAAV-Ef1a-DIO ChETA-EYFP (AAV9)

    Viral Prep
    #26968-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-Ef1a-DIO ChETA-EYFP (#26968). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO ChETA-EYFP plasmid DNA. EF1a-driven, Cre-dependent, ChETA fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  16. pGP-AAV-syn-jGCaMP7f-WPRE (AAV Retrograde)

    Viral Prep
    #104488-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pGP-AAV-syn-jGCaMP7f-WPRE (#104488). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7f-WPRE plasmid DNA. Synapsin-driven GCaMP7f calcium sensor. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project, Douglas Kim
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Promoter
    Syn
    Tags
    None
    Available since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only

  22. Mouse sgRNA library Brie in lentiGuide-Puro (Lentiviral Prep)

    Viral Prep
    #73633-LV
    Purpose
    Ready-to-use Lentiviral Prep particles produced from Mouse sgRNA library Brie in lentiGuide-Puro (#73633). In addition to the viral particles, you will also receive purified Mouse sgRNA library Brie in lentiGuide-Puro plasmid DNA. <p>&lt;p&gt;Ready-to-use lentiviral pooled library for CRISPR screening in mouse cells. Use on cells that are stably expressing Cas9 to make edits across 19,674 genes in the mouse genome.&lt;/p&gt;</p>
    Depositor
    David Root, John Doench
    Article
    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103
    Promoter
    Tags
    None
    Available since
    Aug. 16, 2016
    Availability
    Academic Institutions and Nonprofits only

  24. Lamp1-mScarlet-I

    Plasmid
    #98827
    Purpose
    In vivo visualization of lysosomes (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    Lamp1
    Use
    Tags
    mScarlet-I
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98827-plasmid-map-sequence-id-430619

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  25. pENN.AAV.CMVs.Pl.Cre.rBG (AAV2)

    Viral Prep
    #105537-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pENN.AAV.CMVs.Pl.Cre.rBG (#105537). In addition to the viral particles, you will also receive purified pENN.AAV.CMVs.Pl.Cre.rBG plasmid DNA. CMV-driven Cre. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CMV
    Tags
    None
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only

  26. pS-cr:GFP

    Plasmid
    #196295
    Purpose
    35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding crRNA:GFP fusion in place of the NSs
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV S antigenome encoding crRNA:GFP fusion in place of viral NSs
    Use
    CRISPR
    Tags
    SpeI-DR-BsaI-DR-SpeI
    Expression
    Plant
    Mutation
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196295-plasmid-map-sequence-id-390035

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  29. PB CAG-sfGFP

    Plasmid
    #133568
    Purpose
    CAG sfGFP piggyBac transposon
    Depositor
    Mario Capecchi
    Insert
    sfGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133568-plasmid-map-sequence-id-274027

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  30. pET-19b_MMLV-RT

    Plasmid
    #153312
    Purpose
    Bacterial expression of MMLV reverse transcriptase
    Depositor
    Drew Endy, Philippa Marrack
    Insert
    MMLV Reverse Transcriptase
    Use
    Tags
    10x His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 2, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153312-plasmid-map-sequence-id-298742

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Showing: 5161 - 5190 of 160338 results