163,403 results

1. Cas9 TLS2

   Plasmid

   #196978

   Purpose

   Expresses Cas9 fused with TLS2 mobility signal that confers graft mobility. Induced by estradiol.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   Cas9 with TLS2 tag
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pG10-90

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB-CAG-dCas9-VPR-wpA-pPuroTK

   Plasmid

   #214127

   Purpose

   Gene expression by Piggybac transposase in mammalian cells

   Depositor

   Sho Yoshimatsu

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Codon-optimized for mammalian cells

   Promoter

   Available since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOPTN-EGFP

   Plasmid

   #27052

   Depositor

   Beatrice Yue

   Article

   Park et al Am J Pathol. 2006 Dec . 169(6):1976-89.

   Insert

   optineurin (OPTN Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. multi-shRNA vector

   Plasmid

   #12391

   Depositor

   Zuoshang Xu

   Article

   Xia et al Biotechniques. 2006 Jul . 41(1):64-8.

   Insert

   mSOD2 shRNA (Sod2 Mouse)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. mEmerald-CLIP170-C-18

   Plasmid

   #54043

   Purpose

   Localization: Microtubule Binding , Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   CLIP170 (CLIP1 Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 2, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pDONR223_STAT3_WT

   Plasmid

   #82235

   Purpose

   Gateway Donor vector containing STAT3, part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Jesse Boehm, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   STAT3 (STAT3 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. p2attPC

   Plasmid

   #51547

   Purpose

   DICE donor plasmid without neural transcription factors.

   Depositor

   Michele Calos

   Article

   Zhu et al Nucleic Acids Res. 2013 Dec 4.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF1652

   Plasmid

   #143532

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SPI1 (SPI1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF1α-Cre-2A-moxGFP

   Plasmid

   #207744

   Purpose

   Expression of the Cre recombinase linked to a moxGFP fluorescent marker

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   Cre-PGK-moxGFP
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. FUG-Pten

    Plasmid

    #140732

    Purpose

    Lentiviral vector to overexpress wild-type human PTEN as a GFP-PTEN fusion protein

    Depositor

    Bryan Luikart

    Article

    Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

    Insert

    GFP-Pten (PTEN Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    hUbiC

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Cas9 sgRNA vector

    Plasmid

    #68463

    Purpose

    Cas9 sgRNA vector for cloning

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-CreERT2

    Plasmid

    #14797

    Purpose

    Mammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Cre-ERT2 fusion protein
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. Tol2-kdrl:mCherry-caax

    Plasmid

    #194287

    Purpose

    Expression plasmid for zebrafish or Danionella cerebrum

    Depositor

    Pui-Ying Lam

    Article

    Lam et al Dis Model Mech. 2022 Dec 1;15(12):dmm049

    Insert

    kdrl promoter from Addgene plasmid # 78687 (kdrl Zebrafish)
    

    Use

    Zebrafish or danionella cerebrum expression

    Tags

    mCherryCAAX from the Tol2kit (Kwan et al., 2007)

    Expression

    Mutation

    Promoter

    kdrl(flk1)

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. TERT - WT

    Plasmid

    #213827

    Purpose

    Expresses human telomerase reverse transcriptase, the catalytic subunit of telomerase

    Depositor

    Coleman Lindsley

    Article

    Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

    Insert

    human TERT (TERT Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-responsive promoter PTight, consisting of sev…

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. mEmerald-mDia1-N-14

    Plasmid

    #54157

    Purpose

    Localization: Cytoskeleton, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    mDia1 (DIAPH1 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pcDNA3.1 SARS-CoV-2 N

    Plasmid

    #158079

    Purpose

    Expresses SARS-CoV-2 nucleocapsid protein in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 nucleocapsid (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-GGamma11-GFP2

    Plasmid

    #166780

    Purpose

    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma11-GFP2 (GNG11 Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains an N-terminal GFP2 and GSAG linker

    Promoter

    CMV

    Available since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti6M GLUT3

    Plasmid

    #194139

    Purpose

    Expresses GLUT3 protein in mammalian cells

    Depositor

    Jun Liu

    Insert

    SLC2A3 (SLC2A3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pExp-xMBP-ACE2-CHis

    Plasmid

    #194998

    Purpose

    Production of ACE2 extracellular domain

    Depositor

    Marko Hyvönen

    Article

    Harman et al J Med Chem. 2023 Jul 27;66(14):9881-9893

    Insert

    ACE2 (ACE2 Human)
    

    Use

    Tags

    His8 and xMBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac

    Available since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Human_TFAM_NoMTS_pET28

    Plasmid

    #34705

    Depositor

    David Chan

    Article

    Ngo et al Nat Struct Mol Biol. 2011 Oct 30;18(11):

    Insert

    TFAM (TFAM Human)
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Encodes residues 43–246, corresponding to full-le…

    Promoter

    T7

    Available since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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21. pcDNA3.1-GGamma10-GFP2

    Plasmid

    #166779

    Purpose

    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma10-GFP2 (GNG10 Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains an N-terminal GFP2 and GSAG linker

    Promoter

    CMV

    Available since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHMGWA-Pa14Csy4

    Plasmid

    #41091

    Depositor

    Jennifer Doudna

    Article

    Haurwitz et al Science. 2010 Sep 10;329(5997):1355-8.

    Insert

    Csy4
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    Wild type

    Promoter

    T7

    Available since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

23. pAAV-CA

    Plasmid

    #69616

    Purpose

    To express gene strongly with AAV

    Depositor

    Naoshige Uchida

    Article

    Menegas et al Elife. 2015 Aug 31;4. doi: 10.7554/eLife

    Type

    Empty backbone

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDONR223_LacZ

    Plasmid

    #25893

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Insert

    LacZ
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-hSyn-miniDq-P2A-mCherry

    Plasmid

    #204357

    Purpose

    AAV vector for miniDq expression under the control of human synapsin promoter

    Depositor

    Masao Doi

    Article

    Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

    Insert

    miniDq-P2A-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    The third intracellular loop (ICL3) of hM3Dq was …

    Promoter

    hSyn

    Available since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHMGWA-Pa14Csy4H29A

    Plasmid

    #41092

    Depositor

    Jennifer Doudna

    Article

    Haurwitz et al Science. 2010 Sep 10;329(5997):1355-8.

    Insert

    Csy4
    

    Use

    Tags

    His6-MBP

    Expression

    Mutation

    His29Ala mmutation to abolish ribonuclease activi…

    Promoter

    T7

    Available since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

27. pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s (AAV5)

    Viral Prep

    #105715-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s (#105715). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s plasmid DNA. EF1a-driven, Cre-dependent GCaMP6s fused to the presynaptic protein synaptophysin. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Rylan Larsen

    Promoter

    Ef1a, Human elongation factor-1 alpha

    Tags

    None

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

28. pLenti-puro-ARID1A

    Plasmid

    #39478

    Purpose

    Tetracycline-inducible lentiviral expression of human ARID1A; 3rd generation lentiviral vector

    Depositor

    Ie-Ming Shih

    Article

    Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

    Insert

    ARID1A (ARID1A Human)
    

    Use

    Lentiviral

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pYTK001

    Plasmid

    #65108

    Purpose

    Part Plasmid Entry Vector to be used in the Dueber YTK system.

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    none
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PX458 eSp(1.1) V3

    Plasmid

    #226963

    Purpose

    CBh-eSpCas9(1.1)-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pWSK29

    Plasmid

    #172972

    Purpose

    Low copy number cloning vector

    Depositor

    Renato Morona

    Article

    Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. V5-GFP-TurboID-KDEL_pLX208

    Plasmid

    #202020

    Purpose

    expresses GFP-TurboID in the mammalian ER lumen, lentiviral vector

    Depositor

    Alice Ting

    Article

    Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

    Insert

    V5-TurboID-KDEL
    

    Use

    Lentiviral

    Tags

    GFP and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCDNA3.1_Zeo_TetO_FLAG-AT1R

    Plasmid

    #222856

    Purpose

    Mammalian expression plasmid for FLAG-tagged AT1R

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT1R (AGTR1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLVX-Cry2olig

    Plasmid

    #208780

    Purpose

    Inducibly expresses light activatable Cry2 domain

    Depositor

    Petr Broz

    Article

    Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    Cry2 mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3.1-Gαi*-BERKY3

    Plasmid

    #158198

    Purpose

    BRET biosensor for detection of Gαi-GTP in mammalian expression vector pcDNA3.1(+)

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. MSCV-EZH2-PGK-Puro-IRES-GFP

    Plasmid

    #75125

    Purpose

    Retroviral expression plasmid encoding wild type human EZH2

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    KMT6 (EZH2 Human)
    

    Use

    Retroviral

    Tags

    None

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

37. pcDNA5/FRT/TO_Beta4Gal-HaloTag7

    Plasmid

    #175536

    Purpose

    Expression of HaloTag7 at the at the Gogli apparatus of the mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Beta4Gal-HaloTag7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCAGGS-Flag-hsDicer

    Plasmid

    #41584

    Depositor

    Phil Sharp

    Article

    Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

    Insert

    hsDicer (DICER1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    Jan. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-P3-Alb-mScarlet

    Plasmid

    #183461

    Purpose

    To produce AAV with the P3 promoter to express Alb(mouse)-mScarlet fusion protein

    Depositor

    Hajime Hirase

    Article

    Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

    Insert

    Alb (Alb Mouse)
    

    Use

    AAV

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pIVM_02

    Plasmid

    #204500

    Purpose

    Expression of VHL (54-213) for production of VCB complexes in conjunction with plasmid pIVM_26 (EloB 1-104, EloC 17-112)

    Depositor

    Alessio Ciulli

    Article

    Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

    Insert

    amino acids 54-213 VHL (VHL Human)
    

    Use

    Tags

    6xHis, TEV, and Thrombin

    Expression

    Bacterial

    Mutation

    0

    Promoter

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge