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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 18,722 results for: IRE

Showing: 8901 - 8950 of 18722 results

  1. pCS2+-Zeo-miR-430-10-11 mm

    Plasmid
    #221727
    Purpose
    Expression of Zeocin resistance marker followed by miR-430 target site insertion with mismatches at position 10 and 11 in zebrafish embryos.
    Depositor
    David Bartel
    Article
    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5
    Insert
    BleoR-miR-430-10-11 mm
    Use
    Fish expression
    Tags
    Expression
    Mutation
    Mismatch at positions 10 and 11 of miR-430 site
    Promoter
    simian CMV IE94
    Available since
    Dec. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    221727-plasmid-map-sequence-id-449656

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  2. mCit(P5f-P13f)3-gs

    Plasmid
    #228080
    Purpose
    Mammalian expression of coiled coil segments (P5f-P13f)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCit, P5f, P13f
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    228080-plasmid-map-sequence-id-454693

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  3. mCit(S1h)3(S3h)3-gs

    Plasmid
    #228079
    Purpose
    Mammalian expression of coiled coil segments (S1h)x3-(S3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCit, S1h, S3h
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    228079-plasmid-map-sequence-id-454689

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  4. mCit(S1h-S3h)3-gs

    Plasmid
    #228076
    Purpose
    Mammalian expression of coiled coil segments (S1h-S3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCit, S1h, S3h
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    228076-plasmid-map-sequence-id-454655

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  5. RFP-PPPPPPAAAA-gs

    Plasmid
    #228092
    Purpose
    Mammalian expression of 10 coiled coil segments in PPPPPPAAAA arrangement, with short flexible linkers and N-terminal mCitrine for phase separation and forming of liquid condensates in mammalian cells
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCit, PPPPPPAAAA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    228092-plasmid-map-sequence-id-451390

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  6. mCit(D1h-D3h)3-gs

    Plasmid
    #228088
    Purpose
    Mammalian expression of coiled coil segments (D1h-D3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCit, D1h, D3h
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    228088-plasmid-map-sequence-id-451399

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  8. TagBFP(S2h)12-gs

    Plasmid
    #228087
    Purpose
    Mammalian expression of coiled coil segments (S2h)x12, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    BFP, S2h
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    228087-plasmid-map-sequence-id-451132

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  9. TagBFP(S2h)3(S4h)3-gs

    Plasmid
    #228077
    Purpose
    Mammalian expression of coiled coil segments (S2h)x3-(S4h)x3, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    BFP, S2h, S4h
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    228077-plasmid-map-sequence-id-451130

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  12. pDUP2

    Plasmid
    #223517
    Purpose
    cogfp copy 1 under control of Ptet, cogfp copy 2 under control of Ptac
    Depositor
    Yolanda Schaerli
    Article
    Ljiljana et al eLife13:RP97216
    Inserts
    CoGFP
    CoGFP (second copy)
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    anti-dimerization mutations S129G, 22 C154S, D156…
    Promoter
    Ptac, Ptac and Ptet, and Ptet
    Available since
    Sept. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    223517-plasmid-map-sequence-id-444179

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Showing: 8901 - 8950 of 18722 results