We narrowed to 19,393 results for: IRE

Showing: 8901 - 8950 of 19393 results

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pET21-pelB-LaG-29

Plasmid

#172753

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-29; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-29

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-FRT-TO-GFP-PALB2 KR

Plasmid

#71107

Purpose

mammalian expression of GFP-PALB2 mutant. (first 8 Lysine residues mutated to Arginine)

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

PALB2 (PALB2 Human)

Tags

GFP

Expression

Mammalian

Mutation

first 8 Lysine residues mutated to Arginine (K14-…

Promoter

CMV/TO

Available Since

Dec. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pZeo-Cas9-gate

Plasmid

#113744

Purpose

Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving Zeocin for selection in plants.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

Cas9

Use

CRISPR; Gateway destination vector

Promoter

Z. Mays ubiquitin promoter, 35S promoter

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-MYB-3'UTR

Plasmid

#25798

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

3'UTR of human MYB (MYB Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsMago_I

Plasmid

#146582

Purpose

Mammalian Expression of HsMago

Depositor

Elisa Izaurralde

Article

Palacios et al Nature. 2004 Feb 19. 427(6976):753-7.

Insert

HsMago (MAGOH Human)

Expression

Mammalian

Mutation

one non silent mutation Y123C compared to the seq…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pHisAlix

Plasmid

#42576

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

ALIX (PDCD6IP Human)

Tags

HIS-TEV

Expression

Bacterial

Mutation

silent mutation A1617G (numbering in ALIX gene)

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only
pUpstream2Lox

Plasmid

#164573

Purpose

Integration of 2 LoxN sites in the genome of P. berghei

Depositor

Olivier Silvie

Inserts

Green fluorescent protein - human dihydrofolate reductase (GFP-2A-hDHFR) (DHFR Synthetic, Human)
bifunctional yeast enzyme cytosine deaminase and uridyl phosphoribosyl transferase (yFCU) (FCY1, FUR1 Budding Yeast)

Use

Cre/Lox

Promoter

Bidirectional PbEF1alpha promoter (PBANKA_1133300…

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pJRoC30_VP5H

Plasmid

#188061

Purpose

PROSS-designed versatile peroxidase 5H

Depositor

Sarel Fleishman

Article

Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35

Insert

PROSS-designed versatile peroxidase from Pleurotus ostreatus

Tags

S. cerevisiae native α-factor prepro-leader signa…

Expression

Yeast

Mutation

43 PROSS mutations, described in publication

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3/mArf3(T31N)-HA

Plasmid

#79416

Purpose

Expresses C-terminally HA-tagged Arf3(T31N) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

Insert

Arf3 (Arf3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

changed Threonine 31 to Asparagine

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3/mArf3(Q71L)-HA

Plasmid

#79415

Purpose

Expresses C-terminally HA-tagged Arf3(Q71L) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

Insert

Arf3 (Arf3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

changed Glutamine 71 to Leucine

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pGoldyTALEN-VIM-TAL-2

Plasmid

#62852

Purpose

TALEN mediated gene editing at human VIM locus

Depositor

Gaudenz Danuser

Article

Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

Insert

TALEN array

Use

TALEN

Expression

Mammalian

Promoter

miniCAGG

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only

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pGoldyTALEN-VIM-TAL-1

Plasmid

#62855

Purpose

TALEN mediated gene editing at human VIM locus

Depositor

Gaudenz Danuser

Article

Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

Insert

TALEN array

Use

TALEN

Expression

Mammalian

Promoter

miniCAGG

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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FLAG-mFzd4-ΔC(40-513)-3C-sGFP1-10_pVL1393

Plasmid

#216380

Purpose

Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 ΔC (residues 40-513) fused to split GFP (strands 1-10)

Depositor

William Weis

Article

Bruguera et al bioRxiv 2024.02.03.578714

Insert

Frizzled-4 (Fzd4 Mouse)

Use

Baculovirus

Tags

3C-splitGFP1to10opt-ETVV and HA signal sequence-F…

Expression

Insect

Promoter

Polyhedrin

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pPotef-cdc3CB16-NatR

Plasmid

#86465

Purpose

Expresses an N-terminal Cdc3-mCherry fusion under the control of the constitutive Potef promotor. 16boxB stem-loops are integrated into the 3'UTR to visualize the mRNA.

Depositor

Michael Feldbrügge

Article

Baumann et al EMBO Rep. 2014 Jan;15(1):94-102. doi: 10

Insert

Cdc3

Use

Ustilago maydis expression

Tags

mCherry

Expression

Bacterial

Promoter

Potef

Available Since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-17

Plasmid

#172747

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-17; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-17

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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RCAS(A) mPea3 EnR (CT#1120)

Plasmid

#13909

Depositor

Cliff Tabin

Article

Brent et al Development. 2004 Aug . 131(16):3885-96.

Insert

Pea3 (Etv4 Mouse)

Use

Retroviral; Avian expression

Available Since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
pXD70CT5-PxylR-CymR, Pveg(CuO)-LacZ

Plasmid

#191627

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5 (engineered from pCT5-bac2.0)-LacZ

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

lacZ

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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p1977 (pUC19-PTDH3-PTEF1)

Plasmid

#78230

Purpose

Example plasmid containing a bidirectional promoter of pTDH3 and pTEF1

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

Bidirectional promoters pTDH3-pTEF1

Expression

Bacterial

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only
pSLIK_hygro_3xflag_Luciferase

Plasmid

#136528

Purpose

Lentiviral expression vector for an inducible 3xflag-tagged Luciferase

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

Luciferase

Use

Lentiviral; Destinatioin vector for gateway cloni…

Tags

3x FLAG

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Flag-hsDicer (E1564A)

Plasmid

#41587

Depositor

Phil Sharp

Article

Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

Insert

hsDicer (DICER1 Human)

Tags

Flag

Expression

Mammalian

Mutation

E1564A

Promoter

CMV

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-p97wt-mycStrep

Plasmid

#31837

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Tags

Strep and myc

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only
pFRTpsiCHECK

Plasmid

#154454

Purpose

Expression of renilla luciferase and firefly luciferase

Depositor

Markus Landthaler

Article

Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

Insert

Renilla luciferase, firefly luciferase

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSicoR human Dicer3

Plasmid

#14765

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Dicer shRNA (DICER1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
pBOB-CARMIL2 BH domain-tdTomato

Plasmid

#118737

Purpose

To express CARMIL2 tagged BH with tdTomato on C-terminus

Depositor

John Cooper

Article

Lanier et al J Biol Chem. 2016 Jan 15;291(3):1076-91.

Insert

CARMIL2 BH domain (CARMIL2 Human)

Use

Lentiviral

Tags

tdTomato

Promoter

CMV

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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prp8-G736E

Plasmid

#108450

Purpose

prp8-G736E in pRS313 backbone for expression in yeast

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2019 Feb 5;116

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

changed G736 to E

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pGL2-100bp

Plasmid

#16294

Depositor

Wafik El-Deiry

Article

Wang et al Cancer Res. 2006 Jul 15. 66(14):6982-9.

Insert

p53 promoter fragment (TP53 Human)

Use

Luciferase

Expression

Mammalian

Mutation

fragment -88 to +12 relative to first transcripti…

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAF211

Plasmid

#52407

Purpose

Expresses Flag-tagged mouse Arf6

Depositor

Gregory Pazour

Article

Follit et al PLoS Genet. 2014 Feb 20;10(2):e1004170.

Insert

Arf6 (Arf6 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pGEMT-Map7-1333-end

Plasmid

#46070

Depositor

Edgar Gomes

Article

Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

Insert

Map7-1333-end (Map7 Mouse)

Expression

Bacterial

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-TTF-1-HDD

Plasmid

#52063

Purpose

It encodes a mutant of TTF-1 in which the homeodomain is deleted

Depositor

David Mu

Article

Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

Insert

Thyroid Transcription Factor 1 (NKX2-1 Human)

Expression

Mammalian

Mutation

Homeodomain deleted

Available Since

April 11, 2014

Availability

Academic Institutions and Nonprofits only
pBluescript-mouse eIF2C3

Plasmid

#50355

Purpose

Mouse eIF2C3 cDNA clone

Depositor

Kumiko Ui-Tei

Article

Doi et al Curr Biol. 2003 Jan 8;13(1):41-6.

Insert

eIF2C3 (Ago3 Mouse)

Expression

Bacterial

Promoter

T3

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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sgTrack-Gateway

Plasmid

#114011

Purpose

3rd generation lentiviral empty sgRNA expression vector with a Gateway cassette for co-expression of a reporter cDNA

Depositor

Charles Rudin

Article

Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGK401red-hENT1

Plasmid

#166028

Purpose

Expresses human equilibrative nucleoside transporter 1 (hENT1)

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

human equilibrative nucleoside transporter 1 (SLC29A1 Human)

Use

Synthetic Biology

Expression

Yeast

Promoter

pPGK1

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot10_K

Plasmid

#146775

Purpose

Mammalian Expression of HsNot10

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsNot10 (CNOT10 Human)

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBacPAK p110a RBD DM

Plasmid

#55723

Purpose

His3 GST tagged RBD double mutant p110 alpha T207D, K227A

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

p110 alpha (Pik3ca Mouse)

Tags

His3 GST

Mutation

T207D, K227A

Promoter

polyhedrin promoter

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pGL2-50bp

Plasmid

#16295

Depositor

Wafik El-Deiry

Article

Wang et al Cancer Res. 2006 Jul 15. 66(14):6982-9.

Insert

p53 promoter fragment (TP53 Human)

Use

Luciferase

Expression

Mammalian

Mutation

fragment -38 to +12 relative to first transcripti…

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRII-Topo Plexin D1 in situ probe

Plasmid

#45621

Depositor

Jeffrey Macklis

Article

Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

Insert

Plexin D1 in situ probe (Plxnd1 Mouse)

Use

In situ

Mutation

fragment contains bp# 5751-6406 of NM_026376.3

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
eGFP-RAPH1-F1

Plasmid

#194860

Purpose

Express the RAPH1 fragment (1-535) tagged to eGFP in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

Insert

RAPH1 (RAPH1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

amino acids 1-535

Promoter

CMV

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pUE1-TSP-ORF68 R443A

Plasmid

#162652

Purpose

Expresses ORF68 R443A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Mutation

Amino acid R443 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-TO-HA-SH3BP1 (RNAi-res)

Plasmid

#136645

Purpose

Expression of SH3BP1 in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUC

Depositor

Maria Balda, Karl Matter

Article

Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

Insert

SH3BP1 (RNAi resistant) (SH3BP1 Human)

Tags

HA (MQDLPGNDNSTAG)

Expression

Mammalian

Mutation

GATGACAGCCACCCACTTC changed to AATGACAGCAACACACTTC

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG209

Plasmid

#45109

Depositor

Iain Cheeseman

Article

Gascoigne et al Cell. 2011 Apr 29;145(3):410-22. doi: 10

Insert

CENP-T (aa1-374) Laci-NLS (CENPT Human)

Tags

GFP, Laci-NLS, s peptide, and tev

Expression

Mammalian

Available Since

May 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pTYF-1xSYN-EGFP

Plasmid

#19973

Depositor

Sergey Kasparov

Article

Liu et al BMC Biotechnol. 2008 May 16;8:49. doi: 1

Insert

SYN-EGFP-WPRE (SYN1 jellyfish, Human)

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsXRN1-del1174-1649_AH

Plasmid

#148904

Purpose

Mammalian Expression of HsXRN1-del1174-1649

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2019 Sep 26;47(17):92

Insert

HsXRN1-del1174-1649 (XRN1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4L-Erk1

Plasmid

#39225

Depositor

Melanie Cobb

Article

Frost et al Proc Natl Acad Sci U S A. 1994 Apr 26;91

Insert

Erk1 (MAPK3 Human)

Use

Episomal

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
pBS-KS-attB2-SA(0)-T2A-QF2-Hsp70

Plasmid

#62944

Purpose

Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 0

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-QF2-Hsp70

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pHA-ΔN335-Kin1A

Plasmid

#31606

Depositor

Anthony Brown

Article

Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

Insert

ΔN335 kinesin-1A (Kif5a Mouse)

Tags

HA-tag

Expression

Mammalian

Mutation

deleted amino acids 1-335

Promoter

CMV

Available Since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-p97-EQ-mycStrep

Plasmid

#31838

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Tags

Strep and myc

Expression

Bacterial and Mammalian

Mutation

E578Q

Promoter

CMV

Available Since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only
plexm-mouse LRRC33

Plasmid

#111601

Purpose

Express mouse LRRC33 in 293T cells

Depositor

Timothy Springer

Article

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

mouse LRRC33 (Nrros Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Starts at aa 18

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSicoR human Drosha3

Plasmid

#14768

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Drosha shRNA (DROSHA Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
pDBEcR/Bla

Plasmid

#201556

Purpose

Plasmid with SB ITRs for expressing DBEcR (3xmyc-tagged) under EF1α promoter; blasticidin resistance.

Depositor

Randy Poon

Article

Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

Insert

DBEcR

Use

Unspecified; Sleeping beauty transposon

Tags

Myc

Expression

Mammalian

Promoter

EF1a

Available Since

July 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-KS-attB2-SA(2)-T2A-QF2-Hsp70

Plasmid

#62946

Purpose

Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-QF2-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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