We narrowed to 6,994 results for: Cad

1. pSAVED-CHAT_PCaspase_PCi_PC-σ

   Plasmid

   #214040

   Purpose

   Bacterial expression plasmid for SAVED-CHAT, PCaspase, PCi, and PC-σ from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT-Pcaspase-Pci-PC-σ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   lacUV5 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOPINO_H11

   Plasmid

   #184277

   Purpose

   Expresses nanobody H11 in E. coli

   Depositor

   Ray Owens

   Article

   Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

   Insert

   Nanobody H11
   

   Use

   Tags

   His6

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   T7

   Available since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. WT-β3

   Plasmid

   #166579

   Purpose

   For expression of cytoplasmic tail of mouse beta3 integrin with N-terminal GST fusion protein in bacterial cells.

   Depositor

   Vesa Hytönen

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2020 Dec 22;11

   Insert

   β3 (Itgb3 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-hRedOpsin-fCD200

   Plasmid

   #164425

   Purpose

   AAV plasmid expressing full-length CD200 in photoreceptors

   Depositor

   Connie Cepko

   Article

   Wang et al Proc Natl Acad Sci U S A. 2019 May 14;11

   Insert

   CD200 (Cd200 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human red opsin

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. OR2M4_Deletion_gRNA

   Plasmid

   #195194

   Purpose

   dual gRNAs for deletion of OR2M4 in a third generation Cas9 backbone with GFP

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   OR2M4 dual gRNA (OR2M4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET32a-HD39Q

   Plasmid

   #11514

   Depositor

   Pamela Bjorkman

   Article

   Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

   Insert

   huntingtin, exon 1, 39 glutamines (HTT Human)
   

   Use

   Tags

   His, His (out of frame), and Trx

   Expression

   Bacterial

   Mutation

   huntingtin exon 1 with 39 glutamines, vector m…

   Promoter

   Available since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pSCT-GAL93-NONO

   Plasmid

   #46325

   Depositor

   Steven Brown

   Article

   Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

   Insert

   NONO (NONO Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 11, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pENTR1A mKate2-hu_ncOGT 5N5A

   Plasmid

   #154285

   Purpose

   Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; 5N5A variant, no HCF-1 cleavage)

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Gateway entry vector

   Tags

   2x FLAG and mKate2

   Expression

   Mutation

   N322A, N356A, N390A, N424A, N458A mutations (HCF-…

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTET-IUPi

   Plasmid

   #122019

   Purpose

   Expresses IUP pathway genes (ChK, IPK, idi) under inducible promoter. aTC inducible. Kan. resist.

   Depositor

   Gregory Stephanopoulos

   Article

   Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

   Inserts

   Choline kinase (CKI1 Budding Yeast)
   Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
   isopentenyl-diphosphate Delta-isomerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E. coli

   Promoter

   pTET

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCytERM-mGreenLantern

    Plasmid

    #164467

    Purpose

    Visualization of the ER in cells (compatible with OSER assay)

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Use

    Tags

    Cytochrome p450 first 29 animo acids (Oryctolagus…

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CMV

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Beclin-BATS

    Plasmid

    #30498

    Depositor

    Qing Zhong

    Article

    Fan et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Insert

    Beclin, Barkor (BECN1, ATG14 Human)
    

    Use

    Tags

    3xFlag and hrGFP

    Expression

    Mammalian

    Mutation

    Beclin 1 + 413-492 aa of Barkor

    Promoter

    Available since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pBabe RFP-DN-FOXO1-HA neo

    Plasmid

    #45813

    Depositor

    Kevin Janes

    Article

    Wang et al Proc Natl Acad Sci U S A. 2011 Oct 4;108

    Insert

    FOXO1 (FOXO1 Human)
    

    Use

    Retroviral

    Tags

    HA and RFP

    Expression

    Mammalian

    Mutation

    aa1-255

    Promoter

    Available since

    June 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK-ATF4 1-275

    Plasmid

    #26118

    Depositor

    Yihong Ye

    Article

    Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

    Insert

    ATF4 (ATF4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    aa's 276-351 have been deleted

    Promoter

    Available since

    March 1, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pSico Dnmt1

    Plasmid

    #12165

    Depositor

    Tyler Jacks

    Article

    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

    Insert

    Dnmt1 shRNA (Dnmt1 Mouse)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    EGFP is expressed from this plasmid as a marker, …

    Promoter

    Available since

    June 30, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pBabe puro-HA-PIK3CA(H1047R;F486S;del)

    Plasmid

    #33255

    Depositor

    Thomas Roberts

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Retroviral

    Tags

    Hemagglutinin (HA)

    Expression

    Mammalian

    Mutation

    changed histidine 1047 to arginine and phenylalan…

    Promoter

    SV40

    Available since

    Jan. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pBItetDT240GFP

    Plasmid

    #96904

    Purpose

    tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 240 interrupted CTG repeats in exon 15 located

    Depositor

    Thomas Cooper

    Article

    Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

    Insert

    EGFP and human DMPK exons 11-15 with 240 interrupted CTG repeats (DMPK Human)
    

    Use

    Tetracycline responsive and bidirectional

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetracycline responsive and bidirectional

    Available since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-mScarlet-SARS-CoV-2-SL1 alone

    Plasmid

    #191485

    Purpose

    Lentiviral expression of SARS-CoV-2 5_ UTR SL1 alone

    Depositor

    Hao Wu

    Article

    Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

    Insert

    SARS-CoV-2 5' UTR SL1 alone (NEWENTRY Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. mitoTALECD for_atp1_1397CN

    Plasmid

    #186203

    Purpose

    For genome editing (base editing) of Arabidopsis mitochondria, targeted C of RNA editing site of ATP1 by OTP87

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11

    Inserts

    mitochondrial targeted TALE left hand with 1397C of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1397N of Cytidine Deaminase (mitoTALECD)
    

    Use

    Synthetic Biology; Ti plasmid for plant transform…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. tdEos-Vimentin-7

    Plasmid

    #57688

    Purpose

    Localization: Intermediate Filaments, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    Vimentin (VIM Human)
    

    Use

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pcDNA3.1+ Flag-RAGB99L

    Plasmid

    #128101

    Purpose

    Expresses constitutively GTP-bound RAGB in mammalian cells in a G418-selectable plasmid

    Depositor

    Chi Van Dang

    Article

    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

    Insert

    Ras related GTP binding B (RRAGB Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Q99L (CAA to CTA)

    Promoter

    CMV

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. G-GECO1.2-Orai1Y80E

    Plasmid

    #73565

    Purpose

    Green fluorescent reporter of mutant Orai1Y80E-associated calcium influx

    Depositor

    Michael Cahalan

    Article

    Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

    Insert

    G-GECO1.2-Orai1Y80E (ORAI1 Synthetic, Human)
    

    Use

    Tags

    G-GECO1.2

    Expression

    Mammalian

    Mutation

    changed tyrosine 80 of Orai1 to glutamate

    Promoter

    CMV Immediate Early

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. pRK-ATF4 111-351

    Plasmid

    #26119

    Depositor

    Yihong Ye

    Article

    Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

    Insert

    ATF4 (ATF4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    aa's 1-110 have been deleted

    Promoter

    Available since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

23. 1D3 HC

    Plasmid

    #170670

    Purpose

    Mammalian Expression Plasmid of Anti-cytochrome c, 1D3 HC (Human)

    Depositor

    Rafel Radi, Ronald Jemmerson

    Article

    Tomasina et al Proc Natl Acad Sci U S A. 2022 Nov 22;11

    Insert

    HC of anti-cytochrome c, 1D3 (human) recombinant mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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24. 1D3 LC

    Plasmid

    #170669

    Purpose

    Mammalian Expression Plasmid of Anti-cytochrome c, 1D3 LC (Human)

    Depositor

    Rafel Radi, Ronald Jemmerson

    Article

    Tomasina et al Proc Natl Acad Sci U S A. 2022 Nov 22;11

    Insert

    LC of anti-cytochrome c, 1D3 (human) recombinant mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pMXs-ms-c-Myc-V394D

    Plasmid

    #50775

    Purpose

    retroviral expression of mouse c-Myc V394D mutant

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

    Insert

    c-Myc V394D (Myc Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    V394D (does not bind to Miz-1)

    Promoter

    Available since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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26. pRK5-H1-shRNA-CMV-HA-PACSIN1

    Plasmid

    #72577

    Purpose

    Bicistronic construct expressing PACSIN1 shRNA and HA-PACSIN1-rescue construct

    Depositor

    Richard Huganir

    Article

    Anggono et al Proc Natl Acad Sci U S A. 2013 Aug 20;11

    Inserts

    PACSIN1 (Pacsin1 Mouse)
    H1-PACSIN1 shRNA cassette
    

    Use

    RNAi

    Tags

    HA

    Expression

    Mammalian

    Mutation

    5 silent mutations around shRNA recognition seque…

    Promoter

    CMV and H1

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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27. pRK-ATF4 248-351

    Plasmid

    #26120

    Depositor

    Yihong Ye

    Article

    Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

    Insert

    ATF4 (ATF4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    ATF4 aa's 1-247 have been deleted

    Promoter

    Available since

    Jan. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

28. mILK-R255A/R349A-EFGP

    Plasmid

    #176899

    Purpose

    mouse ILK with R255A and R349A point mutations cloned into pEGFP-N1

    Depositor

    Sara Wickström

    Article

    Martin et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

    Insert

    Integrin-linked kinase (Ilk Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Arginine 255 to Alanine and Arginine 349 to Alani…

    Promoter

    CNV

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. pRetroX-Tight-Pur-Trim69 Full length, isoform A (codon optimized)

    Plasmid

    #199566

    Purpose

    Stable and dox. inducible expression of Trim69 Full length, isoform A (codon optimized) after retroviral-mediated gene transfer

    Depositor

    Andrea Cimarelli, Yuxin Song

    Article

    Song et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

    Insert

    Trim69 Full length, isoform A (codon optimized) (TRIM69 Human)
    

    Use

    Retroviral; Allows for puromycin selection

    Tags

    Flag

    Expression

    Mutation

    codon optimized

    Promoter

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PB-hSyn-mSyp-RT1-24xGCN4

    Plasmid

    #106300

    Purpose

    The plasmid encodes mouse Synaptophysin sparsely labeled with SunTag24x_v4.

    Depositor

    James Zhe Liu

    Article

    Liu et al Proc Natl Acad Sci U S A. 2017 Dec 28. p

    Insert

    mSyp-RT1-24xGCN4 (Syp Mouse)
    

    Use

    Tags

    24xGCN4

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin1

    Available since

    March 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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