We narrowed to 23,146 results for: REP

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pBMNb-cALFA

Plasmid

#159991

Purpose

For the protein expression with ALFA tag at C-terminus in mammalian cells

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Type

Empty backbone

Tags

HRV3C protease cleavage site-ALFA tag-twin Strep …

Expression

Mammalian

Mutation

CMV promoter, WPRE

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pUC18-mini-Tn7T-Plac-dCas9 (Plac)

Plasmid

#105235

Purpose

Tn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Plac promoter for expression in P. aeruginosa

Depositor

Kristala Prather

Article

Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

Insert

S. pasteurianus dCas9

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Bacterial

Mutation

D10A, H599A

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCDF-1b-RFC140ΔN555

Plasmid

#175043

Purpose

Overexpression of human (Rfc1) p140ΔN555 in E.coli

Depositor

Brian Kelch

Article

Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

Replication factor C subunit 1 (RFC1 Human)

Expression

Bacterial

Promoter

T7 promoter

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP‐RNASEH2C

Plasmid

#108698

Purpose

For expression of N-terminally eGFP-tagged human RNASEH2C in mammalian cells

Depositor

Andrew Jackson, Martin Reijns

Article

Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

Insert

RNASEH2C (RNASEH2C Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pNvT-MHC::mCh

Plasmid

#67943

Purpose

Transgenesis vector consisting of a 1.6-kb fragment of MyHC1 promoter region upstream of the start codon and an mCherry reporter gene, flanked by inverted binding sites of meganuclease I-SceI

Depositor

Ulrich Technau

Article

Renfer et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

Insert

mCherry

Use

Transgenic reporter in the sea anemone, nematoste…

Promoter

Myosin Heavy Chain1 (MyHC1)

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pHD1

Plasmid

#30974

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

repeat HD1

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pDRM210 LGP (Luciferase GFP Puro)

Plasmid

#174722

Purpose

Expresses the firefly luciferase gene, E2A, eGFP, F2A, and the puromycin resistance gene

Depositor

Wayne Tilley

Article

Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

Inserts

Firefly Luciferase
eGFP
pac

Use

Lentiviral and Luciferase

Tags

E2A linker and F2A linker

Expression

Mammalian

Promoter

MND, MND (off Luc-E2A), and MND (off Luc-E2A-eGFP…

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCR2.1 Clover-LMNA Donor

Plasmid

#122508

Purpose

Homology repair template for in frame (first exon) clover knock-in of human LMNA gene

Depositor

Graham Dellaire

Article

Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

Insert

Homology Repair Template for Human LMNA (N-Terminal Clover Tag) (LMNA Human)

Use

Homology repair template plasmid (donor plasmid)

Tags

Clover

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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eBFP2 (eBFP2)_CD

Plasmid

#66034

Purpose

MoClo Basic Part: CDS - Fluorescent protein. Blue. Derived from Addgene#14891. [C:eBFP2:D]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Fluorescent reporter - BFP

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pTH-PanCen-mVenus

Plasmid

#49640

Purpose

TALEColor-mVenus-Pan-Centromere

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2013 Dec 9.

Type

Empty backbone

Use

TALEN

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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ptubP-QS

Plasmid

#24352

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

QS

Tags

white gene

Expression

Insect

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf106

Plasmid

#12794

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf106

Expression

Mammalian

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pME18ST-d5-HT2B

Plasmid

#53110

Purpose

Expression of Drosophila 5-HT2B

Depositor

Leslie Vosshall

Article

Gasque et al Sci Rep. 2013;3:srep02120. doi: 10.1038/

Insert

5HT2B (CG42796 Fly)

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
pME18ST-d5-HT7

Plasmid

#53111

Purpose

Expression of Drosophila 5-HT7

Depositor

Leslie Vosshall

Article

Gasque et al Sci Rep. 2013;3:srep02120. doi: 10.1038/

Insert

5HT7 (5-HT7 Fly)

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
XW12

Plasmid

#65834

Purpose

Pquas-Δpes10-GFP-3'UTR-unc-54

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

5XQUAS-delta-pes-10

Tags

GFP

Expression

Worm

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pOTTC589 - pAAV c-fos Nuc-eYFP

Plasmid

#59133

Purpose

An AAV vector that expresses nuclear localized eYFP under control of the c-fos promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Has Service

AAV1

Insert

Nuclear-localized Enhanced Yellow Fluorescent Protein

Use

AAV

Tags

3xNLS

Expression

Mammalian

Promoter

mFos

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS Donor mClover-LMNA

Plasmid

#98970

Purpose

Donor for endogenous N-ter tagging of hLMNA with mClover. Cas9 Homologous Recombination Reporter

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA5'UTR-mClover-LMNA5'exon1 (LMNA Human)

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pLV.MRE11i

Plasmid

#15565

Depositor

Didier Trono

Article

Ariumi et al J Virol. 2006 Mar . 80(5):2445-52.

Insert

MRE11 shRNA (MRE11 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 10, 2007

Availability

Academic Institutions and Nonprofits only
pHLmMBP-6

Plasmid

#72346

Purpose

Mammalian expression of cytoplasmic mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

Depositor

Luca Jovine

Article

Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

Type

Empty backbone

Tags

6His-tag, HA-tag, Strep-tag II, and mMBP

Expression

Mammalian

Promoter

CMV enhancer + chicken beta-actin promoter

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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EF1a_GATA6_P2A_Hygro_Barcode

Plasmid

#120445

Purpose

Barcoded lentiviral vector to express GATA6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

GATA6 (GATA6 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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