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BBa_J72110-BBa_J72094

Plasmid

#40778

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

AraC-pBad + coi

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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BBa_J72110-BBa_J72097

Plasmid

#40779

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

RhaRS-pRhaB + Coi

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pCS2vegf121

Plasmid

#22416

Depositor

Nathan Lawson

Article

Lawson et al Dev Cell. 2002 Jul . 3(1):127-36.

Insert

vegf (vegfaa Zebrafish)

Use

Zebrafish expression

Available Since

Jan. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pJL051

Plasmid

#242609

Purpose

Parental vector for building the dual-sgRNA CRISPRi library. modified mU6-sgRNA library vector

Depositor

Brittany Adamson

Article

Simpson et al bioRxiv [Preprint]. 2023 Aug 20:2023.08.

Insert

sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

modified mouse U6

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pJL052

Plasmid

#242610

Purpose

Parental vector for building the dual-sgRNA CRISPRi library. modified hU6-sgRNA library vector

Depositor

Brittany Adamson

Article

Simpson et al bioRxiv [Preprint]. 2023 Aug 20:2023.08.

Insert

sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

modified human U6

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pBAD-CC-Di-C-Venus

Plasmid

#190710

Purpose

Encodes the parallel coiled-coil CC-Di followed by C-Venus.

Depositor

Nigel Savery

Article

Rhys et al Nat Chem Biol. 2022 Jul 14. pii: 10.1038

Insert

CC-Di-C-Venus

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-C-Venus-CC-Di

Plasmid

#190712

Purpose

Encodes C-Venus followed by the parallel coiled-coil CC-Di.

Depositor

Nigel Savery

Article

Rhys et al Nat Chem Biol. 2022 Jul 14. pii: 10.1038

Insert

C-Venus-CC-Di

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Lifeact-7

Plasmid

#54721

Purpose

Localization: Actin, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Lifeact-mCerulean3

Expression

Mammalian

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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ptdTomato-SMCR8

Plasmid

#83438

Purpose

Expresses SMCR8-tdTomato in mammalian cells

Depositor

Shawn Ferguson

Article

Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

Insert

Human SMCR8 (SMCR8 Human)

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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BBa_J72114-BBa_J72104

Plasmid

#40783

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

RhaRS + pRhaB + LuxCDABE, VioABCE, LacZ

Use

Luciferase and Synthetic Biology; Phagemid

Expression

Bacterial

Available Since

Jan. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pET28a-IMX-DogTag

Plasmid

#113765

Purpose

Expresses IMX-DogTag in bacterial cells for modular antigen multimerization using SnoopLigase

Depositor

Mark Howarth

Article

Andersson et al Sci Rep. 2019 Mar 15;9(1):4625. doi: 10.

Insert

IMX-DogTag

Expression

Bacterial

Promoter

T7

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Rab5a-7

Plasmid

#55444

Purpose

Localization: Ras GTPase, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Rab5a

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
BBa_J72110-BBa_J72103

Plasmid

#40780

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

AraC-pBad + coi + cin + repL + pacA

Use

Synthetic Biology; Phagemid

Expression

Bacterial

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pPB-mCherry-SopF

Plasmid

#229745

Purpose

Expresses mCherry-tagged SopF in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

mCherry-SopF

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD-C-Venus-apCC-Di

Plasmid

#190711

Purpose

Encodes C-Venus followed by the antiparallel coiled-coil apCC-Di.

Depositor

Nigel Savery

Article

Rhys et al Nat Chem Biol. 2022 Jul 14. pii: 10.1038

Insert

C-Venus-apCC-Di

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-C-Venus

Plasmid

#190713

Purpose

Control plasmid encoding C-Venus, but without a coiled-coil to drive reconstitution.

Depositor

Nigel Savery

Article

Rhys et al Nat Chem Biol. 2022 Jul 14. pii: 10.1038

Insert

C-Venus

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-apCC-Di-C-Venus

Plasmid

#190709

Purpose

Encodes the antiparallel coiled-coil apCC-Di followed by C-Venus.

Depositor

Nigel Savery

Article

Rhys et al Nat Chem Biol. 2022 Jul 14. pii: 10.1038

Insert

apCC-Di-C-Venus

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mCerulean3-Lifeact-7

Plasmid

#54721

Purpose

Localization: Actin, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Lifeact-mCerulean3

Expression

Mammalian

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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ptdTomato-SMCR8

Plasmid

#83438

Purpose

Expresses SMCR8-tdTomato in mammalian cells

Depositor

Shawn Ferguson

Article

Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

Insert

Human SMCR8 (SMCR8 Human)

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
BBa_J72114-BBa_J72104

Plasmid

#40783

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

RhaRS + pRhaB + LuxCDABE, VioABCE, LacZ

Use

Luciferase and Synthetic Biology; Phagemid

Expression

Bacterial

Available Since

Jan. 4, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge