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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,521 results for: RAP

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Showing: 3781 - 3800 of 8521 results

  8. pPP381

    Plasmid
    #158798
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    dCasPhi-2
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    RuvC-inactivating mutation D394A
    Available Since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158798-plasmid-map-sequence-id-309523

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  14. pCali2

    Plasmid
    #86119
    Purpose
    Multiplex temperature-gated expression of mWasabi and mCherry reporters
    Depositor
    Mikhail Shapiro
    Article
    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi
    Inserts
    TlpA36
    mWasabi
    TcI
    mCherry
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    A67T and D341A, P60L, D135V, K187R, K202I, L208Q
    Promoter
    pR/pL, pR/pL, pLacI, pTlpA, and pTlpA, pLacI
    Available Since
    Feb. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86119-plasmid-map-sequence-id-173197

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  15. Mut1-RS

    Plasmid
    #182881
    Purpose
    A pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut1-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.
    Depositor
    Miriam Amiram
    Article
    Hadar et al Chembiochem. 2021 Apr 16;22(8):1379-1384
    Insert
    Mut1-RS
    Expression
    Bacterial
    Available Since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    182881-plasmid-map-sequence-id-366057

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  16. pPP537

    Plasmid
    #158803
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally 2xSV40 NLS and hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    CasPhi-2
    Tags
    2xSV40NLS-6xHis
    Expression
    Bacterial
    Available Since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158803-plasmid-map-sequence-id-309579

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Showing: 3781 - 3800 of 8521 results