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lenti dCAS-VP64_Blast (Lentiviral Prep)
Viral Prep#61425-LVPurposeReady-to-use Lentiviral Prep particles produced from lenti dCAS-VP64_Blast (#61425). In addition to the viral particles, you will also receive purified lenti dCAS-VP64_Blast plasmid DNA.DepositorPromoterEF1aTagsNoneAvailable sinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Sumo TEV LIC cloning vector (2S-T)
Plasmid#29711DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTags6xHis, SUMO, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Sumo TEV LIC cloning vector (2S-T)
Plasmid#29711DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTags6xHis, SUMO, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_ATG16L1_WT
Plasmid#82255PurposeGateway Donor vector containing ATG16L1 , part of the Target Accelerator Plasmid Collection.DepositorInsertATG16L1 (ATG16L1 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_ATG16L1_WT
Plasmid#82255PurposeGateway Donor vector containing ATG16L1 , part of the Target Accelerator Plasmid Collection.DepositorInsertATG16L1 (ATG16L1 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
MIP 339 CoMiP coLin28 2A Nanog IRES eGFP
Plasmid#63729PurposeTo express codon optimized Lin-28 and wild type Nanog. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).UseTagsT2AExpressionMammalianMutationcodon optimizedPromoterSFFVAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
MIP 339 CoMiP coLin28 2A Nanog IRES eGFP
Plasmid#63729PurposeTo express codon optimized Lin-28 and wild type Nanog. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).UseTagsT2AExpressionMammalianMutationcodon optimizedPromoterSFFVAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
Zebrabow-GateDest
Plasmid#118221PurposeFor cloning promoter into a Zebrabow vector with Gateway reaction. Only compatible with single site Gateway. Not compatible with Tol2-kit.DepositorInsertBrainbow 1.0 L - ccdB survival cassette
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Zebrabow-GateDest
Plasmid#118221PurposeFor cloning promoter into a Zebrabow vector with Gateway reaction. Only compatible with single site Gateway. Not compatible with Tol2-kit.DepositorInsertBrainbow 1.0 L - ccdB survival cassette
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
AcGFP1-C1
Plasmid#54607PurposeLocalization: C1 Cloning Vector, Excitation: 475, Emission: 505DepositorTypeEmpty backboneUseTagsAcGFP1ExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
AcGFP1-C1
Plasmid#54607PurposeLocalization: C1 Cloning Vector, Excitation: 475, Emission: 505DepositorTypeEmpty backboneUseTagsAcGFP1ExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn_CapChR2_eYFP
Plasmid#188035PurposeExpresses CapChR2-eYFP in mammalian neuronsDepositorInsertCapChR2-eYFP
UseAAVTagseYFPExpressionMutationS43D, L112C, T139C and N238E mutations in CoChRPromoterhSynAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP18
Plasmid#22572DepositorInsertUSP18 (USP18 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP18
Plasmid#22572DepositorInsertUSP18 (USP18 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
lenti-EF1a-dCas9-KRAB-Puro
Plasmid#99372Purpose3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)DepositorInsertdCas9-KRAB-T2A-Puro
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
lenti-EF1a-dCas9-KRAB-Puro
Plasmid#99372Purpose3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)DepositorInsertdCas9-KRAB-T2A-Puro
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Rac1-2G
Plasmid#66111PurposeSecond generation Rac1 FRET biosensor for lentivirus productionDepositorInsertSecond generation Rac1 FRET biosensor
UseLentiviralTagsHis-MycExpressionMammalianMutationPromoterCMVAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Rac1-2G
Plasmid#66111PurposeSecond generation Rac1 FRET biosensor for lentivirus productionDepositorInsertSecond generation Rac1 FRET biosensor
UseLentiviralTagsHis-MycExpressionMammalianMutationPromoterCMVAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-dCas9-BFP-KRAB
Plasmid#46911PurposeHuman expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domainDepositorInsertdCas9-BFP-KRAB fusion
UseCRISPR and LentiviralTags2xNLS, BFP, HA, and KRAB domainExpressionMammalianMutationPromoterSFFVAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-dCas9-BFP-KRAB
Plasmid#46911PurposeHuman expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domainDepositorInsertdCas9-BFP-KRAB fusion
UseCRISPR and LentiviralTags2xNLS, BFP, HA, and KRAB domainExpressionMammalianMutationPromoterSFFVAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTREtight2
Plasmid#19407DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTREtight2
Plasmid#19407DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Myc-Foxo1 AAA (pCMV5)
Plasmid#17547DepositorInsertFoxo1 AAA (Foxo1 Mouse)
UseTagsMycExpressionMammalianMutationChanged T24A T253A S316APromoterCMVAvailable sinceOct. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
Myc-Foxo1 AAA (pCMV5)
Plasmid#17547DepositorInsertFoxo1 AAA (Foxo1 Mouse)
UseTagsMycExpressionMammalianMutationChanged T24A T253A S316APromoterCMVAvailable sinceOct. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
FLAG-LoxP-PURO-LoxP-SNAP-TERT
Plasmid#71390PurposeHomologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes PURO resistance flanked by LoxP sites for selection.DepositorInsertTERT (TERT Human)
UseHr donorTagsFLAG-SNAPExpressionMutationPromoterEndogenous TERT promotorAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
FLAG-LoxP-PURO-LoxP-SNAP-TERT
Plasmid#71390PurposeHomologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes PURO resistance flanked by LoxP sites for selection.DepositorInsertTERT (TERT Human)
UseHr donorTagsFLAG-SNAPExpressionMutationPromoterEndogenous TERT promotorAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
FUGW-Cytosol-TurboID
Plasmid#200961Purpose3rd gen lentiviral plasmid for expressing cytosol localized TurboID with EGFPDepositorInsertCytosol-TurboID
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-N1
Plasmid#54642PurposeLocalization: N1 Cloning Vector, Excitation: 554, Emission: 581DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagstdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-N1
Plasmid#54642PurposeLocalization: N1 Cloning Vector, Excitation: 554, Emission: 581DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagstdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEntr_Myc-BirA-G3-ER
Plasmid#173814PurposePromiscuous biotin ligase (BirA-G3) targeted to ER lumen in Gateway entry vector. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage siteDepositorInsertMyc-BirA-G3-ER
UseGateway shuttle vectorTagsMycExpressionMutationPromoterAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-29: LMNB1-mTagRFP-T
Plasmid#114403PurposeHomology arms and mTagRFPT-linker sequence for N-terminus tagging of human LMNB1DepositorInsertLMNB1 Homology Arms with mTagRFPT-linker (LMNB1 Human)
UseCRISPR; Donor templateTagsmTagRFPT-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceSept. 4, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
AICSDP-29: LMNB1-mTagRFP-T
Plasmid#114403PurposeHomology arms and mTagRFPT-linker sequence for N-terminus tagging of human LMNB1DepositorInsertLMNB1 Homology Arms with mTagRFPT-linker (LMNB1 Human)
UseCRISPR; Donor templateTagsmTagRFPT-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceSept. 4, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pMK1334
Plasmid#127965PurposesgRNA expression vector compatible with CROP-Seq and imaging screensDepositorInsertEF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceAug. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK1334
Plasmid#127965PurposesgRNA expression vector compatible with CROP-Seq and imaging screensDepositorInsertEF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceAug. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX304 CMV FKBP-V5-sTurboID-high affinity (N)
Plasmid#153004PurposeExpresses high-affinity split-TurboID N-terminal fragment in mammalian cellsDepositorInsertsTurboID-high affinity (N) (G78)
UseLentiviralTagsFKBP and V5ExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX304 CMV FKBP-V5-sTurboID-high affinity (N)
Plasmid#153004PurposeExpresses high-affinity split-TurboID N-terminal fragment in mammalian cellsDepositorInsertsTurboID-high affinity (N) (G78)
UseLentiviralTagsFKBP and V5ExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
Kif5A-GFP-CIBN
Plasmid#102252PurposeExpresses fusion of kinesin heavy chain 5A (1-572) with GFP and CIB1 (1-170)DepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only
